The nuclear envelope is a membrane system which surrounds the nucleoplasm of eukaryotic cells. It is composed of the nuclear lamina, nuclear pore complexes and two nuclear membranes. The space between the two membranes is called the nuclear intermembrane space.
The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active bidirectional transport of macromolecules such as proteins, RNAs etc across the double-membrane nuclear envelope.The NPC is composed of at least 30 distinct subunits known as Nucleoporins (NUPs).
Maintains the proteasome and its anchor cut8 at the nucleus envelope and is required for kinetochore component proteostasis (PubMed:28974540). Proper kinetochore stoichiometry ensures the correct attachment of kinetochores to spindle microtubules during cytokinesis (PubMed:28974540, PubMed:10660053). Required for the localization of spindle assembly checkpoint (SAC) protein mad2 and bub1 to the nucleus envelope during interphase, but not their localization during mitosis (PubMed:28974540). {Experimental EvidencePubMed:10660053, Experimental EvidencePubMed:28974540}.
National and Kapodistrian University of Athens
Department of Biology
Biophysics & Bioinformatics Laboratory