ID  Q9Y4G8;
PN  Rap guanine nucleotide exchange factor 2;
GN  RAPGEF2;
OS  9606;
SL  Nucleus Position: SL-0198;
SL  Comments: Cytoplasm. Cytoplasm, perinuclear region. Cell membrane. Late endosome. Cell junction {ECO:0000250}. Note=Associated with the synaptic plasma membrane. Colocalizes with ADRB1 at the plasma membrane. Synaptosome. Enriched in synaptic plasma membrane and neuronal cell body. Colocalized with CTNNB1 at cell-cell contacts (By similarity). Localized diffusely in the cytoplasm before neuronal growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with the high affinity nerve growth factor receptor NTRK1 at late endosomes. Translocated to the perinuclear region in a RAP1A-dependent manner. Translocated to the cell membrane. {ECO:0000250}.
DR  UNIPROT: Q9Y4G8;
DR  UNIPROT: D3DP27;
DR  PDB: 6QDT;
DR  Pfam: PF00595;
DR  Pfam: PF00788;
DR  Pfam: PF00617;
DR  Pfam: PF00618;
DR  PROSITE: PS50042;
DR  PROSITE: PS50106;
DR  PROSITE: PS50200;
DR  PROSITE: PS50009;
DR  PROSITE: PS50212;
DR  OMIM: 609530;
DR  OMIM: 618075;
DR  DisGeNET: 9693;
DE  Function: Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP (PubMed:23800469, PubMed:10801446) or not (PubMed:10608844, PubMed:10548487, PubMed:11359771). Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP- independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions. {ECO:0000269|PubMed:10548487, ECO:0000269|PubMed:10608844, ECO:0000269|PubMed:10608883, ECO:0000269|PubMed:10801446, ECO:0000269|PubMed:10934204, ECO:0000269|PubMed:11359771, ECO:0000269|PubMed:12391161, ECO:0000269|PubMed:16272156, ECO:0000269|PubMed:17724123, ECO:0000269|PubMed:21840392, ECO:0000269|PubMed:23800469}.
DE  Disease: Epilepsy, familial adult myoclonic, 7 (FAME7) [MIM:618075]: A form of familial myoclonic epilepsy, a neurologic disorder characterized by cortical hand tremors, myoclonic jerks and occasional generalized or focal seizures with a non-progressive or very slowly progressive disease course. Usually, myoclonic tremor is the presenting symptom, characterized by tremulous finger movements and myoclonic jerks of the limbs increased by action and posture. In a minority of patients, seizures are the presenting symptom. Some patients exhibit mild cognitive impairment. FAME7 inheritance is autosomal dominant. {ECO:0000269|PubMed:29507423}. Note=The disease is caused by variants affecting the gene represented in this entry.
DE  Reference Proteome: Yes;
DE  Interaction: P01112; IntAct: EBI-8766313; Score: 0.44
DE  Interaction: P62834; IntAct: EBI-8766309; Score: 0.44
DE  Interaction: Q12840; IntAct: EBI-3450200; Score: 0.00
DE  Interaction: Q9Y3A3; IntAct: EBI-3450242; Score: 0.00
DE  Interaction: P41743; IntAct: EBI-2434129; Score: 0.35
DE  Interaction: Q86UL8; IntAct: EBI-7129086; Score: 0.52
DE  Interaction: Q99623; IntAct: EBI-1084965; Score: 0.00
DE  Interaction: P63104; IntAct: EBI-7195964; Score: 0.40
DE  Interaction: P03928; IntAct: EBI-3450122; Score: 0.00
DE  Interaction: P08107; IntAct: EBI-3450193; Score: 0.00
DE  Interaction: P33176; IntAct: EBI-3450207; Score: 0.00
DE  Interaction: P31016; IntAct: EBI-7958027; Score: 0.44
DE  Interaction: P46934; IntAct: EBI-7026879; Score: 0.44
DE  Interaction: Q62940; IntAct: EBI-7026897; Score: 0.44
DE  Interaction: Q96PU5; IntAct: EBI-7026931; Score: 0.44
DE  Interaction: B1AYL1; IntAct: EBI-8068510; Score: 0.44
DE  Interaction: P46937; IntAct: EBI-6912563; Score: 0.53
DE  Interaction: Q96QZ7; IntAct: EBI-8769602; Score: 0.52
DE  Interaction: Q9H0H5; IntAct: EBI-11003256; Score: 0.35
DE  Interaction: P26041; IntAct: EBI-11010611; Score: 0.35
DE  Interaction: Q9UJA5; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q14696; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q6BDS2; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q96L50; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q96KM6; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: P42858; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q8N9Q2; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: A6H8Y1; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: C9J1X0; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q9BYT9; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q99442; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q96IX5; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: O43414; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: P20674; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: P98170; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: G5E9A6; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q9H930; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q14558; IntAct: EBI-11041084; Score: 0.35
DE  Interaction: Q96JG6; IntAct: EBI-11134088; Score: 0.35
DE  Interaction: Q6P5F9; IntAct: EBI-11603310; Score: 0.35
DE  Interaction: Q16695; IntAct: EBI-20921180; Score: 0.40
DE  Interaction: P16401; IntAct: EBI-20925170; Score: 0.40
GO  GO:0016324;
GO  GO:0005923;
GO  GO:0005911;
GO  GO:0005737;
GO  GO:0005829;
GO  GO:0030139;
GO  GO:0005887;
GO  GO:0005770;
GO  GO:0016020;
GO  GO:0043005;
GO  GO:0043025;
GO  GO:0048471;
GO  GO:0005886;
GO  GO:0032991;
GO  GO:0045202;
GO  GO:0031697;
GO  GO:0005509;
GO  GO:0030552;
GO  GO:0019992;
GO  GO:0005096;
GO  GO:0005085;
GO  GO:0030165;
GO  GO:0070300;
GO  GO:0050699;
GO  GO:0071880;
GO  GO:0001568;
GO  GO:0031547;
GO  GO:0019933;
GO  GO:0071320;
GO  GO:0071321;
GO  GO:1990090;
GO  GO:0061028;
GO  GO:0090557;
GO  GO:0021884;
GO  GO:0007186;
GO  GO:0035556;
GO  GO:0000165;
GO  GO:0030033;
GO  GO:0008285;
GO  GO:0050774;
GO  GO:0048022;
GO  GO:0038180;
GO  GO:0001764;
GO  GO:0031175;
GO  GO:0007218;
GO  GO:2000481;
GO  GO:0043950;
GO  GO:2000670;
GO  GO:0070374;
GO  GO:0043547;
GO  GO:2001224;
GO  GO:0010976;
GO  GO:0032092;
GO  GO:0045860;
GO  GO:2001214;
GO  GO:0072659;
GO  GO:0032486;
GO  GO:0007265;
GO  GO:1901888;
GO  GO:0048167;
GO  GO:0007264;
GO  GO:0021591;
TP  Membrane Topology: Unknown; Source: UniProt - Sequence Analysis;
SQ  MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPE
SQ  TAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAG
SQ  TIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEK
SQ  NMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWF
SQ  NDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFI
SQ  LLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEE
SQ  KRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQD
SQ  DSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIRE
SQ  FAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVE
SQ  VATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRE
SQ  CKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFL
SQ  HEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSS
SQ  FLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQP
SQ  QQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTS
SQ  PQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQY
SQ  SLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFD
SQ  YSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSS
SQ  LTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVAR
SQ  SSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV
//