ID  Q9JJF2;
PN  Sperm-associated antigen 4 protein;
GN  Spag4;
OS  10090;
SL  Nucleus Position: SL-0178;
SL  Nucleus Position: SL-0179;
SL  Nucleus Position: SL-0182;
SL  Comments: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Cytoplasm, cytoskeleton {ECO:0000250}. [Isoform 1]: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:O55034}. Nucleus envelope {ECO:0000269|PubMed:26621829}. Nucleus inner membrane {ECO:0000305}. Cytoplasm, cytoskeleton, flagellum axoneme {ECO:0000250|UniProtKB:O55034}. Note=In spermatids, isoform 1 is localized in the transient manchette and in the axoneme of elongating spermatids and epididymal sperm (By similarity). Conflictingly is not found in axoneme but only associated with the manchette where cytoplasmic microtubules contact the nuclear envelope. Localizes at the posterior lateral side of round and elongating spermatids (PubMed:26621829). {ECO:0000250|UniProtKB:O55034, ECO:0000269|PubMed:26621829}.
DR  UNIPROT: Q9JJF2;
DR  UNIPROT: A3KGK4;
DR  Pfam: PF07738;
DR  PROSITE: PS51469;
DE  Function: Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. {ECO:0000269|PubMed:26621829}.
DE  Reference Proteome: Yes;
DE  Interaction: Q5SS91; IntAct: EBI-12557506; Score: 0.51
DE  Interaction: Q6ZWR6; IntAct: EBI-12557523; Score: 0.46
DE  Interaction: Q9JJF2; IntAct: EBI-12557600; Score: 0.46
GO  GO:0005737;
GO  GO:0005639;
GO  GO:0034993;
GO  GO:0005874;
GO  GO:0031514;
GO  GO:0005635;
GO  GO:0042802;
GO  GO:0043495;
GO  GO:0030154;
GO  GO:0006998;
GO  GO:0007283;
TP  Membrane Topology: Transmembrane; Source: UniProt - Sequence Analysis {ECO:0000255};
SQ  MRRSPRSGSAASSHNHTPNFYSENSNSSHSATSGDSNGRRSAGPELGEPEGRRARGSSCGEPALSPGMPGGDTWAGSSRP
SQ  KLAPRSHNGQTACGAATVRGGASEPSGSSVVLEEQLNLLPILDLRQEMPTPRVSKSFLSLLFQVLSMVLSLAVDGLVCVC
SQ  REICSIRFLFTAVSLLSIFLAALWWGLLYLIPPLENEPTEMLTLSQYHHRVHSQGQQLQQLQAELNKLHKEVSSVRAAHS
SQ  ERVAKLVFQRLNEDFVRKPDYALSSVGASIDLEKTSSDYEDQNTAYFWNRLSFWNYARPPSVILEPDVFPGNCWAFEGDK
SQ  GQVVIRLPGHVQLSDITLQHPPPTVAHTGGASSAPRDFAVYGLQADDETEVFLGKFIFDVQKSEIQTFHLQNDPPSAFPK
SQ  VKIQILSNWGHPRFTCLYRVRAHGVRTSEWADDNATGVTGGPH
//