ID  P33755;
PN  Nuclear protein localization protein 4;
GN  NPL4;
OS  559292;
SL  Nucleus Position: SL-0178;
SL  Nucleus Position: SL-0182;
SL  Nucleus Position: SL-0198;
SL  Comments: Cytoplasm, perinuclear region. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes mainly at the nuclear periphery and the endoplasmic reticulum membrane.
DR  UNIPROT: P33755;
DR  UNIPROT: D6VQG6;
DR  PDB: 6JWH;
DR  PDB: 6JWI;
DR  PDB: 6JWJ;
DR  PDB: 6OA9;
DR  PDB: 6OAA;
DR  Pfam: PF05021;
DR  Pfam: PF05020;
DR  PROSITE: PS50249;
DE  Function: Substrate-recruiting cofactor of the CDC48-NPL4-UFD1 segregase (PubMed:31249135). Assists CDC48 in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation (PubMed:11739805, PubMed:11740563, PubMed:11847109). Involved in the import of nuclear- targeted proteins into the nucleus and the export of poly(A) RNA out of the nucleus (PubMed:8930904, PubMed:11733065). Required for the proteasome-dependent processing/activation of MGA2 and SPT23 transcription factors leading to the subsequent expression of OLE1 (PubMed:11733065). Regulates ubiquitin-mediated mitochondria protein degradation (PubMed:31249135). Involved in spindle disassembly probably by promoting the degradation of spindle assemby factors ASE1 and CDC5 at the end of mitosis (PubMed:14636562). {ECO:0000269|PubMed:11733065, ECO:0000269|PubMed:11739805, ECO:0000269|PubMed:11740563, ECO:0000269|PubMed:11847109, ECO:0000269|PubMed:14636562, ECO:0000269|PubMed:31249135, ECO:0000269|PubMed:8930904}.
DE  Reference Proteome: Yes;
DE  Interaction: P53044; IntAct: EBI-390843; Score: 0.93
DE  Interaction: P32467; IntAct: EBI-792844; Score: 0.35
DE  Interaction: P16140; IntAct: EBI-794304; Score: 0.35
DE  Interaction: P02994; IntAct: EBI-794304; Score: 0.35
DE  Interaction: P06169; IntAct: EBI-794304; Score: 0.35
DE  Interaction: P25694; IntAct: EBI-794304; Score: 0.91
DE  Interaction: Q04228; IntAct: EBI-805595; Score: 0.74
DE  Interaction: P41832; IntAct: EBI-818477; Score: 0.27
DE  Interaction: Q02159; IntAct: EBI-860287; Score: 0.00
DE  Interaction: P39081; IntAct: EBI-861487; Score: 0.00
DE  Interaction: P28707; IntAct: EBI-7671928; Score: 0.40
DE  Interaction: P53899; IntAct: EBI-7872468; Score: 0.40
DE  Interaction: P47113; IntAct: EBI-8221329; Score: 0.22
DE  Interaction: Q05787; IntAct: EBI-1008463; Score: 0.35
DE  Interaction: Q08109; IntAct: EBI-1008463; Score: 0.53
DE  Interaction: P38307; IntAct: EBI-7894018; Score: 0.35
DE  Interaction: Q12743; IntAct: EBI-7894121; Score: 0.35
DE  Interaction: P54860; IntAct: EBI-2343183; Score: 0.55
DE  Interaction: Q05468; IntAct: EBI-6554533; Score: 0.35
DE  Interaction: Q12532; IntAct: EBI-6554563; Score: 0.35
DE  Interaction: Q04311; IntAct: EBI-9510937; Score: 0.40
DE  Interaction: Q03935; IntAct: EBI-16251296; Score: 0.00
DE  Interaction: P04838; IntAct: EBI-21915226; Score: 0.35
GO  GO:0036266;
GO  GO:0005737;
GO  GO:0000837;
GO  GO:0000839;
GO  GO:0031965;
GO  GO:0042175;
GO  GO:0005634;
GO  GO:0048471;
GO  GO:0030894;
GO  GO:1990112;
GO  GO:0034098;
GO  GO:0043130;
GO  GO:0031625;
GO  GO:0071629;
GO  GO:0006274;
GO  GO:0071712;
GO  GO:0072671;
GO  GO:0051228;
GO  GO:0051028;
GO  GO:0051974;
GO  GO:0070651;
GO  GO:1900182;
GO  GO:0043161;
GO  GO:0072665;
GO  GO:0006515;
GO  GO:0030970;
GO  GO:1990116;
GO  GO:0030433;
GO  GO:0006511;
TP  Membrane Topology: Peripheral; Source: UniProt - Sequence Analysis;
SQ  MLIRFRSKNGTHRVSCQENDLFGTVIEKLVGNLDPNADVDTFTVCEKPGQGIHAVSELADRTVMDLGLKHGDMLILNYSD
SQ  KPANEKDGVNVEIGSVGIDSKGIRQHRYGPLRIKELAVDEELEKEDGLIPRQKSKLCKHGDRGMCEYCSPLPPWDKEYHE
SQ  KNKIKHISFHSYLKKLNENANKKENGSSYISPLSEPDFRINKRCHNGHEPWPRGICSKCQPSAITLQQQEFRMVDHVEFQ
SQ  KSEIINEFIQAWRYTGMQRFGYMYGSYSKYDNTPLGIKAVVEAIYEPPQHDEQDGLTMDVEQVKNEMLQIDRQAQEMGLS
SQ  RIGLIFTDLSDAGAGDGSVFCKRHKDSFFLSSLEVIMAARHQTRHPNVSKYSEQGFFSSKFVTCVISGNLEGEIDISSYQ
SQ  VSTEAEALVTADMISGSTFPSMAYINDTTDERYVPEIFYMKSNEYGITVKENAKPAFPVDYLLVTLTHGFPNTDTETNSK
SQ  FVSSTGFPWSNRQAMGQSQDYQELKKYLFNVASSGDFNLLHEKISNFHLLLYINSLQILSPDEWKLLIESAVKNEWEESL
SQ  LKLVSSAGWQTLVMILQESG
//