ID  Q15811;
PN  Intersectin-1;
GN  ITSN1;
OS  9606;
SL  Nucleus Position: SL-0178;
SL  Comments: Endomembrane system {ECO:0000269|PubMed:11744688}. Synapse, synaptosome {ECO:0000250|UniProtKB:Q9WVE9}. Cell projection, lamellipodium {ECO:0000269|PubMed:11744688}. Cell membrane {ECO:0000269|PubMed:11744688, ECO:0000269|PubMed:20946875}. Membrane, clathrin-coated pit {ECO:0000269|PubMed:20946875, ECO:0000269|PubMed:29887380}. Recycling endosome {ECO:0000269|PubMed:29030480}. Endosome {ECO:0000250|UniProtKB:Q9Z0R4}. Cytoplasmic vesicle {ECO:0000250|UniProtKB:Q9Z0R4}. Note=Colocalizes with SGIP1 at the plasma membrane in structures corresponding most probably to clathrin-coated pits (PubMed:20946875). Colocalizes with RAB13 on cytoplasmic vesicles that are most likely recycling endosomes (PubMed:29030480). {ECO:0000269|PubMed:20946875, ECO:0000269|PubMed:29030480}. [Isoform 2]: Cytoplasm {ECO:0000269|PubMed:29599122}. Endomembrane system {ECO:0000269|PubMed:21712076}. Nucleus envelope {ECO:0000269|PubMed:29599122}. Note=Shuttles between the cytoplasm and nucleus in an XPO1/CRM1-dependent manner. {ECO:0000269|PubMed:29599122}. [Isoform 5]: Endomembrane system {ECO:0000269|PubMed:21712076}.
DR  UNIPROT: Q15811;
DR  UNIPROT: A7Y322;
DR  UNIPROT: A8CTX8;
DR  UNIPROT: A8CTY3;
DR  UNIPROT: A8CTY7;
DR  UNIPROT: A8D7D0;
DR  UNIPROT: A8DCP3;
DR  UNIPROT: B4DTM2;
DR  UNIPROT: E7ERJ1;
DR  UNIPROT: E9PE44;
DR  UNIPROT: E9PG01;
DR  UNIPROT: E9PHV2;
DR  UNIPROT: O95216;
DR  UNIPROT: Q0PW94;
DR  UNIPROT: Q0PW95;
DR  UNIPROT: Q0PW97;
DR  UNIPROT: Q14BD3;
DR  UNIPROT: Q1ED40;
DR  UNIPROT: Q20BK3;
DR  UNIPROT: Q9UET5;
DR  UNIPROT: Q9UK60;
DR  UNIPROT: Q9UNK1;
DR  UNIPROT: Q9UNK2;
DR  UNIPROT: Q9UQ92;
DR  PDB: 1KI1;
DR  PDB: 2KGR;
DR  PDB: 2KHN;
DR  PDB: 3FIA;
DR  PDB: 3QBV;
DR  PDB: 4IIM;
DR  PDB: 5HZI;
DR  PDB: 5HZJ;
DR  PDB: 5HZK;
DR  PDB: 6GBU;
DR  PDB: 6H5T;
DR  Pfam: PF00168;
DR  Pfam: PF12763;
DR  Pfam: PF16617;
DR  Pfam: PF16652;
DR  Pfam: PF00621;
DR  Pfam: PF00018;
DR  Pfam: PF07653;
DR  Pfam: PF14604;
DR  PROSITE: PS50004;
DR  PROSITE: PS00741;
DR  PROSITE: PS50010;
DR  PROSITE: PS00018;
DR  PROSITE: PS50222;
DR  PROSITE: PS50031;
DR  PROSITE: PS50003;
DR  PROSITE: PS50002;
DR  OMIM: 602442;
DR  DisGeNET: 6453;
DE  Function: Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery (PubMed:11584276, PubMed:29887380). Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (PubMed:11584276). Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity). Recruits FCHSD2 to clathrin-coated pits (PubMed:29887380). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (By similarity). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (PubMed:22648170). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (By similarity). Inhibits ARHGAP31 activity toward RAC1 (PubMed:11744688). {ECO:0000250|UniProtKB:Q9WVE9, ECO:0000250|UniProtKB:Q9Z0R4, ECO:0000269|PubMed:11584276, ECO:0000269|PubMed:11744688, ECO:0000269|PubMed:22648170, ECO:0000269|PubMed:29887380}. [Isoform 1]: Plays a role in synaptic vesicle endocytosis in brain neurons. {ECO:0000250|UniProtKB:Q9Z0R4}.
DE  Reference Proteome: Yes;
DE  Interaction: O43426; IntAct: EBI-7965393; Score: 0.40
DE  Interaction: P00533; IntAct: EBI-32720286; Score: 0.27
DE  Interaction: P09917; IntAct: EBI-21805075; Score: 0.35
DE  Interaction: P35658; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: P60880; IntAct: EBI-8589666; Score: 0.35
DE  Interaction: Q12840; IntAct: EBI-730237; Score: 0.00
DE  Interaction: P42566; IntAct: EBI-6951101; Score: 0.56
DE  Interaction: Q07889; IntAct: EBI-7222790; Score: 0.64
DE  Interaction: O00401; IntAct: EBI-7987685; Score: 0.70
DE  Interaction: P60953; IntAct: EBI-602095; Score: 0.54
DE  Interaction: P61968; IntAct: EBI-7222047; Score: 0.37
DE  Interaction: Q05193; IntAct: EBI-7591434; Score: 0.62
DE  Interaction: P50570; IntAct: EBI-7591943; Score: 0.71
DE  Interaction: P68104; IntAct: EBI-730234; Score: 0.00
DE  Interaction: Q13432; IntAct: EBI-730240; Score: 0.00
DE  Interaction: Q9BYC9; IntAct: EBI-732259; Score: 0.00
DE  Interaction: Q9Y5X3; IntAct: EBI-732262; Score: 0.00
DE  Interaction: Q8TAQ2; IntAct: EBI-734884; Score: 0.00
DE  Interaction: Q9NRI5; IntAct: EBI-928807; Score: 0.55
DE  Interaction: P09172; IntAct: EBI-8589642; Score: 0.27
DE  Interaction: Q15811; IntAct: EBI-8589666; Score: 0.35
DE  Interaction: O75940; IntAct: EBI-1068586; Score: 0.00
DE  Interaction: O15357; IntAct: EBI-8052389; Score: 0.60
DE  Interaction: P22681; IntAct: EBI-8611246; Score: 0.73
DE  Interaction: Q925Q9; IntAct: EBI-8555189; Score: 0.52
DE  Interaction: P04370; IntAct: EBI-7728309; Score: 0.40
DE  Interaction: P39053; IntAct: EBI-7965349; Score: 0.40
DE  Interaction: Q2M1Z3; IntAct: EBI-7965368; Score: 0.40
DE  Interaction: P22682; IntAct: EBI-7965471; Score: 0.40
DE  Interaction: Q62245; IntAct: EBI-7965553; Score: 0.40
DE  Interaction: Q3TTA7; IntAct: EBI-7965508; Score: 0.40
DE  Interaction: P63010; IntAct: EBI-30816498; Score: 0.59
DE  Interaction: O43150; IntAct: EBI-2654148; Score: 0.00
DE  Interaction: Q6ZUJ8; IntAct: EBI-2654176; Score: 0.00
DE  Interaction: Q15427; IntAct: EBI-2654196; Score: 0.00
DE  Interaction: P22674; IntAct: EBI-2654206; Score: 0.00
DE  Interaction: Q99471; IntAct: EBI-2654186; Score: 0.00
DE  Interaction: Q9Y2W2; IntAct: EBI-2654216; Score: 0.00
DE  Interaction: A0A6H3A910; IntAct: EBI-2831328; Score: 0.00
DE  Interaction: P67870; IntAct: EBI-7133985; Score: 0.37
DE  Interaction: Q16659; IntAct: EBI-7210088; Score: 0.37
DE  Interaction: Q13387; IntAct: EBI-7238306; Score: 0.37
DE  Interaction: P53350; IntAct: EBI-7313057; Score: 0.37
DE  Interaction: O75582; IntAct: EBI-7373257; Score: 0.37
DE  Interaction: Q99962; IntAct: EBI-7387564; Score: 0.37
DE  Interaction: P04183; IntAct: EBI-7398611; Score: 0.37
DE  Interaction: P61086; IntAct: EBI-7411905; Score: 0.37
DE  Interaction: Q9NYL2; IntAct: EBI-7418184; Score: 0.37
DE  Interaction: Q3UQN2; IntAct: EBI-6097080; Score: 0.56
DE  Interaction: O43175; IntAct: EBI-9395254; Score: 0.35
DE  Interaction: Q92734; IntAct: EBI-11029015; Score: 0.35
DE  Interaction: Q9NS91; IntAct: EBI-11143700; Score: 0.35
DE  Interaction: Q96CW1; IntAct: EBI-21520588; Score: 0.35
DE  Interaction: Q8NB37; IntAct: EBI-21611026; Score: 0.35
DE  Interaction: Q9NZQ3; IntAct: EBI-21676174; Score: 0.35
DE  Interaction: Q9NVZ3; IntAct: EBI-21720899; Score: 0.35
DE  Interaction: Q8N9I9; IntAct: EBI-21791883; Score: 0.35
DE  Interaction: Q59EK9; IntAct: EBI-21795241; Score: 0.35
DE  Interaction: P53680; IntAct: EBI-21808127; Score: 0.35
DE  Interaction: Q86X29; IntAct: EBI-21808254; Score: 0.35
DE  Interaction: Q9HCN6; IntAct: EBI-15816584; Score: 0.40
DE  Interaction: O43493; IntAct: EBI-16800265; Score: 0.27
DE  Interaction: P15311; IntAct: EBI-16792219; Score: 0.27
DE  Interaction: Q13444; IntAct: EBI-21226632; Score: 0.44
DE  Interaction: O43184; IntAct: EBI-21227583; Score: 0.44
DE  Interaction: Q6NSK7; IntAct: EBI-21386219; Score: 0.00
DE  Interaction: Q15599; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: P22307; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: O95757; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: P51659; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: Q9UBC2; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: Q13011; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: P09496; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: Q969X6; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: P04040; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: O94973; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: O95782; IntAct: EBI-25410025; Score: 0.35
DE  Interaction: Q9BYB0; IntAct: EBI-26514359; Score: 0.37
DE  Interaction: Q9BZD6; IntAct: EBI-26604363; Score: 0.44
DE  Interaction: P07355; IntAct: EBI-27094310; Score: 0.52
DE  Interaction: P11362; IntAct: EBI-32721578; Score: 0.27
DE  Interaction: P22455; IntAct: EBI-32722168; Score: 0.27
DE  Interaction: Q6ZMQ8; IntAct: EBI-32723474; Score: 0.27
GO  GO:0070161;
GO  GO:0005905;
GO  GO:0005737;
GO  GO:0005829;
GO  GO:0030027;
GO  GO:0043005;
GO  GO:0005635;
GO  GO:0005886;
GO  GO:0098793;
GO  GO:0055037;
GO  GO:0005509;
GO  GO:0005085;
GO  GO:0060090;
GO  GO:0070064;
GO  GO:0150007;
GO  GO:0006897;
GO  GO:0016197;
GO  GO:0006887;
GO  GO:0035556;
GO  GO:0008104;
GO  GO:0015031;
GO  GO:0051056;
TP  Membrane Topology: Unknown; Source: UniProt - Sequence Analysis;
SQ  MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSI
SQ  AMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLAN
SQ  GAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHL
SQ  TGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVI
SQ  SSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQE
SQ  QERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEF
SQ  ELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSL
SQ  HRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKII
SQ  ELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAV
SQ  QAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANY
SQ  AEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLT
SQ  VPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGW
SQ  FPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWT
SQ  GTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARG
SQ  KKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQ
SQ  VGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAM
SQ  IFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRL
SQ  AMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHV
SQ  QCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPI
SQ  FLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMV
SQ  NVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEI
SQ  RVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP
//