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FACULTY OF BIOLOGY

Department of Biochemistry-
Molecular Biology
Laboratory of Protein Structure and Function

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    Publications

    1. Perrakis A., Ouzounis C. Wilson K. S. and Vorgias C. E. (1996) Implications of the 3-D structure determination of family 18 chitinases. Similarities with FnIII domains, oviductal proteins and narbonins. Advances in Chitin Science Vol. 1. pp 34-41.pdf
    2. Tews I., Wilson K. S. and Vorgias C. E. (1996) Enzymatic mechanism of N-acetylglucosaminidase revealed by structural studies on enzyme substrate-inhibitor complexes. Advances in Chitin Science Vol. 1. pp. 26-33.pdf
    3. Tews I., Vincentelli R. and Vorgias C. E. (1996) N-acetylglucosaminidase (chitobiase) from Serratia marcescens: gene sequence, and protein production and purification in Escherichia coli. Gene 170, 63-67.pdf
    4. Vis H., Vageli O., Nagel J., Vorgias C. E., Wilson K. S., Kaptein R. and Boelens R. (1996) NMR study of the interaction of the HU protein from Bacillus stearothermophilus with DNA. Magnetic Resonance in Chemistry 34, 81-86.pdf
    5. Tews I., Perrakis A., Dauter Z., Oppenheim A. B., Wilson K. S. and Vorgias C. E. (1996) Bacterial chitobiase structure provides insight into catalytic mechanism and the basis of Tay-Sachs disease. Nature Structural Biology 3, 638-648.pdf
    6. Boelens R., Vis H., Vorgias C. E., Wilson K. S. and Kaptein R. (1996) Structure and dynamics of the DNA binding protein HU from Bacillus stearothermophilus by NMR spectroscopy. Biopolymers 40, 553-559.pdf
    7. Sikorski M. M., Topunov A. F., Strozycki P. M., Vorgias C. E., Wilson K. S. and Legoski A. B. (1995) Cloning and expression of plant leghemoglobin cDNA of Lupinus luteus in Escherichia coli and purification of the recombinant protein. Plant Science 108, 109-117.pdf
    8. Sitrit Y., Vorgias C. E., Chet I. and Oppenheim A. B. (1995) Cloning and primary structure of the chiA gene from Aeromonas caviae. J. Bacteriology 177, 4187-4189.pdf
    9. Vis H., Mariani M., Vorgias C. E., Wilson K. S., Kaptein R. and Boelens R. (1995) Solution structure of the HU Protein from Bacillus stearothermophilus. J. Mol. Biol. 254, 692-703.pdf
    10. Perrakis A., Tews I., Dauter Z., Oppenheim, A. B., Chet I., Wilson K. S. and Vorgias C. E. (1994) Crystal structure of a bacterial chitinase at 2.3 Å resolution. Structure 2, 1169-1180.pdf
    11. Tews I, Vincentelli R., Perrakis A., Dauter Z., Wilson K. S. and Vorgias C. E. (1994) Primary and 3D structure of chitobiase from Serratia marcescens. In Chitin World (eds Karnicki, Z.S., Wojtasz-Pajak, A., Brzeski, M.M. and Bykowski, P.J.) pp 415-423.pdf
    12. Perrakis A., Tews I., Dauter Z., Wilson K. S. and Vorgias C. E. (1994) X-ray structure analysis of Chitinase A from Serratia marcescens. In Chitin World (eds Karnicki, Z.S., Wojtasz-Pajak, A., Brzeski, M.M. and Bykowski, P.J.) pp 408-415.pdf
    13. Vis H., Boelens R., Mariani M., Stroop R., Vorgias C. E., Wilson K. S. and Kaptein R. (1994) 1H, 13C and 15N resonance assignments and secondary structure analysis of the HU protein from Bacillus stearothermophilus using two- and three-dimensional double- and triple-resonance heteronuclear magnetic resonance spectroscopy. Biochemistry 33, 14858-14870.pdf
    14. Rypniewski W. R., Perrakis A., Vorgias C. E. and Wilson K. S. (1994) Evolutionary divergence and conservation of trypsin. Protein Eng. 7, 57-64.pdf
    15. Perrakis A., Wilson K. S., Chet. I., Oppenheim, A. B. and Vorgias C. E. (1993) Phylogenetic relatioships of chitinases. In Chitin Enzymology (Muzzarelli, R. A. A. ed.) pp 217-232.pdf
    16. Vorgias C. E., Tews I., Perrakis A., Wilson K. S. and Oppenheim, A. B. (1993) Purification and characterisation of the recombinant chitin degrading enzymes chitinase and chitobiase from Serratia marcescens. In Chitin Enzymology (Muzzarelli, R. A. A. ed.) pp 417-422.pdf
    17. Sayers Z., Brouillon P., Vorgias C. E., Nolting H. F., Hermes C. and Koch M. H. J. (1993) Cloning and expression of Saccharomyces cerevisiae copper-metallothionein gene in Escherichia coli and characterisation of the recombinant protein. Eur. J. Biochem. 212, 521-528.pdf
    18. Tews I., Dauter Z., Oppenheim A. B. and Vorgias C. E. (1992) Crystallisation of recombinant chitobiase from Serratia marcescens. J. Mol. Biol. 228, 696-697.pdf
    19. Vorgias C. E., Kingswell A. J., Dauter Z. and Oppenheim A. B. (1992) Crystallisation of recombinant chitinase from the cloned chiA gene of Serratia marcescens. J. Mol. Biol. 226, 897-898.pdf
    20. Padas M. P., Wilson K. S. and Vorgias C. E. (1992) The DNA-binding protein HU from mesophilic and thermophilic bacilli: gene cloning, overproduction and purification. Gene 117, 39-44.
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    21. Vorgias C. E. and Wilson K. S. (1991) A rapid method for the purification of recombinant integration host factor. Protein Expr. Purif. 2, 317-320.pdf
    22. Vorgias C. E., Lemaire H-G., Wilson K. S. (1991) Overexpression and purification of the galactose operon enzymes from E. coli. Protein Expr. Purif. 2, 330-338.pdf
    23. Vorgias C. E., Kingswell A. J., Dauter Z., Wilson K. S. (1991) Cloning, overexpression, purification and crystallisation of ribosomal protein L9 from Bacillus stearothermophilus. FEBS Lett. 286, 204-208.pdf
    24. Wilson K. S., Vorgias C. E., Tanaka I., White W. S. and Kimura M. (1990) The thermostability of DNA binding protein HU from thermophilic and mesophilic bacilli. Protein Engineering 4, 11-22.pdf
    25. Haubruck H., Prange R., Vorgias C. E. and Gallwitz, D. (1989) The ras-related mouse ypt1 protein can functionally replace the YPT1 gene product in yeast EMBO J. 8, 1427-1432.pdf
    26. Gallwitz D., Haubruck H., Molenaar C., Prange R., Putzicha M., Schmitt H.-D., Vorgias C. E. and Wagner P. (1988) Structural and functional analysis of ypt proteins, a family of ras-related nucleotide-binding proteins in eucaryotic cells. In: The Guanine - Nucleotide Binding Proteins, Common Structural and Functional Properties, Edited by L. Bosch, B. Kraal and A. Parmeggiani, NATO ASI Series, Life Sciences Vol. 165, 257-264.pdf
    27. Vorgias C. E., Peridis G. A., Traub P. and Sekeris C. E. (1988) Colchicine, colcemide and cytochalasin-b do not affect translocation of the glucocorticoid hormone-receptor in rat thymocytes or Ehrlich Ascites cells. Bioscience Reports 8, 193-197.pdf
    28. Kuehn S., Vorgias C. E. and Traub P. (1987) Interaction in vitro of non-epithelial intermediate proteins with supercoiled plasmid DNA. J. Cell Sci. 87, 543-554.pdf
    29. Zimmermann H.-P., Plagens U., Vorgias C. E. and Traub P. (1986) Changes in the organization of non-epithelial intermediate filaments induced by triethyl lead chloride. Exp. Cell Res. 167, 360-368.pdf
    30. Vorgias C. E. and Traub P. (1986) Efficient degradation in vitro of all intermediate filament subunits proteins by the Ca2+-activated neutral thiol proteinase from Ehrlich Ascite Tumor cells and procine kidney. Bioscience Reports 6, 57-64.pdf