ID  P53983;
PN  Probable ERAD-associated E3 ubiquitin-protein ligase ASI1;
GN  ASI3;
OS  559292;
SL  Nucleus Position: SL-0178;
SL  Nucleus Position: SL-0179;
SL  Nucleus Position: SL-0182;
SL  Comments: Nucleus inner membrane {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:17085444}; Multi-pass membrane protein {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:17085444}.
DR  UNIPROT: P53983;
DR  UNIPROT: D6W1G9;
DE  Function: Part of the nuclear inner membrane (INM)-specific branch of the ER-associated degradation (ERAD) pathway, required for the elimination of misfolded proteins in the INM, a specialized ER subdomain. Required for ERG11 degradation (PubMed:25236469). Negative regulator of SPS-sensor signaling. Together with ASI2 and ASI3, prevents the unprocessed precursor forms of STP1 and STP2 that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes in the absence of inducing signals (PubMed:17085444). Controls amino acid permease (AAP) gene expression in response to amino acid availability, a process mediated by the transcription factors STP1 and STP1 (PubMed:11454748). {ECO:0000269|PubMed:11454748, ECO:0000269|PubMed:17085444, ECO:0000269|PubMed:25236469}.
DE  Reference Proteome: Yes;
DE  Interaction: P25491; IntAct: EBI-3763707; Score: 0.35
DE  Interaction: P53895; IntAct: EBI-10096480; Score: 0.46
DE  Interaction: P25346; IntAct: EBI-7752898; Score: 0.37
DE  Interaction: P38156; IntAct: EBI-7752951; Score: 0.37
DE  Interaction: P49626; IntAct: EBI-787273; Score: 0.35
DE  Interaction: P0C2I0; IntAct: EBI-787273; Score: 0.35
DE  Interaction: Q02159; IntAct: EBI-860284; Score: 0.00
DE  Interaction: P25294; IntAct: EBI-3660235; Score: 0.35
DE  Interaction: P10591; IntAct: EBI-3667430; Score: 0.35
DE  Interaction: P11484; IntAct: EBI-3777888; Score: 0.35
DE  Interaction: P39077; IntAct: EBI-3824502; Score: 0.35
DE  Interaction: P54074; IntAct: EBI-10096480; Score: 0.56
DE  Interaction: P10614; IntAct: EBI-10096429; Score: 0.40
DE  Interaction: P53846; IntAct: EBI-16291965; Score: 0.35
GO  GO:0097658;
GO  GO:0016021;
GO  GO:0005637;
GO  GO:0034399;
GO  GO:0046872;
GO  GO:0004842;
GO  GO:0071230;
GO  GO:0036369;
GO  GO:0006511;
TP  Membrane Topology: Transmembrane; Source: UniProt - Sequence Analysis {ECO:0000255};
SQ  MSTNILQHVKQLLHNRDVFSFFHNKTGNLNYLDNTTQKPEVFVSPNSTIVSAPTLDSFQALMEKGNFTTLQLAKVGIRMF
SQ  FSYSVSKYAVLCFSTAIILNRLTVMSSLRSNSTNIRLPLWSKTLLHLVATLSLVKALLQILSQFGLMHELHVSDTDFYAL
SQ  SVYLFVALSDCIEIFISSTTNVPSLICSDFSIWGLSLNLYIISKMPAGQQHIGDNVELLGAVFHRLVIHLVELFHIRAYR
SQ  LCGEVILNAGFFTAFVTRTYLNGLDFINICLIHNYFPGFFYISTILLASIGIFLKALFTSNPFRSLYSRYKNLEKWWRSN
SQ  NYNGEEEFNEIALSLCLLLTSNDYKIFKKSDNVKSVDEVAAFSNSYVVSGHLNQLQSTPEDLLSRKEMTTDSQLPGFART
SQ  YLGLFELVRTIILTYSRLLKNLLWSKNFESSIDKKPRVGKRKKRDLNKYVTEKNYKKFLYKPDVKELNIESDLRSLELLL
SQ  PEDDSSKDYFPPRKIDESVSDEEFDSDMESQLIIDEEKELTHLSSNAVDSDDLEEIAWNISMWSILNYEMDVHNKVNGPL
SQ  TRSQYGKRNPQGVLVDVVIERLLHHTNSRYMYKRLNMKDDDKLEFKFDFAFDSCDEVEEMDLSCLICKVNKRNIVTWPCR
SQ  CLALCDDCRISLGYKGFATCVSCDSEVKGYSKLNIV
//