ID  Q80YF0;
PN  Mitotic spindle assembly checkpoint protein MAD1;
GN  MAD1L1;
OS  10029;
SL  Nucleus Position: SL-0178;
SL  Comments: Nucleus {ECO:0000250|UniProtKB:Q9Y6D9}. Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q9Y6D9}. Nucleus envelope {ECO:0000250|UniProtKB:Q9Y6D9}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000250|UniProtKB:Q9Y6D9}. Note=Detected at the nucleus envelope during interphase. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Detected at kinetochores during prometaphase. Colocalizes with NEK2 at the kinetochore. Colocalizes with IK at spindle poles during metaphase and anaphase. {ECO:0000250|UniProtKB:Q9Y6D9}.
DR  UNIPROT: Q80YF0;
DR  Pfam: PF05557;
DE  Function: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (By similarity). Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling (By similarity). {ECO:0000250|UniProtKB:Q9Y6D9}.
DE  Reference Proteome: No;
GO  GO:0000776;
GO  GO:1990706;
GO  GO:0005815;
GO  GO:0072686;
GO  GO:1990728;
GO  GO:0097431;
GO  GO:0044615;
GO  GO:0005634;
GO  GO:0042802;
GO  GO:0043515;
GO  GO:0051301;
GO  GO:0007094;
GO  GO:0042130;
GO  GO:0090267;
GO  GO:0090235;
GO  GO:0048538;
TP  Membrane Topology: Unknown; Source: UniProt - Sequence Analysis;
SQ  MEDLGENTTVLSTLRSLNNFISQRVEGGSGLDVSTAAPGSLQLQYEQSMQLEERAEQIRSKSYLIQMEREKMQMELSHKR
SQ  ARVELERAANTSARNYEREVDRNQELLARIRQLQEREAAAEEKMQEQLERHRLCKQSLDAASQQLREREDGLAAARETIS
SQ  SLKGRVSEMQLNAMDQKVQVKRLESEKQELKEQLELQQRKCQEASQKIQELQASQEERADHEQKIKDLEQKLCLQEQDAA
SQ  VVKNMKSELLRLPRMERELKRLREENTHLREMKETNGLLTEELEGLQRKLGRQEKMQEALVDLELEKEKLLAKLQSWEKL
SQ  DQTMGVNLRTPEDLSRFVVELQQRELTLKEKNNTITSSARGLEKAQQQLQDEVRQVSAQLLEERKKREIHEALARRLQKR
SQ  IVLLTKERDGMRAILGSYDSELTQAEYSAQLTQRMWEAEDMVQKVHAHSSEMETQLSQALEELGVQKQRADTLEMELKML
SQ  RAQTSSAETSFPFCKEEVDALRLKVEELEGERSRLEQEKQALEMQMERLTLQGDYNQSRTKVLHMSLNPASMARKRQQED
SQ  HARLQGECERLRGLVHALERGGPIPADLEVASSLPSSKEVAELRKQVESAELKNQRLKEVFQTKIQEFRKVCYTLTGYQI
SQ  DVTTENQYRLTSRYAEHQSDCLIFKATGPSGSKMQLLETEFSRSVPELIELHLLQQDSIPAFLSALTIELFSRQTSI
//