Membrane {Sequence AnalysisHAMAP-Rule:MF_03021}; Peripheral membrane protein {Sequence AnalysisHAMAP-Rule:MF_03021}. Endoplasmic reticulum {Sequence AnalysisHAMAP-Rule:MF_03021}. Midbody {Sequence AnalysisHAMAP-Rule:MF_03021}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {Sequence AnalysisHAMAP-Rule:MF_03021}. Cytoplasm, cytoskeleton {Sequence AnalysisHAMAP-Rule:MF_03021}. Cytoplasm, perinuclear region {Sequence AnalysisHAMAP-Rule:MF_03021}. Nucleus {Sequence AnalysisHAMAP-Rule:MF_03021}. Cytoplasm, cytoskeleton, spindle {Sequence AnalysisHAMAP-Rule:MF_03021}. Cytoplasm {ECO:0000255|HAMAP- Rule:MF_03021}. Cell projection, axon {ECO:0000250|UniProtKB:Q9UBP0}. Note=Forms an intramembrane hairpin-like structure in the membrane. Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B (By similarity). Enriched in the distal axons and branches of postmitotic neurons (By similarity). Evenly distributed along early axons and concentrates in the growth cone of the axons of late stage 3 neurons (PubMed:18234839). Mainly nuclear in interphase cells and becomes associated with the centrosomes, spindle microtubules, midzone and finally the midbody during cell division (By similarity). {ECO:0000250|UniProtKB:Q9UBP0, Sequence AnalysisHAMAP-Rule:MF_03021, ECO:0000269|PubMed:18234839}. [Isoform 1]: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9UBP0}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9UBP0}. Nucleus membrane {ECO:0000250|UniProtKB:Q9UBP0}. Lipid droplet {ECO:0000250|UniProtKB:Q9UBP0}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q9UBP0}. Endosome {ECO:0000250|UniProtKB:Q9UBP0}. Note=Forms an intramembrane hairpin- like structure in the membrane. Recruited to nuclear membrane by IST1 during late anaphase. Localizes to endoplasmic reticulum tubular network. {ECO:0000250|UniProtKB:Q9UBP0}. [Isoform 2]: Cytoplasm {ECO:0000250|UniProtKB:Q9UBP0}. Endosome {ECO:0000250|UniProtKB:Q9UBP0}. Nucleus membrane {ECO:0000250|UniProtKB:Q9UBP0}. Note=Constitutes the main endosomal form. Recruited to nuclear membrane by IST1 during late anaphase. {ECO:0000250|UniProtKB:Q9UBP0}.
The nuclear envelope is a membrane system which surrounds the nucleoplasm of eukaryotic cells. It is composed of the nuclear lamina, nuclear pore complexes and two nuclear membranes. The space between the two membranes is called the nuclear intermembrane space.
The membrane surrounding the nucleus. This term is used when it is not known if the protein is found in or associated with the inner or outer nuclear membrane.
ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:19141076 PubMed:20530212). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (By similarity). Severing activity is not dependent on tubulin acetylation or detyrosination (By similarity). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation (By similarity). It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia (By similarity). SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (By similarity). Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (By similarity). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (By similarity). Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (By similarity). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (By similarity). Probably plays a role in axon growth and the formation of axonal branches (PubMed:18234839). {By
SimilarityUniProtKB:Q9UBP0, Sequence AnalysisHAMAP-Rule:MF_03021, Experimental EvidencePubMed:18234839, Experimental EvidencePubMed:19141076, Experimental EvidencePubMed:20530212}. [Isoform 1]: Involved in lipid metabolism by regulating the size and distribution of lipid droplets. {By
SimilarityUniProtKB:Q9UBP0}.
Charged multivesicular body protein 3 (Chromatin-modifying protein 3) (Neuroendocrine differentiation factor) (Vacuolar protein sorting-associated protein 24) (hVps24)
Charged multivesicular body protein 2b (CHMP2.5) (Chromatin-modifying protein 2b) (CHMP2b) (Vacuolar protein sorting-associated protein 2-2) (Vps2-2) (hVps2-2)
Charged multivesicular body protein 1a (Chromatin-modifying protein 1a) (CHMP1a) (Vacuolar protein sorting-associated protein 46-1) (Vps46-1) (hVps46-1)
Charged multivesicular body protein 5 (Chromatin-modifying protein 5) (SNF7 domain-containing protein 2) (Vacuolar protein sorting-associated protein 60) (Vps60) (hVps60)
National and Kapodistrian University of Athens
Department of Biology
Biophysics & Bioinformatics Laboratory