ID  Q8TB72;
PN  Pumilio homolog 2;
GN  PUM2;
OS  9606;
SL  Nucleus Position: SL-0198;
SL  Comments: Cytoplasm {ECO:0000305|PubMed:19168546}. Cytoplasmic granule {ECO:0000269|PubMed:25340845}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:19168546}. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules. Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells. {ECO:0000269|PubMed:25340845}.
DR  UNIPROT: Q8TB72;
DR  UNIPROT: B3KSL0;
DR  UNIPROT: B4E2B6;
DR  UNIPROT: D6W527;
DR  UNIPROT: O00234;
DR  UNIPROT: Q53TV7;
DR  UNIPROT: Q8WY43;
DR  UNIPROT: Q9HAN2;
DR  PDB: 3Q0Q;
DR  PDB: 3Q0R;
DR  PDB: 3Q0S;
DR  Pfam: PF00806;
DR  PROSITE: PS50302;
DR  PROSITE: PS50303;
DR  OMIM: 607205;
DR  DisGeNET: 23369;
DE  Function: Sequence-specific RNA-binding protein that acts as a post- transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'- UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation- independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). May regulate DCUN1D3 mRNA levels (PubMed:25349211). May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (PubMed:28431233). {ECO:0000269|PubMed:18776931, ECO:0000269|PubMed:21397187, ECO:0000269|PubMed:22345517, ECO:0000269|PubMed:22955276, ECO:0000269|PubMed:25340845, ECO:0000269|PubMed:25349211, ECO:0000269|PubMed:26724866, ECO:0000269|PubMed:28431233}.
DE  Reference Proteome: Yes;
DE  Interaction: Q9ULK4; IntAct: EBI-311679; Score: 0.37
DE  Interaction: Q9NQZ3; IntAct: EBI-997951; Score: 0.51
DE  Interaction: Q8ZJF3; IntAct: EBI-2860212; Score: 0.00
DE  Interaction: Q13573; IntAct: EBI-7950968; Score: 0.35
DE  Interaction: Q9H1Y0; IntAct: EBI-3622507; Score: 0.35
DE  Interaction: Q9Q2G4; IntAct: EBI-6174875; Score: 0.56
DE  Interaction: Q6SPF0; IntAct: EBI-11160311; Score: 0.35
DE  Interaction: Q8IUH3; IntAct: EBI-11161115; Score: 0.35
DE  Interaction: P47902; IntAct: EBI-21581572; Score: 0.35
DE  Interaction: O43251; IntAct: EBI-21595758; Score: 0.35
DE  Interaction: Q99732; IntAct: EBI-21643847; Score: 0.35
DE  Interaction: Q12905; IntAct: EBI-21665924; Score: 0.35
DE  Interaction: Q12926; IntAct: EBI-21666158; Score: 0.35
DE  Interaction: Q8N2M8; IntAct: EBI-21714908; Score: 0.35
DE  Interaction: Q8N6W0; IntAct: EBI-21727590; Score: 0.35
DE  Interaction: Q96SI9; IntAct: EBI-21752787; Score: 0.35
DE  Interaction: Q9UPY3; IntAct: EBI-20621391; Score: 0.35
DE  Interaction: P03372; IntAct: EBI-21301141; Score: 0.35
DE  Interaction: P35226; IntAct: EBI-27109494; Score: 0.35
DE  Interaction: Q92904; IntAct: EBI-27095486; Score: 0.37
DE  Interaction: Q8N9W6; IntAct: EBI-27095489; Score: 0.51
DE  Interaction: Q8TB72; IntAct: EBI-27095542; Score: 0.40
DE  Interaction: Q9BZB8; IntAct: EBI-29014562; Score: 0.27
GO  GO:0005737;
GO  GO:0010494;
GO  GO:0005829;
GO  GO:0031965;
GO  GO:0048471;
GO  GO:0106222;
GO  GO:0035198;
GO  GO:0003730;
GO  GO:0003729;
GO  GO:0062104;
GO  GO:0003723;
GO  GO:0060612;
GO  GO:0051276;
GO  GO:0001942;
GO  GO:0035196;
GO  GO:0007005;
GO  GO:2000637;
GO  GO:1900246;
GO  GO:0010608;
GO  GO:0051983;
GO  GO:0060964;
GO  GO:0043488;
GO  GO:0006417;
GO  GO:0022904;
GO  GO:0001501;
GO  GO:0034063;
TP  Membrane Topology: Unknown; Source: UniProt - Sequence Analysis;
SQ  MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGASHHSMSQPIMVQRRSGQGFHGNSEVNAI
SQ  LSPRSESGGLGVSMVEYVLSSSPADKLDSRFRKGNFGTRDAETDGPEKGDQKGKASPFEEDQNRDLKQGDDDDSKINGRG
SQ  LPNGMDADCKDFNRTPGSRQASPTEVVERLGPNTNPSEGLGPLPNPTANKPLVEEFSNPETQNLDAMEQVGLESLQFDYP
SQ  GNQVPMDSSGATVGLFDYNSQQQLFQRTNALTVQQLTAAQQQQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAAPPG
SQ  TDPYTAAGLAAAATLAGPAVVPPQYYGVPWGVYPANLFQQQAAAAANNTASQQAASQAQPGQQQVLRAGAGQRPLTPNQG
SQ  QQGQQAESLAAAAAANPTLAFGQGLATGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPTPVLISSAAAQAAA
SQ  AAAAGGTASSLTGSTNGLFRPIGTQPPQQQQQQPSTNLQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSTSLGFGSGNSLG
SQ  AAIGSALSGFGSSVGSSASSSATRRESLSTSSDLYKRSSSSLAPIGQPFYNSLGFSSSPSPIGMPLPSQTPGHSLTPPPS
SQ  LSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSASSTSSLFSSSSQLFPPSRLRYNRSDIMPSGRSRLLEDFRNNRFP
SQ  NLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQMVFNEILQAAYQLMTDVFGNYVIQKFFEFGSLDQKLALATRIR
SQ  GHVLPLALQMYGCRVIQKALESISSDQQVISEMVKELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVL
SQ  STHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASN
SQ  VVEKCVTHASRAERALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHI
SQ  LAKLEKYYLKNSPDLGPIGGPPNGML
//