ID  Q76L40;
PN  RNA-directed RNA polymerase;
GN  RNA1;
OS  649895;
SL  Nucleus Position: SL-0382;
SL  Comments: [Putative helicase]: Host membrane {ECO:0000250|UniProtKB:P03600}; Single-pass membrane protein {ECO:0000250|UniProtKB:P03600}. Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:P03600}. [RNA-directed RNA polymerase]: Host endoplasmic reticulum {ECO:0000250|UniProtKB:P03600}. [Protease cofactor]: Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:P03600}.
DR  UNIPROT: Q76L40;
DR  Pfam: PF00548;
DR  Pfam: PF00680;
DR  Pfam: PF00910;
DR  PROSITE: PS51874;
DR  PROSITE: PS50507;
DR  PROSITE: PS51218;
DE  Function: [Picornain 3C-like protease]: Thiol protease that cleaves the RNA1 and RNA2 polyproteins. {ECO:0000250|UniProtKB:P03600}. [Viral genome-linked protein]: Plays a role in RNA replication. It is covalently linked to the 5'terminus of both viral single-stranded RNA1 and RNA2 molecules. {ECO:0000250|UniProtKB:P03600}. [Protease cofactor]: Down-regulates the RNA1 polyprotein processing and enhances trans-cleavage of RNA2 polyproteins. The protease cofactor and the putative helicase seem to target the replication complexes to ER membranes. Their physical association causes the membrane rearrangement of host ER that may result in formation of the small membranous vesicles that are the site of viral RNA synthesis. {ECO:0000250|UniProtKB:P03600}. [Putative helicase]: The protease cofactor and the putative helicase seem to target the replication complexes to ER membranes. Their physical association causes the membrane rearrangement of host ER that may result in formation of the small membranous vesicles that are the site of viral RNA synthesis. {ECO:0000250|UniProtKB:P03600}. [RNA-directed RNA polymerase]: Replicates the viral genome. {ECO:0000250|UniProtKB:P03600}.
DE  Reference Proteome: Yes;
GO  GO:0044165;
GO  GO:0033644;
GO  GO:0044220;
GO  GO:0016021;
GO  GO:0005524;
GO  GO:0004197;
GO  GO:0003723;
GO  GO:0003724;
GO  GO:0003968;
GO  GO:0006351;
GO  GO:0006508;
GO  GO:0018144;
GO  GO:0039694;
TP  Membrane Topology: Transmembrane; Source: UniProt - Sequence Analysis {ECO:0000255};
SQ  MDSETIDMCVKFLKISFGLQSLKNLVKELFGGSELEKLAYVHAAFFHANEMAIHWNADLPWEEVMSSKRIKERFGYVKSH
SQ  FLRNVVYNADASGQMIRYNTTTCEQWFCCNFNLASYSASYNSLVPEEGGSMPINEEAEIKIGQSLLTCAQSVTKAIYAKL
SQ  STLTTRSIQGFLECLRDAICGAFSSWLPCIRGAFAWFGNIIEVLKHWAGAAHEKLHNFLEGIEECLYMGLGLVASTCIVA
SQ  LIEKFLVTMSVISGPCGRPTLFLTSAMAIISSTYLLSKAVEKSSAFTMLLGFVTQSCQTVLGSLFGKSAKGSEEAQGQFG
SQ  PSAMLESLATLVSSWSSSSVTEIGRTFGAISQIKNGIIALKDMALFVFSKLCEMASKVLGFESQILADLSIILGENVADW
SQ  LDECDCMLAYLLEFNSNARDIFDRLSQLIEKGKAIRMGILRTTHRGPSQVLSLVTKALDKLTELHNSVIMSGANSTRKTP
SQ  FMLFFTGKSGVGKTSVVQRMAANWLQQEQLGSNEVYSRNGLDPFWSGYKRQAVVTYDDFGAVPGSVSNEAEIINVVSSNP
SQ  HSVMMADLKEKGMYFDSRLIIASSNFLAANPESGVHDSEAYERRRHVVVQVSLKEDMAYDPGNPCANQRYTLLESKAPFA
SQ  EKAVFESYEELWSHVYNAFKAHEEKEKLFLSSLPIPERSEKEALQALIGICVMTTSYAPKAVIQYGIDHLVGYHYLISSA
SQ  EHVYFWHEKGEVEIVPMHLMKLDKMDKATMASTSLKSALMCQDMAKNFPTLNPLAVLYAKNIVIRGWVDANLQASKKCED
SQ  SYMREQIESLPKWQRAYLHVLSGHIASNETRGWFLNCLEVTKSNSRSSYIWEYKSWPMPLKLALGSFLAILAGSAIFCSL
SQ  QSLWSISGNASFVAGAASIFTIGSATAQSAPPNKDGSEYTYRNKKIKIRNWEGQGPCFGDSALWIAENCMATLVVMKDRV
SQ  QVCMAPGRSFLGVNHFLRMIPNGVMVKLETGMTETYFVWEKSKLKLFENSEIALYTSSNLPKAPDSLVDRFHFDLETLPK
SQ  TFPAQFFTYKFDKDMQQYVPELGELLCKKAERALCVVSGEYRRVISHHLTYRNPTVAGDCGGLVLAIIEGKCKLVGLHVA
SQ  SDGEEGAASPVPWDPDFKVAQGQSDFLLSYDEWAVPKVLGPGCKAVGIISPEHTVGSGGKTSFLETPIEWQLNRPCGKIP
SQ  SILVKGDVRLAGTENADYDPFAVGMTKYAKEAGPFEPNGLDRVCESIAETWHDASDGFEFGPVDLEAALNGIENMEYFDA
SQ  LVLSTSEGYPYRLDRKPGEKGKARYVEGEPGNLEITDERILADIHWFEEISKTQVPDLYCIECVKDERLPVRKVIKEPKS
SQ  RLFTVLPMSYNLAIRKKFLNFVRFIMKRRDVLPCQVGINPYSRQWGKVADRLLEKGNSILCCDYSRFDGFLPKCIMVKIA
SQ  EMFSNIVGETGAEREQTKNLMLACCSRYAICGRVLYRVENGIPSGFPLTVIVNSILNEILIKYAYWKCFETESLIRDHFD
SQ  TYVAMVVYGDDNLISVSEAISSKFNGNFLVNFMCNLGIKVTDGVDKTKVGIEFRTIEDCDFLKRKFKENADGTWSGVMAE
SQ  EHLWPQLHFVKAKKVEMSEAYISNCNNILRELWLGSPEKAAAFRREVISKLKWVEPQRLLTISQVALFHNEQMNGEHPFV
SQ  EACHQLENLELMAPLEPGMLPIKTQEIMPGLFVASEKNFTGNFDDYFTISITTNRKFEDGKGFQIIFPYGAGRGGLPSKA
SQ  FMEQNVIRKGCAIQKAFKQGLEKGNKMLFISQSSVIPAYVFAIMLYRSVDRLPRALSNKALTSALGICKKLSYLPKDFPD
SQ  LF
//