ID  Q9LTY1;
PN  Mitotic spindle checkpoint protein MAD1;
GN  MAD1;
OS  3702;
SL  Nucleus Position: SL-0178;
SL  Comments: Nucleus envelope {ECO:0000269|PubMed:22457071}. Note=The nucleus envelope association requires the presence of NUA. {ECO:0000269|PubMed:22457071}.
DR  UNIPROT: Q9LTY1;
DR  Pfam: PF05557;
DE  Function: Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Required for anchoring MAD2 to the nuclear envelope. {ECO:0000269|PubMed:22457071}.
DE  Reference Proteome: Yes;
GO  GO:0000776;
GO  GO:0072686;
GO  GO:0005635;
GO  GO:0051315;
GO  GO:0051301;
GO  GO:0007094;
TP  Membrane Topology: Unknown; Source: UniProt - Sequence Analysis;
SQ  MILRTPQPKRLRSDAGESPFPTGATGSGNQLIIYEDSPLPAPAPLQTSHDHSADQHLCTYQCRQMVKADVLDALSTAEKQ
SQ  VEESKTKLQTLNANFTEADAERKHFRDKFLYSEQELAAAKAREKMLQEQLLMEINNSQERYTKELQSCHELEVKLQNEMN
SQ  LRKKAESSAATAEEKAKLLEDKLTQLSGSVDREKKRLNNDIAQLGKEAKLSVARIGADLERMQCRAQNAETESNLLRSQL
SQ  EHLKLIFDECLQEKTEVDKKLSSFTSEAASSSDNSVLVKHLQEELKRYEAEVREARKLKSRHLDAELLNVNLLEEQSRRE
SQ  RAESELSKFHDLQLSMEKLENELSSWKSLLNDIPGVSCPDDIVMRFSVLQNEVVQSTMKIGEASTRIKQLEETLEAIQLG
SQ  RQNAVSEAALAKEKSEALKTDVKRIEVMLTLVTEEKEQLKAVVNELRKSNSEGSVSGAADGALIQGFESSLAKKENYIKD
SQ  LEQDLNQLKDVNNRQRTEIELLNEKLVDEARRNKSLERDSDRLRSEISLLESKLGHGDYSAANTRVLRMVNTLGVENEAK
SQ  QTIEALQAELQKTKERLQAVEELKSQSGDAGKLVDSHITGKIAQLKEQNATLEKREERYKTVFADRISVFRRACCELFGY
SQ  KIVMDEHQRPNGIPVTRFTLQSIYAQSDDEKLEFEYESGNTSILNNEYASQGDIAKQIEIFIRKFNSIPAFTANLTMESF
SQ  NRRTLY
//