ID  P04406;
PN  Glyceraldehyde-3-phosphate dehydrogenase;
GN  GAPDH;
OS  9606;
SL  Nucleus Position: SL-0198;
SL  Comments: Cytoplasm, cytosol {ECO:0000269|PubMed:12829261}. Nucleus {ECO:0000250|UniProtKB:P04797}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:12829261}. Membrane {ECO:0000269|PubMed:12829261}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P04797}. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). {ECO:0000250|UniProtKB:P04797, ECO:0000269|PubMed:12829261}.
DR  UNIPROT: P04406;
DR  UNIPROT: E7EUT4;
DR  UNIPROT: P00354;
DR  UNIPROT: Q53X65;
DR  PDB: 1U8F;
DR  PDB: 1ZNQ;
DR  PDB: 3GPD;
DR  PDB: 4WNC;
DR  PDB: 4WNI;
DR  PDB: 6ADE;
DR  PDB: 6IQ6;
DR  PDB: 6M61;
DR  PDB: 6YND;
DR  PDB: 6YNE;
DR  PDB: 6YNF;
DR  PDB: 6YNH;
DR  Pfam: PF02800;
DR  Pfam: PF00044;
DR  PROSITE: PS00071;
DR  OMIM: 138400;
DR  DisGeNET: 2597;
DE  Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D- glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1- dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). {ECO:0000250|UniProtKB:P04797, ECO:0000269|PubMed:11724794, ECO:0000269|PubMed:23071094, ECO:0000269|PubMed:23332158, ECO:0000269|PubMed:27387501, ECO:0000269|PubMed:3170585}.
DE  Reference Proteome: Yes;
DE  Interaction: P00533; IntAct: EBI-1188138; Score: 0.79
DE  Interaction: Q04864; IntAct: EBI-360834; Score: 0.00
DE  Interaction: Q99759; IntAct: EBI-362433; Score: 0.00
DE  Interaction: Q99558; IntAct: EBI-363130; Score: 0.00
DE  Interaction: O43353; IntAct: EBI-363706; Score: 0.00
DE  Interaction: Q9UHD2; IntAct: EBI-7476716; Score: 0.56
DE  Interaction: P20333; IntAct: EBI-364783; Score: 0.00
DE  Interaction: Q13077; IntAct: EBI-365002; Score: 0.00
DE  Interaction: P60709; IntAct: EBI-353790; Score: 0.40
DE  Interaction: Q13268; IntAct: EBI-354614; Score: 0.40
DE  Interaction: Q92993; IntAct: EBI-25857246; Score: 0.56
DE  Interaction: P15927; IntAct: EBI-707645; Score: 0.51
DE  Interaction: Q9NXU5; IntAct: EBI-728720; Score: 0.00
DE  Interaction: Q9BX70; IntAct: EBI-728775; Score: 0.00
DE  Interaction: Q16363; IntAct: EBI-729012; Score: 0.00
DE  Interaction: Q15102; IntAct: EBI-729096; Score: 0.00
DE  Interaction: Q9UN74; IntAct: EBI-729117; Score: 0.00
DE  Interaction: O00231; IntAct: EBI-729159; Score: 0.00
DE  Interaction: P50453; IntAct: EBI-729279; Score: 0.00
DE  Interaction: P04183; IntAct: EBI-7398156; Score: 0.55
DE  Interaction: O43504; IntAct: EBI-730105; Score: 0.00
DE  Interaction: Q06830; IntAct: EBI-730582; Score: 0.00
DE  Interaction: P08238; IntAct: EBI-709762; Score: 0.35
DE  Interaction: O60861; IntAct: EBI-7720940; Score: 0.40
DE  Interaction: O60739; IntAct: EBI-1072279; Score: 0.00
DE  Interaction: O00141; IntAct: EBI-1075257; Score: 0.00
DE  Interaction: Q9H0J4; IntAct: EBI-1078469; Score: 0.00
DE  Interaction: P10599; IntAct: EBI-25857222; Score: 0.68
DE  Interaction: P63104; IntAct: EBI-7194241; Score: 0.40
DE  Interaction: Q27957; IntAct: EBI-7025783; Score: 0.27
DE  Interaction: P01023; IntAct: EBI-7183126; Score: 0.35
DE  Interaction: Q14653; IntAct: EBI-7476716; Score: 0.35
DE  Interaction: P32121; IntAct: EBI-1642567; Score: 0.35
DE  Interaction: P42771; IntAct: EBI-1641665; Score: 0.35
DE  Interaction: P48637; IntAct: EBI-7830840; Score: 0.40
DE  Interaction: P28482; IntAct: EBI-2115126; Score: 0.00
DE  Interaction: O15264; IntAct: EBI-2255044; Score: 0.35
DE  Interaction: Q07820; IntAct: EBI-7173317; Score: 0.35
DE  Interaction: P00558; IntAct: EBI-7907378; Score: 0.54
DE  Interaction: P04406; IntAct: EBI-7907471; Score: 0.59
DE  Interaction: Q3KSU8; IntAct: EBI-2622922; Score: 0.37
DE  Interaction: P03372; IntAct: EBI-2877710; Score: 0.35
DE  Interaction: Q99459; IntAct: EBI-7954144; Score: 0.35
DE  Interaction: P12004; IntAct: EBI-8302829; Score: 0.57
DE  Interaction: Q9H492; IntAct: EBI-3044058; Score: 0.35
DE  Interaction: Q9GZQ8; IntAct: EBI-3045543; Score: 0.35
DE  Interaction: P60520; IntAct: EBI-3046676; Score: 0.35
DE  Interaction: Q9H0R8; IntAct: EBI-3048562; Score: 0.35
DE  Interaction: O95166; IntAct: EBI-3050465; Score: 0.35
DE  Interaction: P49768; IntAct: EBI-3385373; Score: 0.37
DE  Interaction: Q06187; IntAct: EBI-3505275; Score: 0.35
DE  Interaction: P43351; IntAct: EBI-3646827; Score: 0.35
DE  Interaction: Q96Q15; IntAct: EBI-3903995; Score: 0.35
DE  Interaction: Q15051; IntAct: EBI-4286917; Score: 0.35
DE  Interaction: P04083; IntAct: EBI-7095327; Score: 0.37
DE  Interaction: P20073; IntAct: EBI-7098273; Score: 0.37
DE  Interaction: P55957; IntAct: EBI-7104540; Score: 0.37
DE  Interaction: P24522; IntAct: EBI-7152962; Score: 0.37
DE  Interaction: P68032; IntAct: EBI-7157149; Score: 0.37
DE  Interaction: P00505; IntAct: EBI-7157215; Score: 0.37
DE  Interaction: Q14469; IntAct: EBI-7157494; Score: 0.37
DE  Interaction: Q15046; IntAct: EBI-7157574; Score: 0.37
DE  Interaction: Q13952; IntAct: EBI-7157626; Score: 0.37
DE  Interaction: Q99650; IntAct: EBI-7157758; Score: 0.37
DE  Interaction: Q92882; IntAct: EBI-7157808; Score: 0.37
DE  Interaction: P52756; IntAct: EBI-7157858; Score: 0.37
DE  Interaction: P62258; IntAct: EBI-7157955; Score: 0.37
DE  Interaction: P49841; IntAct: EBI-7165309; Score: 0.55
DE  Interaction: Q9Y6H6; IntAct: EBI-7183576; Score: 0.37
DE  Interaction: Q16637; IntAct: EBI-7389132; Score: 0.37
DE  Interaction: P37840; IntAct: EBI-7391160; Score: 0.37
DE  Interaction: P18084; IntAct: EBI-5659917; Score: 0.00
DE  Interaction: P15336; IntAct: EBI-5529812; Score: 0.35
DE  Interaction: P04487; IntAct: EBI-6157560; Score: 0.35
DE  Interaction: P0C1C6; IntAct: EBI-6158469; Score: 0.35
DE  Interaction: P03496; IntAct: EBI-6158649; Score: 0.35
DE  Interaction: P19320; IntAct: EBI-6189915; Score: 0.35
DE  Interaction: Q00537; IntAct: EBI-6380868; Score: 0.35
DE  Interaction: Q13164; IntAct: EBI-6380894; Score: 0.35
DE  Interaction: Q86VP6; IntAct: EBI-21322532; Score: 0.35
DE  Interaction: Q13616; IntAct: EBI-21323857; Score: 0.35
DE  Interaction: Q92905; IntAct: EBI-21325777; Score: 0.35
DE  Interaction: Q13617; IntAct: EBI-21327106; Score: 0.35
DE  Interaction: Q13620; IntAct: EBI-21327757; Score: 0.53
DE  Interaction: Q15843; IntAct: EBI-21328206; Score: 0.35
DE  Interaction: Q7L5N1; IntAct: EBI-21328549; Score: 0.35
DE  Interaction: Q13618; IntAct: EBI-21329068; Score: 0.35
DE  Interaction: Q9WMX2; IntAct: EBI-9078245; Score: 0.37
DE  Interaction: P42858; IntAct: EBI-9053301; Score: 0.67
DE  Interaction: P48147; IntAct: EBI-9550467; Score: 0.64
DE  Interaction: Q15303; IntAct: EBI-9687710; Score: 0.37
DE  Interaction: P04626; IntAct: EBI-9687897; Score: 0.55
DE  Interaction: Q60823; IntAct: EBI-9702520; Score: 0.42
DE  Interaction: Q13043; IntAct: EBI-10049589; Score: 0.35
DE  Interaction: P51451; IntAct: EBI-10102766; Score: 0.35
DE  Interaction: P05109; IntAct: EBI-10098112; Score: 0.65
DE  Interaction: P35228; IntAct: EBI-10091932; Score: 0.50
DE  Interaction: P06702; IntAct: EBI-10091932; Score: 0.35
DE  Interaction: Q00325; IntAct: EBI-10091892; Score: 0.35
DE  Interaction: P62805; IntAct: EBI-10091892; Score: 0.35
DE  Interaction: Q96FW1; IntAct: EBI-10770198; Score: 0.35
DE  Interaction: P19838; IntAct: EBI-11322719; Score: 0.35
DE  Interaction: P05412; IntAct: EBI-11323169; Score: 0.35
DE  Interaction: Q13098; IntAct: EBI-10766278; Score: 0.35
DE  Interaction: O35071; IntAct: EBI-10996866; Score: 0.35
DE  Interaction: P36895; IntAct: EBI-11008521; Score: 0.35
DE  Interaction: Q8N2N9; IntAct: EBI-11053871; Score: 0.35
DE  Interaction: Q96G25; IntAct: EBI-11053871; Score: 0.35
DE  Interaction: Q8NG31; IntAct: EBI-11053871; Score: 0.35
DE  Interaction: P48039; IntAct: EBI-11575898; Score: 0.37
DE  Interaction: P54274; IntAct: EBI-11310274; Score: 0.37
DE  Interaction: Q9BSI4; IntAct: EBI-11310284; Score: 0.51
DE  Interaction: Q96AP0; IntAct: EBI-11310294; Score: 0.37
DE  Interaction: Q9NUX5; IntAct: EBI-11310304; Score: 0.37
DE  Interaction: Q9UBX2; IntAct: EBI-11614356; Score: 0.35
DE  Interaction: P06929; IntAct: EBI-26506766; Score: 0.37
DE  Interaction: P06427; IntAct: EBI-26507442; Score: 0.37
DE  Interaction: P24835; IntAct: EBI-26507526; Score: 0.37
DE  Interaction: P56945; IntAct: EBI-15099384; Score: 0.35
DE  Interaction: Q6IQ23; IntAct: EBI-16398399; Score: 0.35
DE  Interaction: Q16082; IntAct: EBI-15487660; Score: 0.37
DE  Interaction: Q9BZE9; IntAct: EBI-21690090; Score: 0.35
DE  Interaction: O14556; IntAct: EBI-21690090; Score: 0.35
DE  Interaction: Q15915; IntAct: EBI-21696712; Score: 0.35
DE  Interaction: Q92696; IntAct: EBI-21736347; Score: 0.35
DE  Interaction: Q920M9; IntAct: EBI-15571980; Score: 0.44
DE  Interaction: Q7MWG4; IntAct: EBI-15591000; Score: 0.40
DE  Interaction: P16749; IntAct: EBI-15833083; Score: 0.41
DE  Interaction: P04075; IntAct: EBI-16426431; Score: 0.35
DE  Interaction: P00441; IntAct: EBI-20303881; Score: 0.50
DE  Interaction: P25705; IntAct: EBI-21929063; Score: 0.35
DE  Interaction: Q96HJ9; IntAct: EBI-21929593; Score: 0.35
DE  Interaction: Q9Y2Z9; IntAct: EBI-21930056; Score: 0.35
DE  Interaction: O00483; IntAct: EBI-21930681; Score: 0.35
DE  Interaction: O75489; IntAct: EBI-21931040; Score: 0.35
DE  Interaction: Q96FC7; IntAct: EBI-21931532; Score: 0.35
DE  Interaction: Q9H6K4; IntAct: EBI-21931410; Score: 0.35
DE  Interaction: Q3MIX3; IntAct: EBI-21935154; Score: 0.35
DE  Interaction: P0C7P0; IntAct: EBI-21935930; Score: 0.35
DE  Interaction: Q99807; IntAct: EBI-21936186; Score: 0.35
DE  Interaction: Q8TB22; IntAct: EBI-21937705; Score: 0.35
DE  Interaction: Q9NQH7; IntAct: EBI-21937834; Score: 0.35
DE  Interaction: Q9C002; IntAct: EBI-21938261; Score: 0.35
DE  Interaction: Q9HAC7; IntAct: EBI-21938493; Score: 0.35
DE  Interaction: O00746; IntAct: EBI-21940120; Score: 0.35
DE  Interaction: Q8N3Z0; IntAct: EBI-21940471; Score: 0.35
DE  Interaction: Q86U44; IntAct: EBI-20594935; Score: 0.35
DE  Interaction: Q9HCE5; IntAct: EBI-20595531; Score: 0.35
DE  Interaction: Q8TDU9; IntAct: EBI-20807086; Score: 0.37
DE  Interaction: P02730; IntAct: EBI-20795573; Score: 0.40
DE  Interaction: P04920; IntAct: EBI-20795563; Score: 0.40
DE  Interaction: P10636; IntAct: EBI-20799352; Score: 0.35
DE  Interaction: Q8NFJ5; IntAct: EBI-20902056; Score: 0.40
DE  Interaction: Q6A163; IntAct: EBI-20904168; Score: 0.40
DE  Interaction: O76041; IntAct: EBI-20904328; Score: 0.40
DE  Interaction: Q03936; IntAct: EBI-20906200; Score: 0.40
DE  Interaction: P04908; IntAct: EBI-20906576; Score: 0.40
DE  Interaction: Q5TZA2; IntAct: EBI-20909272; Score: 0.40
DE  Interaction: P57740; IntAct: EBI-20910640; Score: 0.40
DE  Interaction: Q99442; IntAct: EBI-20911552; Score: 0.40
DE  Interaction: O75602; IntAct: EBI-20914552; Score: 0.40
DE  Interaction: O95425; IntAct: EBI-20916064; Score: 0.40
DE  Interaction: Q5VST9; IntAct: EBI-20920604; Score: 0.40
DE  Interaction: Q502W7; IntAct: EBI-20925058; Score: 0.40
DE  Interaction: P48751; IntAct: EBI-20926210; Score: 0.40
DE  Interaction: Q00535; IntAct: EBI-20926978; Score: 0.40
DE  Interaction: A1A4G5; IntAct: EBI-20927136; Score: 0.40
DE  Interaction: Q69YH5; IntAct: EBI-20928976; Score: 0.40
DE  Interaction: Q13283; IntAct: EBI-20929856; Score: 0.40
DE  Interaction: Q63HK5; IntAct: EBI-20929960; Score: 0.40
DE  Interaction: Q5T6S3; IntAct: EBI-20931832; Score: 0.40
DE  Interaction: Q6P1J9; IntAct: EBI-20932880; Score: 0.40
DE  Interaction: O95139; IntAct: EBI-20934708; Score: 0.40
DE  Interaction: O15015; IntAct: EBI-20935244; Score: 0.40
DE  Interaction: A8MT65; IntAct: EBI-20937020; Score: 0.40
DE  Interaction: A2A935; IntAct: EBI-21022882; Score: 0.35
DE  Interaction: P14404; IntAct: EBI-21026252; Score: 0.35
DE  Interaction: O96013; IntAct: EBI-26962273; Score: 0.35
DE  Interaction: Q5T7N2; IntAct: EBI-20993270; Score: 0.46
DE  Interaction: P52292; IntAct: EBI-20993846; Score: 0.35
DE  Interaction: Q9H9Z2; IntAct: EBI-20993846; Score: 0.35
DE  Interaction: P13693; IntAct: EBI-20992046; Score: 0.35
DE  Interaction: Q5S007; IntAct: EBI-21360986; Score: 0.35
DE  Interaction: O60260; IntAct: EBI-21135687; Score: 0.35
DE  Interaction: P13569; IntAct: EBI-25416349; Score: 0.35
DE  Interaction: Q16526; IntAct: EBI-21981854; Score: 0.35
DE  Interaction: O95292; IntAct: EBI-21993601; Score: 0.35
DE  Interaction: P0DOF2; IntAct: EBI-25603126; Score: 0.35
DE  Interaction: P69479; IntAct: EBI-25568044; Score: 0.35
DE  Interaction: Q9BY14; IntAct: EBI-25504841; Score: 0.35
DE  Interaction: P0DTD1; IntAct: EBI-25509444; Score: 0.35
DE  Interaction: P0DTC3; IntAct: EBI-25510118; Score: 0.35
DE  Interaction: P0DTD3; IntAct: EBI-25510342; Score: 0.35
DE  Interaction: P17612; IntAct: EBI-25857214; Score: 0.56
DE  Interaction: Q9UIJ7; IntAct: EBI-25857278; Score: 0.56
DE  Interaction: Q8WUY3; IntAct: EBI-25857270; Score: 0.56
DE  Interaction: Q9UHX1; IntAct: EBI-25857262; Score: 0.56
DE  Interaction: O00471; IntAct: EBI-25857254; Score: 0.56
DE  Interaction: Q9BPW9; IntAct: EBI-25857238; Score: 0.56
DE  Interaction: O75344; IntAct: EBI-25857230; Score: 0.56
DE  Interaction: P06241; IntAct: EBI-25857206; Score: 0.56
DE  Interaction: P35222; IntAct: EBI-25857198; Score: 0.56
DE  Interaction: Q14194; IntAct: EBI-25857190; Score: 0.56
DE  Interaction: Q9UQM7; IntAct: EBI-25857182; Score: 0.56
DE  Interaction: Q6UY14; IntAct: EBI-25857286; Score: 0.56
DE  Interaction: Q96GZ6; IntAct: EBI-25857296; Score: 0.56
DE  Interaction: Q8NEA9; IntAct: EBI-25857304; Score: 0.56
DE  Interaction: P05067; IntAct: EBI-25936448; Score: 0.56
DE  Interaction: Q7Z417; IntAct: EBI-26513452; Score: 0.37
DE  Interaction: Q9BYB0; IntAct: EBI-26514195; Score: 0.37
DE  Interaction: P51531; IntAct: EBI-26515253; Score: 0.37
DE  Interaction: P49815; IntAct: EBI-26516083; Score: 0.37
DE  Interaction: P52298; IntAct: EBI-26397282; Score: 0.35
DE  Interaction: Q09161; IntAct: EBI-26399030; Score: 0.35
DE  Interaction: Q8TB24; IntAct: EBI-26518569; Score: 0.35
DE  Interaction: Q5U458; IntAct: EBI-26450034; Score: 0.35
DE  Interaction: P14240; IntAct: EBI-26968430; Score: 0.35
DE  Interaction: P60880; IntAct: EBI-26578093; Score: 0.35
DE  Interaction: Q99608; IntAct: EBI-26955247; Score: 0.27
DE  Interaction: P06401; IntAct: EBI-26871590; Score: 0.35
DE  Interaction: P35226; IntAct: EBI-27109494; Score: 0.35
DE  Interaction: Q92630; IntAct: EBI-28952196; Score: 0.27
DE  Interaction: P42695; IntAct: EBI-28951349; Score: 0.50
DE  Interaction: P0DTC9; IntAct: EBI-28955760; Score: 0.35
DE  Interaction: Q13619; IntAct: EBI-30863570; Score: 0.35
DE  Interaction: Q8WV16; IntAct: EBI-30863977; Score: 0.35
DE  Interaction: Q05DH4; IntAct: EBI-34574576; Score: 0.27
GO  GO:0005737;
GO  GO:0005829;
GO  GO:0070062;
GO  GO:0097452;
GO  GO:0043231;
GO  GO:0005811;
GO  GO:0016020;
GO  GO:0015630;
GO  GO:0031965;
GO  GO:0005634;
GO  GO:0048471;
GO  GO:0005886;
GO  GO:1990904;
GO  GO:0031982;
GO  GO:0019828;
GO  GO:0097718;
GO  GO:0004365;
GO  GO:0042802;
GO  GO:0008017;
GO  GO:0051287;
GO  GO:0050661;
GO  GO:0035605;
GO  GO:0061844;
GO  GO:0071346;
GO  GO:0050832;
GO  GO:0006006;
GO  GO:0006096;
GO  GO:0051873;
GO  GO:0031640;
GO  GO:0000226;
GO  GO:0010951;
GO  GO:0017148;
GO  GO:0051402;
GO  GO:0035606;
GO  GO:0001819;
GO  GO:0043123;
GO  GO:0032481;
GO  GO:0050821;
GO  GO:0016241;
TP  Membrane Topology: Unknown; Source: UniProt - Sequence Analysis;
SQ  MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQER
SQ  DPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPL
SQ  AKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANV
SQ  SVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGY
SQ  SNRVVDLMAHMASKE
//