ID  O61955;
PN  Eukaryotic translation initiation factor 4E-3;
GN  ife;
OS  6239;
SL  Nucleus Position: SL-0198;
SL  Comments: Cytoplasmic granule {ECO:0000269|PubMed:31147388}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:31147388, ECO:0000269|PubMed:31216475}. Note=Localizes to cytoplasmic granules in early embryos (PubMed:31147388). Localizes to puncta in the perinuclear region in the germline syncytium (PubMed:31216475, PubMed:31147388). {ECO:0000269|PubMed:31147388, ECO:0000269|PubMed:31216475}.
DR  UNIPROT: O61955;
DR  UNIPROT: Q8MNX5;
DR  UNIPROT: Q95X31;
DR  PDB: 5ABX;
DR  PDB: 5ABY;
DR  Pfam: PF01652;
DR  PROSITE: PS00813;
DE  Function: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. All 5 eIF4E proteins bind monomethyl cap structures. Only ife-1, ife-2 and ife-5 bind trimethyl cap structures which result from trans-splicing. Translation of trimethyl cap structure mRNAs may be regulated by intracellular redox state; disulfide bonds change the width and depth of the cap-binding cavity determining selectivity to mRNA caps. Ife-3 is essential for viability. Component of the pid-1 and tost-1 variants of the PETISCO complexes, which have roles in the biogenesis of a class of 21 nucleotide PIWI- interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) and embryogenesis, respectively (PubMed:31147388, PubMed:31216475). Within the pid-1 variant of the PETISCO complex binds to capped 21U-RNA precursor molecules, possibly playing a role in the processing of the 5' end of the molecules to promote binding of other complex components such as pid-3 (PubMed:31147388). However, it is not essential for the biogenesis of 21U-RNAs by itself (PubMed:31147388). Within the tost-1 variant of the PETISCO complex binds to splice leader SL1 RNA fragments to possibly play a role in their processing (PubMed:31147388). {ECO:0000269|PubMed:10744754, ECO:0000269|PubMed:12422237, ECO:0000269|PubMed:31147388, ECO:0000269|PubMed:9553113, ECO:0000305|PubMed:31216475}.
DE  Reference Proteome: Yes;
DE  Interaction: Q9XW13; IntAct: EBI-2411755; Score: 0.62
DE  Interaction: Q20898; IntAct: EBI-2411740; Score: 0.62
DE  Interaction: Q22497; IntAct: EBI-2411744; Score: 0.62
DE  Interaction: Q09293; IntAct: EBI-21449250; Score: 0.52
DE  Interaction: Q1ZXS5; IntAct: EBI-6457351; Score: 0.37
DE  Interaction: G5EDU4; IntAct: EBI-6458345; Score: 0.37
DE  Interaction: Q19541; IntAct: EBI-21448974; Score: 0.46
DE  Interaction: O76616; IntAct: EBI-21449031; Score: 0.35
DE  Interaction: Q20057; IntAct: EBI-21449069; Score: 0.35
DE  Interaction: Q9N405; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: G5EC16; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: O01871; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: G5ED26; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: O45551; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: Q18603; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: Q94279; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: Q18244; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: Q21323; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: P48166; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: Q18490; IntAct: EBI-21449322; Score: 0.35
DE  Interaction: Q22624; IntAct: EBI-21449322; Score: 0.35
GO  GO:0016281;
GO  GO:0048471;
GO  GO:0034518;
GO  GO:0070992;
GO  GO:0000340;
GO  GO:0003743;
GO  GO:0034585;
GO  GO:0009792;
GO  GO:0031047;
GO  GO:0006413;
TP  Membrane Topology: Unknown; Source: UniProt - Sequence Analysis;
SQ  MSTSVAENKALSASGDVNASDASVPPELLTRHPLQNRWALWYLKADRNKEWEDCLKMVSLFDTVEDFWSLYNHIQSAGGL
SQ  NWGSDYYLFKEGIKPMWEDVNNVQGGRWLVVVDKQKLQRRTQLLDHYWLELLMAIVGEQFDEYGDYICGAVVNVRQKGDK
SQ  VSLWTRDATRDDVNLRIGQVLKQKLSIPDTEILRYEVHKDSSARTSSTVKPRICLPAKDPAPVKEKGPAATTSPSNPGTE
SQ  ATGTSPATPTP
//