ID  F1MSG6;
PN  Rap guanine nucleotide exchange factor 2;
GN  RAPGEF2;
OS  9913;
SL  Nucleus Position: SL-0198;
SL  Comments: Cytoplasm {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000250}. Cell membrane {ECO:0000250}. Late endosome {ECO:0000250}. Cell junction {ECO:0000250}. Note=Associated with the synaptic plasma membrane. Localized diffusely in the cytoplasm before neuronal growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with the high affinity nerve growth factor receptor NTRK1 at late endosomes. Translocated to the perinuclear region in a RAP1A-dependent manner. Translocated to the cell membrane. Colocalized with CTNNB1 at cell-cell contacts (By similarity). {ECO:0000250}.
DR  UNIPROT: F1MSG6;
DR  Pfam: PF00595;
DR  Pfam: PF00788;
DR  Pfam: PF00617;
DR  Pfam: PF00618;
DR  PROSITE: PS50042;
DR  PROSITE: PS50106;
DR  PROSITE: PS50200;
DR  PROSITE: PS50009;
DR  PROSITE: PS50212;
DE  Function: Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B- Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions (By similarity). Binds to cAMP. {ECO:0000250, ECO:0000269|PubMed:23800469}.
DE  Reference Proteome: Yes;
DE  Interaction: Q02248; IntAct: EBI-6927091; Score: 0.50
DE  Interaction: Q0VCX4; IntAct: EBI-6927177; Score: 0.40
GO  GO:0016324;
GO  GO:0005911;
GO  GO:0005737;
GO  GO:0030139;
GO  GO:0005770;
GO  GO:0043005;
GO  GO:0043025;
GO  GO:0048471;
GO  GO:0005886;
GO  GO:0032991;
GO  GO:0045202;
GO  GO:0031697;
GO  GO:0030552;
GO  GO:0005096;
GO  GO:0005085;
GO  GO:0030165;
GO  GO:0050699;
GO  GO:0071880;
GO  GO:0001568;
GO  GO:0031547;
GO  GO:0019933;
GO  GO:0071320;
GO  GO:0071321;
GO  GO:1990090;
GO  GO:0061028;
GO  GO:0021884;
GO  GO:0007186;
GO  GO:0008285;
GO  GO:0050774;
GO  GO:0048022;
GO  GO:0038180;
GO  GO:0001764;
GO  GO:0031175;
GO  GO:0007218;
GO  GO:2000481;
GO  GO:0043950;
GO  GO:2000670;
GO  GO:0070374;
GO  GO:0043547;
GO  GO:2001224;
GO  GO:0010976;
GO  GO:0032092;
GO  GO:0045860;
GO  GO:2001214;
GO  GO:0032486;
GO  GO:0007265;
GO  GO:1901888;
GO  GO:0021591;
TP  Membrane Topology: Unknown; Source: UniProt - Sequence Analysis;
SQ  MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPE
SQ  TAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAG
SQ  TIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEK
SQ  NMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLCSPMEVGKKLLEWF
SQ  NDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPAREAPLPFI
SQ  LLGGSEKGFGIFVDSVDSASKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEE
SQ  KRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQD
SQ  DSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVVQAIRE
SQ  FAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVE
SQ  VATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKTFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRE
SQ  CKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFL
SQ  HEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSS
SQ  FLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPTDKKPVKSETSPVAPRAGLQPKAQPQPQP
SQ  PQPPHKLNQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRRPAEDTISNTSSQLSSPPTSPQS
SQ  SPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSLPVSLPDERRQRPSVSIVETSLASGRLERRPAVEPDQYSLG
SQ  SCAPLSESRGLYAAATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYPG
SQ  DAAGLWASSSHMDQIMFPDHSAKYSRQSQSRESLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSLDADSSSMTA
SQ  VTAEEAKPAAMAAHIAVTPSAAKGLIARKEGRYREPPPTPPGYVGIPITDFPEAHPHPARKPPDYTVALQRSRMLARPAE
SQ  PPAPGSARPAPRPQWHRPGDGDPRAGPCAPPGLTAEEDEDEQVSAV
//