ID  Q9N3Z3;
PN  Serine/threonine-protein kinase chk-1;
GN  chk;
OS  6239;
SL  Nucleus Position: SL-0198;
SL  Comments: Cytoplasm {ECO:0000269|PubMed:26073019}. Nucleus {ECO:0000250|UniProtKB:O61661}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:26073019}. Note=The Ser-344 phosphorylated form colocalizes with P granules in a perinuclear manner in embryonic germline precursor cells and in Z2/Z3 primordial germ cells in L1 stage larvae. {ECO:0000269|PubMed:26073019}.
DR  UNIPROT: Q9N3Z3;
DR  UNIPROT: Q17375;
DR  UNIPROT: Q86FM7;
DR  Pfam: PF00069;
DR  PROSITE: PS00107;
DR  PROSITE: PS50011;
DR  PROSITE: PS00108;
DE  Function: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA (PubMed:15326393). May also negatively regulate cell cycle progression during unperturbed cell cycles (PubMed:15326393). Required for checkpoint mediated cell cycle arrest in response to DNA damage in germline cells (PubMed:15326393, PubMed:27956467). Delays cell-cycle reentry of the Z2 and Z3 primordial germ cells in response to transcription-induced DNA damage as they emerge from cell cycle arrest in L1 larvae (PubMed:26073019). Essential for embryogenesis (PubMed:15326393). {ECO:0000269|PubMed:15326393, ECO:0000269|PubMed:26073019, ECO:0000269|PubMed:27956467}.
DE  Reference Proteome: Yes;
DE  Interaction: Q9XTY9; IntAct: EBI-344141; Score: 0.37
DE  Interaction: Q9N4A9; IntAct: EBI-344144; Score: 0.37
GO  GO:0005634;
GO  GO:0048471;
GO  GO:0005524;
GO  GO:0106310;
GO  GO:0004674;
GO  GO:0000077;
GO  GO:0009792;
GO  GO:0035556;
GO  GO:0033314;
GO  GO:0006468;
TP  Membrane Topology: Unknown; Source: UniProt - Sequence Analysis;
SQ  MSAASTTSTPAAAAVAPQQPESLYRVVQTLGEGAFGEVLLIVNTKNPEVAAAMKKINIANKSKDFIDNIRKEYLLQKRVS
SQ  AVGHDNVIRMIGMRNDPQFYYLFLEYADGGELFDKIEPDCGMSPVFAQFYFKQLICGLKFIHDNDVVHRDIKPENLLLTG
SQ  THVLKISDFGMATLYRNKGEERLLDLSCGTIPYAAPELCAGKKYRGPPVDVWSSGIVLIAMLTGELPWDRASDASQSYMG
SQ  WISNTSLDERPWKKIDVRALCMLRKIVTDKTDKRATIEQIQADPWYQHNFGQVETPNGRPLKRARNNDENITCTQQAECS
SQ  AKRRHLETPNEKSTLAERQNASFSQPTKTEDLLLTQHIDMSQTNSNLLQRMVCRMTRFCVVTDIRSTYQKVARASEHAGF
SQ  GVRETDDYRLLVTWREVSMMVSLYTMGDIPDKPRVMVDFRRSRGDGIQFKKMFMDVRNRMHEWICTDGNNWLANLGYVPR
SQ  NPQIVNGGGVNVEHSASSINVDV
//