Nuclear protein localization protein 4 - Saccharomyces cerevisiae S288C

Nuclear protein localization protein 4 (Saccharomyces cerevisiae S288C)

FASTA TEXT XML

Subcellular Location Biology & Function Interactions Cross-References

General Information

Protein Information
Protein Name	Nuclear protein localization protein 4
Accession Code	P33755
Gene	NPL4
Organism	Saccharomyces cerevisiae S288C (Taxonomy: 559292)
Part of Reference Proteome?	Yes
Sequence (Length: 580)
MLIRFRSKNGTHRVSCQENDLFGTVIEKLVGNLDPNADVDTFTVCEKPGQGIHAVSELADRTVMDLGLKHGDMLILNYSD KPANEKDGVNVEIGSVGIDSKGIRQHRYGPLRIKELAVDEELEKEDGLIPRQKSKLCKHGDRGMCEYCSPLPPWDKEYHE KNKIKHISFHSYLKKLNENANKKENGSSYISPLSEPDFRINKRCHNGHEPWPRGICSKCQPSAITLQQQEFRMVDHVEFQ KSEIINEFIQAWRYTGMQRFGYMYGSYSKYDNTPLGIKAVVEAIYEPPQHDEQDGLTMDVEQVKNEMLQIDRQAQEMGLS RIGLIFTDLSDAGAGDGSVFCKRHKDSFFLSSLEVIMAARHQTRHPNVSKYSEQGFFSSKFVTCVISGNLEGEIDISSYQ VSTEAEALVTADMISGSTFPSMAYINDTTDERYVPEIFYMKSNEYGITVKENAKPAFPVDYLLVTLTHGFPNTDTETNSK FVSSTGFPWSNRQAMGQSQDYQELKKYLFNVASSGDFNLLHEKISNFHLLLYINSLQILSPDEWKLLIESAVKNEWEESL LKLVSSAGWQTLVMILQESG

Structure Viewer (PDB: 6JWJ)

Subcellular Location

Description
Cytoplasm, perinuclear region. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes mainly at the nuclear periphery and the endoplasmic reticulum membrane.
Position in the Nuclear Envelope
Location	Location ID	Description
Nuclear Envelope	SL-0178	The nuclear envelope is a membrane system which surrounds the nucleoplasm of eukaryotic cells. It is composed of the nuclear lamina, nuclear pore complexes and two nuclear membranes. The space between the two membranes is called the nuclear intermembrane space.
Nuclear Membrane	SL-0182	The membrane surrounding the nucleus. This term is used when it is not known if the protein is found in or associated with the inner or outer nuclear membrane.
Perinuclear Region	SL-0198	The perinuclear region is the cytoplasmic region just around the nucleus.
Membrane Topology
Topology	Source	Annotation Type
Peripheral	UniProt	Sequence Analysis
Assigned Ontology terms

Cellular Component	Cdc48p-Npl4p-Vms1p AAA ATPase Complex (GO:0036266) Cytoplasm (GO:0005737) Doa10p Ubiquitin Ligase Complex (GO:0000837) Hrd1p Ubiquitin Ligase ERAD-L Complex (GO:0000839) Nuclear Membrane (GO:0031965) Nuclear Outer Membrane-Endoplasmic Reticulum Membrane Network (GO:0042175) Nucleus (GO:0005634) Perinuclear Region Of Cytoplasm (GO:0048471) Replisome (GO:0030894) RQC Complex (GO:1990112) VCP-NPL4-UFD1 AAA ATPase Complex (GO:0034098)

Biology & Function

Description
Substrate-recruiting cofactor of the CDC48-NPL4-UFD1 segregase (PubMed:31249135). Assists CDC48 in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation (PubMed:11739805, PubMed:11740563, PubMed:11847109). Involved in the import of nuclear- targeted proteins into the nucleus and the export of poly(A) RNA out of the nucleus (PubMed:8930904, PubMed:11733065). Required for the proteasome-dependent processing/activation of MGA2 and SPT23 transcription factors leading to the subsequent expression of OLE1 (PubMed:11733065). Regulates ubiquitin-mediated mitochondria protein degradation (PubMed:31249135). Involved in spindle disassembly probably by promoting the degradation of spindle assemby factors ASE1 and CDC5 at the end of mitosis (PubMed:14636562). {Experimental EvidencePubMed:11733065, Experimental EvidencePubMed:11739805, Experimental EvidencePubMed:11740563, Experimental EvidencePubMed:11847109, Experimental EvidencePubMed:14636562, Experimental EvidencePubMed:31249135, Experimental EvidencePubMed:8930904}.
Assigned Ontology terms

Biological Process	Cytoplasm Protein Quality Control By The Ubiquitin-Proteasome System (GO:0071629) DNA Replication Termination (GO:0006274) ER-Associated Misfolded Protein Catabolic Process (GO:0071712) Mitochondria-Associated Ubiquitin-Dependent Protein Catabolic Process (GO:0072671) Mitotic Spindle Disassembly (GO:0051228) MRNA Transport (GO:0051028) Negative Regulation Of Telomerase Activity (GO:0051974) Nonfunctional RRNA Decay (GO:0070651) Positive Regulation Of Protein Localization To Nucleus (GO:1900182) Proteasome-Mediated Ubiquitin-Dependent Protein Catabolic Process (GO:0043161) Protein Localization To Vacuole (GO:0072665) Protein Quality Control For Misfolded Or Incompletely Synthesized Proteins (GO:0006515) Retrograde Protein Transport, ER To Cytosol (GO:0030970) Ribosome-Associated Ubiquitin-Dependent Protein Catabolic Process (GO:1990116) Ubiquitin-Dependent ERAD Pathway (GO:0030433) Ubiquitin-Dependent Protein Catabolic Process (GO:0006511)
Molecular Function	Ubiquitin Binding (GO:0043130) Ubiquitin Protein Ligase Binding (GO:0031625)

Protein-Protein Interactions

Interactions with other proteins (23 interactors)

Partner (UniProt)		IntAct	Confidence score
P53044	Ubiquitin fusion degradation protein 1 (UB fusion protein 1) (Polymerase-interacting protein 3)	EBI-390843	0.93
P32467	Low-affinity glucose transporter HXT4 (Low-affinity glucose transporter LGT1)	EBI-792844	0.35
P16140	V-type proton ATPase subunit B (V-ATPase subunit B) (V-ATPase 57 kDa subunit) (Vacuolar proton pump subunit B)	EBI-794304	0.35
P02994	Elongation factor 1-alpha (EF-1-alpha) (Eukaryotic elongation factor 1A) (eEF1A) (Translation elongation factor 1A)	EBI-794304	0.35
P06169	Pyruvate decarboxylase isozyme 1 (EC 4.1.1.-) (EC 4.1.1.43) (EC 4.1.1.72) (EC 4.1.1.74) (Thiamine pyrophosphate-dependent 2-oxo-acid decarboxylase) (2ODC)	EBI-794304	0.35
P25694	Cell division control protein 48 (EC 3.6.4.6) (Cell division cycle protein 48) (Transitional endoplasmic reticulum ATPase homolog)	EBI-794304	0.91
Q04228	UBX domain-containing protein 2 (Secretion lowering protein 1)	EBI-805595	0.74
P41832	Protein BNI1 (Pointed projection formation protein 3) (Sensitive to high expression protein 5) (Synthetic lethal 39)	EBI-818477	0.27
Q02159	Ubiquitin-conjugating enzyme E2 7 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme 7) (Ubiquitin carrier protein) (Ubiquitin-conjugating enzyme E2-18 kDa) (Ubiquitin-protein ligase)	EBI-860287	0.00
P39081	Protein PCF11 (protein 1 of CF I)	EBI-861487	0.00
P28707	Co-chaperone protein SBA1	EBI-7671928	0.40
P53899	CDC48-associated ubiquitin-like/zinc finger protein 1 (CDC48-associated UBL/Zn-finger protein 1)	EBI-7872468	0.40
P47113	Mitotic check point protein BFA1 (Cell cycle arrest protein BFA1)	EBI-8221329	0.22
Q05787	ERAD-associated E3 ubiquitin-protein ligase component HRD3 (HMG-CoA reductase degradation protein 3)	EBI-1008463	0.35
Q08109	ERAD-associated E3 ubiquitin-protein ligase HRD1 (EC 2.3.2.27) (HMG-CoA reductase degradation protein 1) (RING-type E3 ubiquitin transferase HRD1)	EBI-1008463	0.53
P38307	Degradation in the endoplasmic reticulum protein 1	EBI-7894018	0.35
Q12743	DER1-like family member protein 1	EBI-7894121	0.35
P54860	E4 ubiquitin-protein ligase UFD2 (EC 2.3.2.27) (RING-type E3 ubiquitin transferase UFD2) (Ubiquitin conjugation factor E4) (Ubiquitin fusion degradation protein 2) (UB fusion protein 2)	EBI-2343183	0.55
Q05468	Ribosome quality control complex subunit 1	EBI-6554533	0.35
Q12532	Ribosome quality control complex subunit 2 (Translation-associated element 2)	EBI-6554563	0.35
Q04311	Protein VMS1 (VCP/CDC48-associated mitochondrial stress-responsive protein 1)	EBI-9510937	0.40
Q03935	AP-1-like transcription factor YAP6	EBI-16251296	0.00
P04838	Polyubiquitin [Cleaved into: Ubiquitin-related; Ubiquitin]	EBI-21915226	0.35

Cross-References

Database	Links
UNIPROT	P33755 D6VQG6
PDB	6JWH 6JWI 6JWJ 6OA9 6OAA
Pfam	PF05021 PF05020
PROSITE	PS50249

NucEnvDB

NucEnvDB

A database of Nuclear Envelope Proteins and their Interactions

Nuclear protein localization protein 4 (Saccharomyces cerevisiae S288C)

General Information

Protein Information

Structure Viewer (PDB: 6JWJ)

Subcellular Location

Description

Position in the Nuclear Envelope

Membrane Topology

Assigned Ontology terms

Biology & Function

Description

Assigned Ontology terms

Protein-Protein Interactions

Interactions with other proteins (23 interactors)

Cross-References

Database

Links

Nuclear protein localization protein 4 (Saccharomyces cerevisiae S288C)

General Information

Protein Information

Structure Viewer (PDB: 6JWJ)

Subcellular Location

Description Show

Position in the Nuclear Envelope

Membrane Topology

Assigned Ontology terms

Biology & Function

Description Hide

Assigned Ontology terms

Protein-Protein Interactions

Interactions with other proteins (23 interactors)

Cross-References

Database

Links

Description

Description