Nuclear mRNA export protein THP1 - Saccharomyces cerevisiae S288C

Nuclear mRNA export protein THP1 (Saccharomyces cerevisiae S288C)

FASTA TEXT XML

Subcellular Location Biology & Function Interactions Cross-References

General Information

Protein Information
Protein Name	Nuclear mRNA export protein THP1
Accession Code	Q08231
Gene	THP1
Organism	Saccharomyces cerevisiae S288C (Taxonomy: 559292)
Part of Reference Proteome?	Yes
Sequence (Length: 455)
MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTRFKIMCTSFLNYARDVDPWSA WSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFIINLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPR GNASSTNIPGKQRILLYLVNKLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAF VQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWSVLYKHVRYGNIQGVSLWLRQ NERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQNKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNV ENVLVTLINLGLLRANCFPQLQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW

Structure Viewer (PDB: 5UBP)

Subcellular Location

Description
Nucleus envelope {Experimental EvidencePubMed:12206772, Experimental EvidencePubMed:12411502, Experimental EvidencePubMed:14562095}. Note=Localizes to the nuclear pores.
Position in the Nuclear Envelope
Location	Location ID	Description
Nuclear Envelope	SL-0178	The nuclear envelope is a membrane system which surrounds the nucleoplasm of eukaryotic cells. It is composed of the nuclear lamina, nuclear pore complexes and two nuclear membranes. The space between the two membranes is called the nuclear intermembrane space.
Membrane Topology
Topology	Source	Annotation Type
Unknown	UniProt	Sequence Analysis
Assigned Ontology terms

Cellular Component	Nuclear Envelope (GO:0005635) Nucleus (GO:0005634) Transcription Export Complex 2 (GO:0070390)

Biology & Function

Description
Component of the SAC3-THP1 complex, which functions in transcription-coupled mRNA export from the nucleus to the cytoplasm. SAC3-THP1 functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), by association with components of the nuclear mRNA export machinery (MEX67-MTR2 and SUB2) in the nucleoplasm and the nucleoporin NUP1 at the nuclear basket. THP1 binds to RNA in vitro. {Experimental EvidencePubMed:11139493, Experimental EvidencePubMed:12411502, Experimental EvidencePubMed:12702719}.
Assigned Ontology terms

Biological Process	Cellular Bud Site Selection (GO:0000282) MRNA 3'-End Processing (GO:0031124) MRNA Export From Nucleus (GO:0006406) Nuclear MRNA Surveillance (GO:0071028) Poly(A)+ MRNA Export From Nucleus (GO:0016973) Positive Regulation Of Transcription By RNA Polymerase II (GO:0045944) Post-Transcriptional Tethering Of RNA Polymerase II Gene DNA At Nuclear Periphery (GO:0000973) Transcription Elongation By RNA Polymerase II Promoter (GO:0006368) Transcription-Coupled Nucleotide-Excision Repair (GO:0006283)
Molecular Function	Double-Stranded DNA Binding (GO:0003690) Protein-Containing Complex Binding (GO:0044877) RNA Binding (GO:0003723)

Protein-Protein Interactions

Interactions with Nuclear Envelope proteins (2 interactors)

Partner (NucEnvDB)		IntAct	Confidence score
P06704	Cell division control protein 31	EBI-1370592	0.76
P46674	Nuclear mRNA export protein SAC3	EBI-797734	0.88
Interactions with other proteins (17 interactors)

Partner (UniProt)		IntAct	Confidence score
P21576	Vacuolar protein sorting-associated protein 1	EBI-797734	0.35
P02994	Elongation factor 1-alpha (EF-1-alpha) (Eukaryotic elongation factor 1A) (eEF1A) (Translation elongation factor 1A)	EBI-7081522	0.40
Q02804	ADP-ribosylation factor-like protein 3 (Arf-like GTPase 3)	EBI-8230453	0.22
Q6WNK7	Transcription and mRNA export factor SUS1	EBI-1370393	0.78
Q02336	Transcriptional adapter 2	EBI-1370467	0.50
P53040	Transcription initiation factor TFIID subunit 6 (TBP-associated factor 6) (TBP-associated factor 60 kDa) (TAFII-60) (TAFII60)	EBI-1370499	0.35
Q03761	Transcription initiation factor TFIID subunit 12 (TAFII-61) (TAFII61) (TAFII-68) (TAFII68) (TBP-associated factor 12) (TBP-associated factor 61 kDa)	EBI-1370619	0.35
P50875	Transcription factor SPT20	EBI-1370619	0.35
P38811	Transcription-associated protein 1 (p400 kDa component of SAGA)	EBI-2124399	0.35
P10591	Heat shock protein SSA1 (Heat shock protein YG100)	EBI-3678586	0.35
P11484	Ribosome-associated molecular chaperone SSB1 (EC 3.6.4.10) (Cold-inducible protein YG101) (Heat shock protein SSB1) (Hsp70 chaperone Ssb)	EBI-3697051	0.35
P10592	Heat shock protein SSA2	EBI-3716418	0.35
Q05931	Iron-sulfur cluster biogenesis chaperone, mitochondrial (EC 3.6.4.10) (Heat shock protein SSQ1, mitochondrial) (Stress-seventy subfamily Q protein 1) (mtHSP70 homolog)	EBI-3720118	0.35
P40150	Ribosome-associated molecular chaperone SSB2 (EC 3.6.4.10) (Heat shock protein SSB2) (Hsp70 chaperone Ssb)	EBI-3722302	0.35
P32589	Heat shock protein homolog SSE1 (Chaperone protein MSI3)	EBI-3730594	0.35
P33416	Heat shock protein 78, mitochondrial	EBI-3747715	0.35
O94742	26S proteasome complex subunit SEM1	EBI-15970396	0.50

Cross-References

Database	Links
UNIPROT	Q08231 D6W1Z5
PDB	3T5V 4TRQ 5G5P 5L3T 5UBP
Pfam	PF01399
PROSITE	PS50250

Nuclear mRNA export protein THP1 (Saccharomyces cerevisiae S288C)

General Information

Protein Information

Structure Viewer (PDB: 5UBP)

Subcellular Location

Description Show

Position in the Nuclear Envelope

Membrane Topology

Assigned Ontology terms

Biology & Function

Description Hide

Assigned Ontology terms

Protein-Protein Interactions

Interactions with Nuclear Envelope proteins (2 interactors)

Interactions with other proteins (17 interactors)

Cross-References

Database

Links

Description

Description