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FACULTY OF BIOLOGY

Department of Biochemistry-
Molecular Biology
Laboratory of Protein Structure and Function

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    Publications

    1. Serban D, Arcineigas S. F., Vorgias C. E., Thomas G. J Jr. (2003) Structure and dynamics of the DNA-binding protein HU of B. stearothermophilus investigated by Raman and ultraviolet-resonance Raman spectroscopy. Protein Sci 12(4): 861-870.pdf
    2. Papanikolau Y., Vorgias C. E. and Petratos K. (2003) De novo purification scheme and crystallization conditions yield high-resolution structures of chitinase A and its complex with the inhibitor allosamidin. Acta Crystallogr. D 59: 400-403.pdf
    3. Christodoulou E., Rypniewski W. and Vorgias C. E. (2003) High resolution X-ray structure of the DNA-binding protein HU from the hyper-thermophilic Thermotoga maritima and the determinants of its thermostability. Extremophiles 7, 111-122.pdf
    4. Bernard M., Mouyna I., Dubreucq G., Debeaupuis J.-P., Fontaine T., Vorgias C. E., Fuglsang C. and Latgι J-P. (2002) Characterization of a cell-wall acid phosphatase (PhoAp) in Aspergillus fumigatus. Microbiology 148, 2819-2829.pdf
    5. Christodoulou E. and Vorgias C. E. (2002) Understanding heterologous protein overproduction under the T7 promoter: A practical exercise. Biochemical and Mol. Biol. Education 30, 189-191.pdf
    6. Christodoulou E. and Vorgias CE. (2002) The thermostability of DNA-binding protein HU from mesophilic, thermophilic and extreme thermophilic bacteria. Extremophiles 6, 21-31.pdf
    7. Raves M. L., Doreleijers J., Vis H., Vorgias C. E., Wilson K. S. and Kaptein R. (2001) Joint refinement as a tool for thorough comparison between NMR and X-ray data and structures of HU protein. J. Biomolecular NMR 21, 235-248.pdf
    8. Prog G., Vorgias C. E. and Oppenheim, A. B. (2001) Conservation of structural elements and catalytic mechanism in the chitinolytic enzymes from Serratia marcescens. Chitin Enzymology, 2001. Ancona pp. 351-360.pdf
    9. Papanikolau Y., Prag G., Tavlas G., Vorgias C. E., Oppenheim A. B. and Petratos K. (2001) High resolution structural analyses of mutant Chitinase A complexes with substrates provide new insight into the mechanism of catalysis. Biochemistry 40, 11338-11343.pdf
    10. Christodoulou E., Duffner F. and Vorgias C. E. (2001) Overexpression, purification and characterization of a thermostable Chitinase (Chi40) from Streptomyces thermoviolaceus OPC-520. Protein Expr. Purif. 23, 97-105.pdf
    11. Lonhienne T. G. A., Zoidakis J., Vorgias C. E, Feller G., Gerday C., and Bouriotis V. (2001) Modular structure, local flexibility and cold-activity of a novel chitobiase from a psychrophilic antarctic bacterium J. Mol. Biol. 310, 291-297.pdf
    12. Lonhienne T. G. A., Mavromattis C., Vorgias C. E, Buchon L., Gerday C. and Bouriotis V. (2001) Cloning, sequences, and characterization of two chitinase genes from the antarctic Arthrobacter sp. strain TAD20: Isolation and partial characterization of the enzymes. J. Bacteriology 183, 1773-1779.pdf
    13. Fontaine T., Simenel C., Dubreucq G., Adam O., Delepierre M., Lemoine J., Vorgias C. E., Diaquin M. and Latgι J-P. (2000) Molecular organization of the alkali-insoluble fraction of Aspergillus fumigatus cell wall. J. Biol. Chem. 275, 27594-27607.pdf
    14. Prag G., Papanikolau Y., Tavlas G., Vorgias C. E., Petratos K. and Oppenheim A. B. (2000) Structures of chitobiase mutants complexed with the substrate di-N-acetyl-D-glucosamine: the catalytic role of the conserved acidic pair, aspartate-539 and glutamate-540. J. Mol. Biol. 300, 611-617.pdf
    15. Boehlke K., Pisani F. M, Vorgias C. E., Frey B., Sobek H., Rossi M. and Antranikian G. (2000) PCR performance of the B-type DNA polymerase from the thermophilic euryarchaeon Thermococcus aggregans improved by mutations in the Y-GG/A motif. Nucleic Acids Res. 28, 3910-3917.pdf
    16. Vorgias C. E. and Antranikian G. (2000) Glycosyl Hydrolases from Extremophiles. In Glycomicrobiology, pp313-339 (ed. by Doyle) Kluwer Academic/Plenum Publishers, N. York.pdf
    17. Vis H, Vorgias C. E., Wilson K. S., Kaptein R. and Boelens R. (1998) Mobility of NH bonds in DNA-binding protein HU of Bacillus stearothermophilus from reduced spectral density mapping analysis at multiple NMR fields. J. Biomolecular NMR 11, 265-277.pdf
    18. Christodoulou E. and Vorgias C. E. (1998) Cloning, overproduction, purification and crystallisation of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima. Acta Cryst. D54, 1043-1045.pdf
    19. Vorgias C. E. (1997) Structural basis of chitin hydrolysis in bacteria. Advances in Chitin Society Vol II, pp. 176-187.pdf
    20. Vorgias C. E. and Petratos K. (1997) 3D-structure and enzymatic mechanism of chitinase A from the chitinolytic soil bacterium Serratia marcescens. Chapter in Chitin Handbook Chapter in Chitin Handbook (Muzzarelli and Peter eds.) ISBN 88-86889-01-1.pdf
    21. Vorgias C. E. (1997) Overproduction of the recombinant chitinase A from Serratia marcescens in E. coli: purification, biochemical and biophysical characterisation. Chapter in Chitin Handbook (Muzzarelli and Peter eds.) ISBN 88-86889-01-1.pdf
    22. Drouillard S., Armand S., Davies G., Vorgias C. E. and Henrissat B. (1997) Serratia marcescens chitobiase is a retaining glycosidase utilising substrate acetamido group participation. Biochem. J. 328, 945-949.pdf
    23. Joergensen S., Vorgias C. E. and Antranikian G. (1997) Cloning, Sequencing, Characterization, and Expression of an Extracellular α-Amylase from the Hyperthermophilic Archaeon Pyrococcus furiosus in Escherichia coli and Bacillus subtilis. J. Biol. Chem. 272, 16335-16342.
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    24. Spindler-Bath M., Blattner R., Vorgias C. E. and Spindler K-D. (1998) Inhibition of two family 18 chitinases by various allosamidin derivatives. Pestic. Sci. 52, 47-52.pdf
    25. Chernin L. S., De la Fuente., Sobolev V., Haran S., Vorgias C. E., Oppenheim A. B. and Chet I. (1997) Molecular cloning, structural analysis and expression in Escherichia coli of a chitinase gene from Enterobacter agglomerans. Appl. Env. Microbiology 63, 834-839.pdf
    26. Fernandes M. J. G., Leclerc D., Henrissat. B., Vorgias C. E., Gravel R., Hechtman P. and Kalpan F. (1997) Identification of candidate active site residues in lysosomal β-Hexosaminidase A. J. Biol. Chem. 272, 814-820.pdf
    27. Tews I., Wilson K. S. and Vorgias C. E. (1996) Structural studies on N-acetylglucosaminidase enzyme-inhibitor complexes. In Chitin Enzymology (Muzzarelli, R. A. A. ed.) Vol 2, pp 213-225.pdf
    28. Perrakis A., Tews I. and Vorgias C. E. (1996) Structural aspects on the catalytic mechanism of Chitinases, Hevamine and Chitobiases: “Far away and yet so close?” In Chitin Enzymology (Muzzarelli, R. A. A. ed.) Vol 2, pp 109-122.pdf
    29. Vorgias C. E., Perrakis A. and Tews I. (1996) Structure-Function studies on the chitinolytic enzymes of Serratia marcescens chitinase and chitobiase. Annals of the New York Academy of Science. 799, 190-192.pdf
    30. Kanellopoulos P. N., Pavlou K., Perrakis A., Aganian B., Vorgias C. E., Mavrommatis C., Soufi M., Tucker P. A. and Hamodrakas S. J. (1996) The crystal structure of the complexes of Concanavalin A with 4’-nitrophenyl-a-D-mannopyranoside and 4’-nitrophenyl-a-D-glucopyranoside. J. Struct. Biol. 116, 345-355.pdf