ID  P40318;
PN  ERAD-associated E3 ubiquitin-protein ligase DOA10;
GN  SSM4;
OS  559292;
SL  Nucleus Position: SL-0178;
SL  Nucleus Position: SL-0179;
SL  Nucleus Position: SL-0182;
SL  Comments: Endoplasmic reticulum membrane {ECO:0000269|PubMed:11641273, ECO:0000269|PubMed:17051211}; Multi-pass membrane protein {ECO:0000255}. Nucleus inner membrane {ECO:0000269|PubMed:11641273, ECO:0000269|PubMed:17051211}; Multi-pass membrane protein {ECO:0000255}.
DR  UNIPROT: P40318;
DR  UNIPROT: D6VVQ1;
DR  PDB: 2M6M;
DR  Pfam: PF12906;
DR  PROSITE: PS51292;
DE  Function: E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC6 and UBC7 E2 ligases, and transfers it to substrates promoting their degradation (PubMed:11641273, PubMed:16179952, PubMed:16437165, PubMed:16873066, PubMed:17051211, PubMed:18812321, PubMed:20110468). Mediates the degradation of a broad range of substrates, including endoplasmic reticulum membrane proteins (ERQC), soluble nuclear proteins and soluble cytoplasmic proteins (CytoQC) (PubMed:11641273, PubMed:16179952, PubMed:16437165, PubMed:16873066, PubMed:17051211, PubMed:18812321, PubMed:20110468). Component of the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains (PubMed:16873066). ERAD-C substrates are ubiquitinated through DOA10 in conjunction with the E2 ubiquitin-conjugating enzymes UBC6 and UBC7-CUE1 (PubMed:11641273, PubMed:16179952, PubMed:16437165, PubMed:16873066, PubMed:17051211, PubMed:18812321, PubMed:20110468). Ubiquitinated substrates are then removed to the cytosol via the action of the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex and targeted to the proteasome (PubMed:11641273, PubMed:16179952, PubMed:16437165, PubMed:16873066, PubMed:17051211, PubMed:18812321, PubMed:20110468). Also recognizes the N-terminally acetylated residue of proteins as degradation signal (degron) (PubMed:20110468). N-terminally acetylated target proteins include MATALPHA2, TBF1, SLK19, YMR090W, HIS3, HSP104, UBP6 and ARO8 (PubMed:20110468). Catalyzes ubiquitination of mislocalized tail-anchored proteins that are extracted from the mitochondrion membrane by MSP1: following extraction, mistargeted proteins are transferred to the endoplasmic reticulum, where they are ubiquitinated by DOA10 and degraded by the proteasome (PubMed:31445887). {ECO:0000269|PubMed:11641273, ECO:0000269|PubMed:16179952, ECO:0000269|PubMed:16437165, ECO:0000269|PubMed:16873066, ECO:0000269|PubMed:17051211, ECO:0000269|PubMed:18812321, ECO:0000269|PubMed:20110468, ECO:0000269|PubMed:31445887}.
DE  Reference Proteome: Yes;
DE  Interaction: P39015; IntAct: EBI-939185; Score: 0.44
DE  Interaction: Q04121; IntAct: EBI-7756909; Score: 0.37
DE  Interaction: P47137; IntAct: EBI-939093; Score: 0.44
DE  Interaction: P40513; IntAct: EBI-939098; Score: 0.44
DE  Interaction: P06738; IntAct: EBI-939105; Score: 0.44
DE  Interaction: Q12007; IntAct: EBI-939110; Score: 0.44
DE  Interaction: P38758; IntAct: EBI-939115; Score: 0.44
DE  Interaction: P39683; IntAct: EBI-939120; Score: 0.44
DE  Interaction: P16467; IntAct: EBI-939125; Score: 0.44
DE  Interaction: P11154; IntAct: EBI-939130; Score: 0.44
DE  Interaction: P33399; IntAct: EBI-939135; Score: 0.44
DE  Interaction: P25617; IntAct: EBI-939140; Score: 0.44
DE  Interaction: P32288; IntAct: EBI-939145; Score: 0.44
DE  Interaction: P53982; IntAct: EBI-939150; Score: 0.44
DE  Interaction: P10963; IntAct: EBI-939155; Score: 0.44
DE  Interaction: P00925; IntAct: EBI-939160; Score: 0.44
DE  Interaction: P40825; IntAct: EBI-939165; Score: 0.44
DE  Interaction: P30952; IntAct: EBI-939170; Score: 0.44
DE  Interaction: Q04697; IntAct: EBI-939175; Score: 0.44
DE  Interaction: P32835; IntAct: EBI-939180; Score: 0.44
DE  Interaction: P23542; IntAct: EBI-939190; Score: 0.44
DE  Interaction: P38998; IntAct: EBI-939195; Score: 0.44
DE  Interaction: P38720; IntAct: EBI-939200; Score: 0.44
DE  Interaction: P35202; IntAct: EBI-939205; Score: 0.44
DE  Interaction: P80210; IntAct: EBI-939210; Score: 0.44
DE  Interaction: P40217; IntAct: EBI-939215; Score: 0.44
DE  Interaction: P38009; IntAct: EBI-939220; Score: 0.44
DE  Interaction: Q04458; IntAct: EBI-939225; Score: 0.44
DE  Interaction: P40959; IntAct: EBI-939230; Score: 0.44
DE  Interaction: Q05979; IntAct: EBI-939235; Score: 0.44
DE  Interaction: P40892; IntAct: EBI-939240; Score: 0.44
DE  Interaction: P08524; IntAct: EBI-939245; Score: 0.44
DE  Interaction: P25561; IntAct: EBI-939250; Score: 0.44
DE  Interaction: P38787; IntAct: EBI-939255; Score: 0.44
DE  Interaction: P46655; IntAct: EBI-939260; Score: 0.44
DE  Interaction: P38158; IntAct: EBI-939265; Score: 0.44
DE  Interaction: P38081; IntAct: EBI-939270; Score: 0.44
DE  Interaction: Q01454; IntAct: EBI-939275; Score: 0.44
DE  Interaction: Q08968; IntAct: EBI-939280; Score: 0.44
DE  Interaction: P41816; IntAct: EBI-939285; Score: 0.44
DE  Interaction: P46151; IntAct: EBI-939290; Score: 0.44
DE  Interaction: P54885; IntAct: EBI-939295; Score: 0.44
DE  Interaction: Q08686; IntAct: EBI-939300; Score: 0.44
DE  Interaction: Q08444; IntAct: EBI-939305; Score: 0.44
DE  Interaction: P50278; IntAct: EBI-939310; Score: 0.44
DE  Interaction: P37254; IntAct: EBI-939315; Score: 0.44
DE  Interaction: Q10740; IntAct: EBI-939320; Score: 0.44
DE  Interaction: P50101; IntAct: EBI-939325; Score: 0.44
DE  Interaction: P00331; IntAct: EBI-939330; Score: 0.44
DE  Interaction: P16862; IntAct: EBI-939335; Score: 0.44
DE  Interaction: P46680; IntAct: EBI-939340; Score: 0.44
DE  Interaction: Q04212; IntAct: EBI-939345; Score: 0.44
DE  Interaction: P15274; IntAct: EBI-939350; Score: 0.44
DE  Interaction: P54007; IntAct: EBI-939355; Score: 0.44
DE  Interaction: Q99260; IntAct: EBI-939360; Score: 0.44
DE  Interaction: Q04491; IntAct: EBI-939365; Score: 0.44
DE  Interaction: P31539; IntAct: EBI-939370; Score: 0.44
DE  Interaction: P10592; IntAct: EBI-939375; Score: 0.44
DE  Interaction: P41903; IntAct: EBI-939380; Score: 0.44
DE  Interaction: P38716; IntAct: EBI-939385; Score: 0.44
DE  Interaction: P17076; IntAct: EBI-939390; Score: 0.44
DE  Interaction: P53051; IntAct: EBI-939395; Score: 0.44
DE  Interaction: P53265; IntAct: EBI-939400; Score: 0.44
DE  Interaction: P53101; IntAct: EBI-939405; Score: 0.44
DE  Interaction: P53183; IntAct: EBI-939410; Score: 0.44
DE  Interaction: P10356; IntAct: EBI-939415; Score: 0.44
DE  Interaction: P21524; IntAct: EBI-939420; Score: 0.44
DE  Interaction: P25044; IntAct: EBI-939425; Score: 0.44
DE  Interaction: Q07505; IntAct: EBI-939430; Score: 0.44
DE  Interaction: P16120; IntAct: EBI-939435; Score: 0.44
DE  Interaction: P25576; IntAct: EBI-939440; Score: 0.44
DE  Interaction: P17967; IntAct: EBI-939445; Score: 0.44
DE  Interaction: P00817; IntAct: EBI-939450; Score: 0.44
DE  Interaction: Q04228; IntAct: EBI-982121; Score: 0.71
DE  Interaction: P38428; IntAct: EBI-982234; Score: 0.56
DE  Interaction: P38911; IntAct: EBI-982234; Score: 0.35
DE  Interaction: P25694; IntAct: EBI-982234; Score: 0.71
DE  Interaction: P33296; IntAct: EBI-1102319; Score: 0.40
DE  Interaction: Q02159; IntAct: EBI-1102333; Score: 0.40
DE  Interaction: P53044; IntAct: EBI-1008654; Score: 0.46
DE  Interaction: P12866; IntAct: EBI-1637277; Score: 0.64
DE  Interaction: P32589; IntAct: EBI-3807868; Score: 0.35
DE  Interaction: P38360; IntAct: EBI-15787591; Score: 0.40
DE  Interaction: P53756; IntAct: EBI-20814404; Score: 0.37
GO  GO:0000837;
GO  GO:0005783;
GO  GO:0030176;
GO  GO:0016021;
GO  GO:0005635;
GO  GO:0005637;
GO  GO:0061630;
GO  GO:0004842;
GO  GO:0008270;
GO  GO:0071712;
GO  GO:0030970;
GO  GO:0030433;
TP  Membrane Topology: Transmembrane; Source: UniProt - Sequence Analysis {ECO:0000255};
SQ  MDVDSDVNVSRLRDELHKVANEETDTATFNDDAPSGATCRICRGEATEDNPLFHPCKCRGSIKYMHESCLLEWVASKNID
SQ  ISKPGADVKCDICHYPIQFKTIYAENMPEKIPFSLLLSKSILTFFEKARLALTIGLAAVLYIIGVPLVWNMFGKLYTMML
SQ  DGSSPYPGDFLKSLIYGYDQSATPELTTRAIFYQLLQNHSFTSLQFIMIVILHIALYFQYDMIVREDVFSKMVFHKIGPR
SQ  LSPKDLKSRLKERFPMMDDRMVEYLAREMRAHDENRQEQGHDRLNMPAAAADNNNNVINPRNDNVPPQDPNDHRNFENLR
SQ  HVDELDHDEATEEHENNDSDNSLPSGDDSSRILPGSSSDNEEDEEAEGQQQQQQPEEEADYRDHIEPNPIDMWANRRAQN
SQ  EFDDLIAAQQNAINRPNAPVFIPPPAQNRAGNVDQDEQDFGAAVGVPPAQANPDDQGQGPLVINLKLKLLNVIAYFIIAV
SQ  VFTAIYLAISYLFPTFIGFGLLKIYFGIFKVILRGLCHLYYLSGAHIAYNGLTKLVPKVDVAMSWISDHLIHDIIYLYNG
SQ  YTENTMKHSIFIRALPALTTYLTSVSIVCASSNLVSRGYGRENGMSNPTRRLIFQILFALKCTFKVFTLFFIELAGFPIL
SQ  AGVMLDFSLFCPILASNSRMLWVPSICAIWPPFSLFVYWTIGTLYMYWFAKYIGMIRKNIIRPGVLFFIRSPEDPNIKIL
SQ  HDSLIHPMSIQLSRLCLSMFIYAIFIVLGFGFHTRIFFPFMLKSNLLSVPEAYKPTSIISWKFNTILLTLYFTKRILESS
SQ  SYVKPLLERYWKTIFKLCSRKLRLSSFILGKDTPTERGHIVYRNLFYKYIAAKNAEWSNQELFTKPKTLEQAEELFGQVR
SQ  DVHAYFVPDGVLMRVPSSDIVSRNYVQTMFVPVTKDDKLLKPLDLERIKERNKRAAGEFGYLDEQNTEYDQYYIVYVPPD
SQ  FRLRYMTLLGLVWLFASILMLGVTFISQALINFVCSFGFLPVVKLLLGERNKVYVAWKELSDISYSYLNIYYVCVGSVCL
SQ  SKIAKDILHFTEGQNTLDEHAVDENEVEEVEHDIPERDINNAPVNNINNVEEGQGIFMAIFNSIFDSMLVKYNLMVFIAI
SQ  MIAVIRTMVSWVVLTDGILACYNYLTIRVFGNSSYTIGNSKWFKYDESLLFVVWIISSMVNFGTGYKSLKLFFRNRNTSK
SQ  LNFLKTMALELFKQGFLHMVIYVLPIIILSLVFLRDVSTKQIIDISHGSRSFTLSLNESFPTWTRMQDIYFGLLIALESF
SQ  TFFFQATVLFIQWFKSTVQNVKDEVYTKGRALENLPDES
//