ID  Q08AE8;
PN  Protein spire homolog 1;
GN  SPIRE1;
OS  9606;
SL  Nucleus Position: SL-0198;
SL  Comments: Cytoplasm, cytoskeleton {ECO:0000269|PubMed:11747823}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:11747823}. Cell membrane {ECO:0000269|PubMed:11747823}; Peripheral membrane protein {ECO:0000269|PubMed:11747823}; Cytoplasmic side {ECO:0000269|PubMed:11747823}. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q52KF3}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q52KF3}; Cytoplasmic side {ECO:0000250|UniProtKB:Q52KF3}. Note=Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion (By similarity). Punctate spots in perinuclear region and cytoplasm, colocalized with Rab11 (By similarity). {ECO:0000250|UniProtKB:Q52KF3}.
DR  UNIPROT: Q08AE8;
DR  UNIPROT: A8K2B5;
DR  UNIPROT: J3KQ50;
DR  UNIPROT: J3KQR5;
DR  UNIPROT: Q1RMD4;
DR  UNIPROT: Q8NDP1;
DR  UNIPROT: Q9NQ71;
DR  UNIPROT: Q9ULT4;
DR  PDB: 2YLE;
DR  PDB: 2YLF;
DR  PDB: 3R7G;
DR  PDB: 3RBW;
DR  Pfam: PF16474;
DR  PROSITE: PS51377;
DR  OMIM: 609216;
DR  DisGeNET: 56907;
DE  Function: Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:11747823, PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed:11747823). Required for asymmetric spindle positioning and asymmetric cell division during meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with FMN2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed:26287480). In addition, promotes innate immune signaling downstream of dsRNA sensing (PubMed:35148361). Mechanistically, contributes to IRF3 phosphorylation and activation downstream of MAVS and upstream of TBK1 (PubMed:35148361). {ECO:0000269|PubMed:11747823, ECO:0000269|PubMed:21620703, ECO:0000269|PubMed:26287480, ECO:0000269|PubMed:35148361}.
DE  Reference Proteome: Yes;
DE  Interaction: P27348; IntAct: EBI-21902679; Score: 0.56
DE  Interaction: P31947; IntAct: EBI-7544015; Score: 0.40
DE  Interaction: P68466; IntAct: EBI-6158939; Score: 0.35
DE  Interaction: O75161; IntAct: EBI-11783022; Score: 0.40
DE  Interaction: Q96RK4; IntAct: EBI-24711859; Score: 0.56
DE  Interaction: Q9Y3M2; IntAct: EBI-11911975; Score: 0.00
DE  Interaction: P56945; IntAct: EBI-15099538; Score: 0.35
DE  Interaction: Q4VC05; IntAct: EBI-21637103; Score: 0.35
DE  Interaction: Q8WUZ0; IntAct: EBI-21637520; Score: 0.35
DE  Interaction: Q9NYL9; IntAct: EBI-21701240; Score: 0.35
DE  Interaction: P31946; IntAct: EBI-21903886; Score: 0.35
DE  Interaction: P61981; IntAct: EBI-21905461; Score: 0.35
DE  Interaction: Q04917; IntAct: EBI-21907689; Score: 0.35
DE  Interaction: P63104; IntAct: EBI-21909838; Score: 0.35
DE  Interaction: P68135; IntAct: EBI-16095106; Score: 0.44
DE  Interaction: P03372; IntAct: EBI-21301141; Score: 0.35
DE  Interaction: P13637; IntAct: EBI-25832773; Score: 0.56
DE  Interaction: P16284; IntAct: EBI-25881511; Score: 0.56
DE  Interaction: Q13393; IntAct: EBI-25882505; Score: 0.56
GO  GO:0005938;
GO  GO:0030659;
GO  GO:0005856;
GO  GO:0005829;
GO  GO:0031307;
GO  GO:0005654;
GO  GO:0048471;
GO  GO:0005886;
GO  GO:0003779;
GO  GO:0030036;
GO  GO:0051639;
GO  GO:0030041;
GO  GO:0045010;
GO  GO:0036089;
GO  GO:0051295;
GO  GO:0070649;
GO  GO:0048193;
GO  GO:0046907;
GO  GO:0040038;
GO  GO:2000781;
GO  GO:0090141;
GO  GO:0015031;
GO  GO:0016192;
TP  Membrane Topology: Peripheral; Source: UniProt - By Similarity {ECO:0000250|UniProtKB:Q52KF3};
SQ  MAQAAGPAGGGEPRTEAVGGEGPREPGAAGGAAGGSRDALSLEEILRLYNQPINEEQAWAVCYQCCGSLRAAARRRQPRH
SQ  RVRSAAQIRVWRDGAVTLAPAADDAGEPPPVAGKLGYSQCMETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDHM
SQ  ANTVEADGSNDEGYEAAEEGLGDEDEKRKISAIRSYRDVMKLCAAHLPTESDAPNHYQAVCRALFAETMELHTFLTKIKS
SQ  AKENLKKIQEMEKSDESSTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRCKRYTL
SQ  RKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPVSARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLAMRP
SQ  LSMSYSFDLSDVTTPESTKNLVESSMVNGGLTSQTKENGLSTSQQVPAQRKKLLRAPTLAELDSSESEEETLHKSTSSSS
SQ  VSPSFPEEPVLEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSRQSSRSLEEFCYPVECLALTV
SQ  EEVMHIRQVLVKAELEKYQQYKDIYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFS
SQ  LGPSALQRGESSMRSEKPSTAHHRPLRSIARFSSKSKSMDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSL
SQ  VLANKRARLKRKTQSFYMSSPGPSEYCPSERTISEI
//