FACULTY OF BIOLOGY
Department of Biochemistry-
Molecular Biology
Laboratory of Protein Structure and Function
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Publications
Perrakis A., Ouzounis C. Wilson K. S. and Vorgias C. E. (1996) Implications of the 3-D structure determination of family 18 chitinases. Similarities with FnIII domains, oviductal proteins and narbonins. Advances in Chitin Science Vol. 1. pp 34-41.
Tews I., Wilson K. S. and Vorgias C. E. (1996) Enzymatic mechanism of N-acetylglucosaminidase revealed by structural studies on enzyme substrate-inhibitor complexes. Advances in Chitin Science Vol. 1. pp. 26-33.
Tews I., Vincentelli R. and Vorgias C. E. (1996) N-acetylglucosaminidase (chitobiase) from
Serratia marcescens
: gene sequence, and protein production and purification in
Escherichia coli
. Gene 170, 63-67.
Vis H., Vageli O., Nagel J., Vorgias C. E., Wilson K. S., Kaptein R. and Boelens R. (1996) NMR study of the interaction of the HU protein from
Bacillus stearothermophilus
with DNA. Magnetic Resonance in Chemistry 34, 81-86.
Tews I., Perrakis A., Dauter Z., Oppenheim A. B., Wilson K. S. and Vorgias C. E. (1996) Bacterial chitobiase structure provides insight into catalytic mechanism and the basis of Tay-Sachs disease. Nature Structural Biology 3, 638-648.
Boelens R., Vis H., Vorgias C. E., Wilson K. S. and Kaptein R. (1996) Structure and dynamics of the DNA binding protein HU from
Bacillus stearothermophilus
by NMR spectroscopy. Biopolymers 40, 553-559.
Sikorski M. M., Topunov A. F., Strozycki P. M., Vorgias C. E., Wilson K. S. and Legoski A. B. (1995) Cloning and expression of plant leghemoglobin cDNA of
Lupinus luteus
in
Escherichia coli
and purification of the recombinant protein. Plant Science 108, 109-117.
Sitrit Y., Vorgias C. E., Chet I. and Oppenheim A. B. (1995) Cloning and primary structure of the
chi
A gene from
Aeromonas caviae.
J. Bacteriology 177, 4187-4189.
Vis H., Mariani M., Vorgias C. E., Wilson K. S., Kaptein R. and Boelens R. (1995) Solution structure of the HU Protein from
Bacillus stearothermophilus.
J. Mol. Biol. 254, 692-703.
Perrakis A., Tews I., Dauter Z., Oppenheim, A. B., Chet I., Wilson K. S. and Vorgias C. E. (1994) Crystal structure of a bacterial chitinase at 2.3 Å resolution. Structure 2, 1169-1180.
Tews I, Vincentelli R., Perrakis A., Dauter Z., Wilson K. S. and Vorgias C. E. (1994) Primary and 3D structure of chitobiase from
Serratia marcescens
. In Chitin World (eds Karnicki, Z.S., Wojtasz-Pajak, A., Brzeski, M.M. and Bykowski, P.J.) pp 415-423.
Perrakis A., Tews I., Dauter Z., Wilson K. S. and Vorgias C. E. (1994) X-ray structure analysis of Chitinase A from
Serratia marcescens
. In Chitin World (eds Karnicki, Z.S., Wojtasz-Pajak, A., Brzeski, M.M. and Bykowski, P.J.) pp 408-415.
Vis H., Boelens R., Mariani M., Stroop R., Vorgias C. E., Wilson K. S. and Kaptein R. (1994)
1
H,
13
C and
15
N resonance assignments and secondary structure analysis of the HU protein from
Bacillus stearothermophilus
using two- and three-dimensional double- and triple-resonance heteronuclear magnetic resonance spectroscopy. Biochemistry 33, 14858-14870.
Rypniewski W. R., Perrakis A., Vorgias C. E. and Wilson K. S. (1994) Evolutionary divergence and conservation of trypsin. Protein Eng. 7, 57-64.
Perrakis A., Wilson K. S., Chet. I., Oppenheim, A. B. and Vorgias C. E. (1993) Phylogenetic relatioships of chitinases. In Chitin Enzymology (Muzzarelli, R. A. A. ed.) pp 217-232.
Vorgias C. E., Tews I., Perrakis A., Wilson K. S. and Oppenheim, A.
B. (1993) Purification and characterisation of the recombinant chitin degrading enzymes chitinase and chitobiase from
Serratia marcescens
. In Chitin Enzymology (Muzzarelli, R. A. A. ed.) pp 417-422.
Sayers Z., Brouillon P., Vorgias C. E., Nolting H. F., Hermes C. and Koch M. H. J. (1993) Cloning and expression of
Saccharomyces cerevisiae
copper-metallothionein gene in
Escherichia coli
and characterisation of the recombinant protein. Eur. J. Biochem. 212, 521-528.
Tews I., Dauter Z., Oppenheim A. B. and Vorgias C. E. (1992) Crystallisation of recombinant chitobiase from
Serratia marcescens.
J. Mol. Biol. 228, 696-697.
Vorgias C. E., Kingswell A. J., Dauter Z. and Oppenheim A. B. (1992) Crystallisation of recombinant chitinase from the cloned
chi
A gene of
Serratia marcescens.
J. Mol. Biol. 226, 897-898.
Padas M. P., Wilson K. S. and Vorgias C. E. (1992) The DNA-binding protein HU from mesophilic and thermophilic bacilli: gene cloning, overproduction and purification. Gene 117, 39-44.
Vorgias C. E. and Wilson K. S. (1991) A rapid method for the purification of recombinant integration host factor. Protein Expr. Purif. 2, 317-320.
Vorgias C. E., Lemaire H-G., Wilson K. S. (1991) Overexpression and purification of the galactose operon enzymes from
E. coli
. Protein Expr. Purif. 2, 330-338.
Vorgias C. E., Kingswell A. J., Dauter Z., Wilson K. S. (1991) Cloning, overexpression, purification and crystallisation of ribosomal protein L9 from
Bacillus stearothermophilus.
FEBS Lett. 286, 204-208.
Wilson K. S., Vorgias C. E., Tanaka I., White W. S. and Kimura M. (1990) The thermostability of DNA binding protein HU from thermophilic and mesophilic
bacilli
. Protein Engineering 4, 11-22.
Haubruck H., Prange R., Vorgias C. E. and Gallwitz, D. (1989) The
ras
-related mouse
ypt1
protein can functionally replace the YPT1 gene product in yeast EMBO J. 8, 1427-1432.
Gallwitz D., Haubruck H., Molenaar C., Prange R., Putzicha M., Schmitt H.-D., Vorgias C. E. and Wagner P. (1988) Structural and functional analysis of
ypt
proteins, a family of
ras
-related nucleotide-binding proteins in eucaryotic cells. In: The Guanine - Nucleotide Binding Proteins, Common Structural and Functional Properties, Edited by L. Bosch, B. Kraal and A. Parmeggiani, NATO ASI Series, Life Sciences Vol. 165, 257-264.
Vorgias C. E., Peridis G. A., Traub P. and Sekeris C. E. (1988) Colchicine,
colcemide and cytochalasin-b do not affect translocation of the glucocorticoid hormone-receptor in rat thymocytes or Ehrlich Ascites cells. Bioscience Reports 8, 193-197.
Kuehn S., Vorgias C. E. and Traub P. (1987) Interaction
in vitro
of non-epithelial intermediate proteins with supercoiled plasmid DNA. J. Cell Sci. 87, 543-554.
Zimmermann H.-P., Plagens U., Vorgias C. E. and Traub P. (1986) Changes in the organization of non-epithelial intermediate filaments induced by triethyl lead chloride. Exp. Cell Res. 167, 360-368.
Vorgias C. E. and Traub P. (1986) Efficient degradation
in vitro
of all intermediate filament subunits proteins by the Ca
2+
-activated neutral thiol proteinase from Ehrlich Ascite Tumor cells and procine kidney. Bioscience Reports 6, 57-64.
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