ID  Q99222;
PN  ARF3-interacting protein 1;
GN  AFI1;
OS  559292;
SL  Nucleus Position: SL-0198;
SL  Comments: Cytoplasm, perinuclear region. Cytoplasm, cell cortex. Note=Enriched at the nuclear envelope and at the plasma membrane, especially in daughter cells and at the bud neck.
DR  UNIPROT: Q99222;
DR  UNIPROT: D6W2I7;
DR  UNIPROT: Q92274;
DR  Pfam: PF07792;
DR  PROSITE: PS50211;
DE  Function: Involved in actin patch polarization. Required for maintaining a proper budding pattern in yeast cells. Required for proper polarized localization of the ADP-ribosylation factor ARF3 at the plasma membrane. {ECO:0000269|PubMed:18397879}.
DE  Reference Proteome: Yes;
DE  Interaction: P53865; IntAct: EBI-1781812; Score: 0.55
DE  Interaction: P29366; IntAct: EBI-7365092; Score: 0.37
DE  Interaction: P11484; IntAct: EBI-3700856; Score: 0.35
DE  Interaction: P32589; IntAct: EBI-3732939; Score: 0.35
GO  GO:0005938;
GO  GO:0005935;
GO  GO:0005737;
GO  GO:0005634;
GO  GO:0048471;
GO  GO:0005886;
GO  GO:0051666;
GO  GO:0000282;
TP  Membrane Topology: Unknown; Source: UniProt - Sequence Analysis;
SQ  MLRRELNNSISNRSIENESFPFERPNVSYIISAEFDNKLGPILKHQYPKDIPGFNQFSHEQRNGNTSVSMNLASLMIPSS
SQ  IERNPGKQDITVFTLYYNKFTQNYQLFPVPKDPRFSFNLHHREQSDGSVTNSIYYDAENHQDAKNNRYTIVLEDDELECQ
SQ  EVQNNQKAIDNEPLFFINVANTVLDTTNDRGAVIKSIAIGTPLKTFFAFKNIIVLVLDLYMKAPTQAAATDILLDCFNML
SQ  NSIDLTLINDIHSKSSIQEVLHSIHDESIITKVFLDPDSTLKKLFCINGFDTKDKYGNIVTFHDQLIQYHFTRFQPKTLP
SQ  PFLLKIPLQFNMIRREPIYIENDYNELVLKFLDKFVPYLLKAGQKVNAWKLVINSTKLSKEDLCAFILSLANITATYASD
SQ  PQSYFKGNAALIFPYMDISLVDGLRAYVASNSDFVGCFAIIGTANPIFRYQLDIWDYYYDVDEGVFYENNSPEKEKPDTV
SQ  AEVKIGPNPLRKIFNRPHFSTNAVNESQVNLGQKLFSLLIDEYHDSDTIMSVLRRLNVLQLENLLDALKRREIPPNIALK
SQ  DEYIMFYKDFFIFPEFFDYFTLHSIELLSNLDNCLFSLGNTCQLFSTEQIYSQLSQILDIVKELFRMVSVSRTNIEKFLN
SQ  ACLNYSPFKILPTAQLHGDNISRWSFESEVRQGFDNFNSYMGIEKDPHGVIVSAIDLFTQIYSFDILAFFLTFITKESGQ
SQ  DLPFTKSLSRRRTYLTRIAQSSSLRQFLQLSTRPNIRILGGNGQGTGNSNYPEFTNASSVISPKLRASPLLERRASKICY
SQ  AITKLLYRLECHPIGMALLKKYLHNQLREAYLESKRHFISKKGDSTNTSSTIASSSFAGASVPLSSNESGMLNGLKQINE
SQ  QQESTLETTQKED
//