Synaptosomal-associated protein 25 - Rattus norvegicus

Synaptosomal-associated protein 25 (Rattus norvegicus)

FASTA TEXT XML

Subcellular Location Biology & Function Interactions Cross-References

General Information

Protein Information
Protein Name	Synaptosomal-associated protein 25
Accession Code	P60881
Gene	Snap25
Organism	Rattus norvegicus \| Norway rat (Taxonomy: 10116)
Part of Reference Proteome?	Yes
Sequence (Length: 206)
MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTD LGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNL RHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG

Structure Viewer (PDB: 5LOB)

Subcellular Location

Description
Cytoplasm, perinuclear region {By SimilarityUniProtKB:P60879}. Cell membrane {Experimental EvidencePubMed:12403834, Experimental EvidencePubMed:23341457}; Lipid- anchor {By SimilarityUniProtKB:P60879}. Synapse, synaptosome {ECO:0000269\|PubMed:1281490}. Photoreceptor inner segment {By SimilarityUniProtKB:P60879}. Note=Membrane association requires palmitoylation (By similarity). Expressed throughout cytoplasm, concentrating at the perinuclear region (By similarity). Colocalizes with KCNB1 at the cell membrane (PubMed:12403834). Colocalizes with PLCL1 at the cell membrane (PubMed:23341457). {By SimilarityUniProtKB:P60879, Experimental EvidencePubMed:12403834, ECO:0000269\|PubMed:1281490, Experimental EvidencePubMed:23341457}.
Position in the Nuclear Envelope
Location	Location ID	Description
Perinuclear Region	SL-0198	The perinuclear region is the cytoplasmic region just around the nucleus.
Membrane Topology
Topology	Source	Annotation Type
Lipid-Anchored	UniProt	By Similarity {ECO:0000250\|UniProtKB:P60879}
Assigned Ontology terms

Cellular Component	Actin Cytoskeleton (GO:0015629) Obsolete Anchored Component Of Presynaptic Membrane (GO:0099026) Anchoring Junction (GO:0070161) Axon (GO:0030424) Axonal Growth Cone (GO:0044295) Cytoplasm (GO:0005737) Cytoskeleton (GO:0005856) Cytosol (GO:0005829) Endosome (GO:0005768) Extrinsic Component Of Cytoplasmic Side Of Plasma Membrane (GO:0031234) Filopodium (GO:0030175) Glutamatergic Synapse (GO:0098978) Growth Cone (GO:0030426) Intracellular Organelle (GO:0043229) Lamellipodium (GO:0030027) Membrane (GO:0016020) Membrane Raft (GO:0045121) Neuron Projection (GO:0043005) Neuronal Cell Body (GO:0043025) Perinuclear Region Of Cytoplasm (GO:0048471) Photoreceptor Inner Segment (GO:0001917) Plasma Membrane (GO:0005886) Postsynapse (GO:0098794) Presynaptic Active Zone Membrane (GO:0048787) Presynaptic Membrane (GO:0042734) Ribbon Synapse (GO:0097470) SNARE Complex (GO:0031201) Somatodendritic Compartment (GO:0036477) Synapse (GO:0045202) Synaptic Vesicle (GO:0008021) Synaptobrevin 2-SNAP-25-Syntaxin-1a Complex (GO:0070044) Synaptobrevin 2-SNAP-25-Syntaxin-1a-Complexin I Complex (GO:0070032) Synaptobrevin 2-SNAP-25-Syntaxin-1a-Complexin II Complex (GO:0070033) Terminal Bouton (GO:0043195) Trans-Golgi Network (GO:0005802) Voltage-Gated Potassium Channel Complex (GO:0008076)

Biology & Function

Description
t-SNARE involved in the molecular regulation of neurotransmitter release (PubMed:8243676, PubMed:8103915). May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells (PubMed:12403834). {Experimental EvidencePubMed:12403834, Curator InferencePubMed:8103915, Curator InferencePubMed:8243676}.
Assigned Ontology terms

Biological Process	Associative Learning (GO:0008306) Axonogenesis (GO:0007409) Calcium Ion-Regulated Exocytosis Of Neurotransmitter (GO:0048791) Endosomal Transport (GO:0016197) Exocytic Insertion Of Neurotransmitter Receptor To Postsynaptic Membrane (GO:0098967) Exocytosis (GO:0006887) Growth Hormone Secretion (GO:0030252) Locomotory Behavior (GO:0007626) Long-Term Memory (GO:0007616) Long-Term Synaptic Potentiation (GO:0060291) Neuron Differentiation (GO:0030182) Neurotransmitter Receptor Internalization (GO:0099590) Neurotransmitter Secretion (GO:0007269) Positive Regulation Of Hormone Secretion (GO:0046887) Positive Regulation Of Insulin Secretion (GO:0032024) Regulation Of Establishment Of Protein Localization (GO:0070201) Regulation Of Neuron Projection Development (GO:0010975) Regulation Of Synapse Assembly (GO:0051963) Sleep (GO:0030431) SNARE Complex Assembly (GO:0035493) Synaptic Vesicle Exocytosis (GO:0016079) Synaptic Vesicle Fusion To Presynaptic Active Zone Membrane (GO:0031629) Synaptic Vesicle Priming (GO:0016082) Vesicle Fusion (GO:0006906)
Molecular Function	Calcium-Dependent Protein Binding (GO:0048306) Myosin Binding (GO:0017022) Protein Domain Specific Binding (GO:0019904) Protein N-Terminus Binding (GO:0047485) SNAP Receptor Activity (GO:0005484) SNARE Binding (GO:0000149) Syntaxin Binding (GO:0019905) Syntaxin-1 Binding (GO:0017075) Transmembrane Transporter Binding (GO:0044325) Voltage-Gated Potassium Channel Activity (GO:0005249)

Protein-Protein Interactions

Interactions with Nuclear Envelope proteins (3 interactors)

Partner (NucEnvDB)		IntAct	Confidence score
P60881	Self	EBI-1027245	0.81
Q9WVE9	Intersectin-1	EBI-7031678	0.60
O95295	SNARE-associated protein Snapin	EBI-8753069	0.40
Interactions with other proteins (28 interactors)

Partner (UniProt)		IntAct	Confidence score
Q9JJ50	Hepatocyte growth factor-regulated tyrosine kinase substrate (SNAP-25-interacting protein Hrs-2)	EBI-7287330	0.53
P32851	Syntaxin-1A (Neuron-specific antigen HPC-1) (Synaptotagmin-associated 35 kDa protein) (P35A)	EBI-7031830	0.97
P21575	Dynamin-1 (EC 3.6.5.5) (B-dynamin) (D100) (Dynamin, brain)	EBI-7031910	0.40
P47709	Rabphilin-3A (Exophilin-1)	EBI-8545674	0.52
P63045	Vesicle-associated membrane protein 2 (VAMP-2) (Synaptobrevin-2)	EBI-1027245	0.95
Q9WU70	Syntaxin-binding protein 5 (Lethal(2) giant larvae protein homolog 3) (Tomosyn-1)	EBI-1039933	0.40
Q9QXY2	SRC kinase signaling inhibitor 1 (SNAP-25-interacting protein) (SNIP) (p130Cas-associated protein) (p140Cap)	EBI-1394117	0.54
Q63666	Vesicle-associated membrane protein 1 (VAMP-1) (Synaptobrevin-1)	EBI-7573643	0.46
Q63635	Syntaxin-6	EBI-7573728	0.40
G3V7P1	Syntaxin-12 (Syntaxin-13)	EBI-7573698	0.40
Q9WUF4	Vesicle-associated membrane protein 8 (VAMP-8) (Endobrevin) (EDB)	EBI-7573895	0.35
Q4KMA2	UV excision repair protein RAD23 homolog B	EBI-2640427	0.35
Q5S007	Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (EC 3.6.5.-) (Dardarin)	EBI-6665350	0.35
Q08850	Syntaxin-4	EBI-9822143	0.40
Q9BV40	Vesicle-associated membrane protein 8 (VAMP-8) (Endobrevin) (EDB)	EBI-9822143	0.40
Q15836	Vesicle-associated membrane protein 3 (VAMP-3) (Cellubrevin) (CEB) (Synaptobrevin-3)	EBI-9822137	0.40
P63027	Vesicle-associated membrane protein 2 (VAMP-2) (Synaptobrevin-2)	EBI-9822200	0.40
A0A142I9X8	11 kDa protein (Membrane protein US9) (Tegument protein US9)	EBI-11701393	0.35
Q08849	Syntaxin-3	EBI-15578272	0.61
O16000	Syntaxin-1A homolog (Uncoordinated protein 64)	EBI-15595291	0.40
P63041	Complexin-1 (Complexin I) (CPX I) (Synaphin-2)	EBI-15657265	0.35
P84087	Complexin-2 (Complexin II) (CPX II) (Synaphin-1)	EBI-15743043	0.35
P21707	Synaptotagmin-1 (Synaptotagmin I) (SytI) (p65)	EBI-15837855	0.44
Q9JHW5	Vesicle-associated membrane protein 7 (VAMP-7) (Synaptobrevin-like protein 1)	EBI-16138941	0.50
P18708	Vesicle-fusing ATPase (EC 3.6.4.6) (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein) (Vesicular-fusion protein NSF)	EBI-16139061	0.49
P54921	Alpha-soluble NSF attachment protein (SNAP-alpha) (N-ethylmaleimide-sensitive factor attachment protein alpha)	EBI-16139110	0.40
P19332	Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)	EBI-26374040	0.35
Q5XIE8	Integral membrane protein 2B (Immature BRI2) (imBRI2) (Transmembrane protein BRI) (Bri) [Cleaved into: BRI2, membrane form (Mature BRI2) (mBRI2); BRI2 intracellular domain (BRI2 ICD); BRI2C, soluble form; Bri23 peptide (Bri2-23) (ABri23) (C-terminal peptide) (P23 peptide)]	EBI-26438079	0.35

Cross-References

Database	Links
UNIPROT	P60881 P13795 P36974 P70557 P70558 Q8IXK3 Q96FM2 Q9BR45
PDB	1JTH 1N7S 1SFC 1URQ 3HD7 3IPD 3J96 3J97 3J98 3J99 5CCG 5CCH 5CCI 5KJ7 5KJ8 5LOB 5LOW 5W5C 5W5D 6IP1 6MDM 6MDN 6MDO 6MDP 6MTI 6WVW
Pfam	PF00835
PROSITE	PS50192

Synaptosomal-associated protein 25 (Rattus norvegicus)

General Information

Protein Information

Structure Viewer (PDB: 5LOB)

Subcellular Location

Description Show

Position in the Nuclear Envelope

Membrane Topology

Assigned Ontology terms

Biology & Function

Description Hide

Assigned Ontology terms

Protein-Protein Interactions

Interactions with Nuclear Envelope proteins (3 interactors)

Interactions with other proteins (28 interactors)

Cross-References

Database

Links

Description

Description