Cyclin-G-associated kinase - Homo sapiens

Cyclin-G-associated kinase (Homo sapiens)

FASTA TEXT XML

Subcellular Location Biology & Function Interactions Cross-References

General Information

Protein Information
Protein Name	Cyclin-G-associated kinase
Accession Code	O14976
Gene	GAK
Organism	Homo sapiens \| Human (Taxonomy: 9606)
Part of Reference Proteome?	Yes
Sequence (Length: 1311)
MSLLQSALDFLAGPGSLGGASGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNR AIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQ HMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPI GEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQEIAAAR NVNPKSPITELLEQNGGYGSATLSRGPPPPVGPAGSGYSGGLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSV ANYAKGDLDISYITSRIAVMSFPAEGVESALKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSRFHNRVSECGWAARRAP HLHTLYNICRNMHAWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMC DMVAEEPITPHSKPILVRAVVMTPVPLFSKQRSGCRPFCEVYVGDERVASTSQEYDKMRDFKIEDGKAVIPLGVTVQGDV LIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAP PWENSSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAGAGSPEAEPTDSDSPPSSSADASRFLHTLDWQEE KEAETGAENASSKESESALMEDRDESEVSDEGGSPISSEGQEPRADPEPPGLAAGLVQQDLVFEVETPAVLPEPVPQEDG VDLLGLHSEVGAGPAVPPQACKAPSSNTDLLSCLLGPPEAASQGPPEDLLSEDPLLLASPAPPLSVQSTPRGGPPAAADP FGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSAPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAW TETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQ TSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKT IAEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAPEQVKKHYRRAVLAVHPDKAA GQPYEQHAKMIFMELNDAWSEFENQGSRPLF

Structure Viewer (PDB: 4C59)

Subcellular Location

Description
Cytoplasm, perinuclear region {Experimental EvidencePubMed:10625686}. Golgi apparatus, trans-Golgi network {Experimental EvidencePubMed:10625686}. Cell junction, focal adhesion {ECO:0000305\|PubMed:10625686}. Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
Position in the Nuclear Envelope
Location	Location ID	Description
Perinuclear Region	SL-0198	The perinuclear region is the cytoplasmic region just around the nucleus.
Membrane Topology
Topology	Source	Annotation Type
Unknown	UniProt	Sequence Analysis
Assigned Ontology terms

Cellular Component	Cytoplasm (GO:0005737) Cytosol (GO:0005829) Focal Adhesion (GO:0005925) Golgi Apparatus (GO:0005794) Intracellular Membrane-Bounded Organelle (GO:0043231) Membrane (GO:0016020) Perinuclear Region Of Cytoplasm (GO:0048471) Presynapse (GO:0098793) Vesicle (GO:0031982)

Biology & Function

Description
Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. {Experimental EvidencePubMed:10625686}.
Assigned Ontology terms

Biological Process	Cell Cycle (GO:0007049) Chaperone Cofactor-Dependent Protein Refolding (GO:0051085) Clathrin Coat Disassembly (GO:0072318) Clathrin-Coated Pit Assembly (GO:1905224) Clathrin-Dependent Endocytosis (GO:0072583) Endoplasmic Reticulum Organization (GO:0007029) Golgi Organization (GO:0007030) Golgi To Lysosome Transport (GO:0090160) Negative Regulation Of Neuron Projection Development (GO:0010977) Protein Localization To Golgi Apparatus (GO:0034067) Protein Localization To Plasma Membrane (GO:0072659) Protein Phosphorylation (GO:0006468) Receptor-Mediated Endocytosis (GO:0006898) Synaptic Vesicle Uncoating (GO:0016191)
Molecular Function	ATP Binding (GO:0005524) Chaperone Binding (GO:0051087) Clathrin Binding (GO:0030276) Cyclin Binding (GO:0030332) Protein Serine Kinase Activity (GO:0106310) Protein Serine/Threonine Kinase Activity (GO:0004674)

Protein-Protein Interactions

Interactions with Nuclear Envelope proteins (9 interactors)

Partner (NucEnvDB)		IntAct	Confidence score
O14966	Ras-related protein Rab-7L1	EBI-9247728	0.35
P63005	Platelet-activating factor acetylhydrolase IB subunit beta	EBI-11041417	0.35
O15027	Protein transport protein Sec16A	EBI-11083608	0.35
O75146	Huntingtin-interacting protein 1-related protein	EBI-11150908	0.53
P55735	Protein SEC13 homolog	EBI-11150908	0.35
Q27J81	Inverted formin-2	EBI-11150908	0.35
Q9UM54	Unconventional myosin-VI	EBI-11150908	0.35
Q07912	Activated CDC42 kinase 1	EBI-11150908	0.35
Q14677	Clathrin interactor 1	EBI-11150908	0.35
Interactions with other proteins (96 interactors)

Partner (UniProt)		IntAct	Confidence score
O15169	Axin-1 (Axis inhibition protein 1) (hAxin)	EBI-731365	0.00
O60763	General vesicular transport factor p115 (Protein USO1 homolog) (Transcytosis-associated protein) (TAP) (Vesicle-docking protein)	EBI-759358	0.37
P10275	Androgen receptor (Dihydrotestosterone receptor) (Nuclear receptor subfamily 3 group C member 4)	EBI-1632203	0.37
Q9Q2G4	V-1 protease	EBI-6174875	0.35
Q9NQ11	Polyamine-transporting ATPase 13A2 (EC 7.6.2.-)	EBI-6377262	0.51
Q5S007	Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (EC 3.6.5.-) (Dardarin)	EBI-9247177	0.79
P11142	Heat shock cognate 71 kDa protein (EC 3.6.4.10) (Heat shock 70 kDa protein 8) (Lipopolysaccharide-associated protein 1) (LAP-1) (LPS-associated protein 1)	EBI-9655962	0.69
Q9UL15	BAG family molecular chaperone regulator 5 (BAG-5) (Bcl-2-associated athanogene 5)	EBI-9655962	0.35
Q38SD2	Leucine-rich repeat serine/threonine-protein kinase 1 (EC 2.7.11.1)	EBI-9659083	0.44
Q76MZ3	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PP2A subunit A isoform PR65-alpha) (PP2A subunit A isoform R1-alpha)	EBI-10991736	0.35
A2AUM9	Centrosomal protein of 152 kDa (Cep152)	EBI-10994361	0.35
P10126	Elongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor Tu) (EF-Tu) (Eukaryotic elongation factor 1 A-1) (eEF1A-1)	EBI-11048962	0.35
G3X972	Sec24-related gene family, member C (S. cerevisiae)	EBI-11079358	0.35
P09497	Clathrin light chain B (Lcb)	EBI-11081190	0.35
Q9NYZ3	G2 and S phase-expressed protein 1 (GTSE-1) (Protein B99 homolog)	EBI-11081743	0.35
Q13492	Phosphatidylinositol-binding clathrin assembly protein (Clathrin assembly lymphoid myeloid leukemia protein)	EBI-11082344	0.35
Q00610	Clathrin heavy chain 1 (Clathrin heavy chain on chromosome 17) (CLH-17)	EBI-11082478	0.35
Q9Z1Z0	General vesicular transport factor p115 (Protein USO1 homolog) (Transcytosis-associated protein) (TAP) (Vesicle-docking protein)	EBI-11112182	0.35
P15735	Phosphorylase b kinase gamma catalytic chain, liver/testis isoform (PHK-gamma-LT) (PHK-gamma-T) (EC 2.7.11.19) (PSK-C3) (Phosphorylase kinase subunit gamma-2)	EBI-11135895	0.35
P51805	Plexin-A3 (Plexin-4) (Semaphorin receptor SEX)	EBI-11150908	0.35
Q96CW1	AP-2 complex subunit mu (AP-2 mu chain) (Adaptin-mu2) (Adaptor protein complex AP-2 subunit mu) (Adaptor-related protein complex 2 subunit mu) (Clathrin assembly protein complex 2 mu medium chain) (Clathrin coat assembly protein AP50) (Clathrin coat-associated protein AP50) (HA2 50 kDa subunit) (Plasma membrane adaptor AP-2 50 kDa protein)	EBI-11150908	0.35
Q9H2Y7	Zinc finger protein 106 (Zfp-106) (Zinc finger protein 474)	EBI-11150908	0.35
Q5TB80	Centrosomal protein of 162 kDa (Cep162) (Protein QN1 homolog)	EBI-11150908	0.35
Q8TEH3	DENN domain-containing protein 1A (Connecdenn 1) (Connecdenn) (Protein FAM31A)	EBI-11150908	0.35
Q8N684	Cleavage and polyadenylation specificity factor subunit 7 (Cleavage and polyadenylation specificity factor 59 kDa subunit) (CPSF 59 kDa subunit) (Cleavage factor Im complex 59 kDa subunit) (CFIm59) (Pre-mRNA cleavage factor Im 59 kDa subunit)	EBI-11150908	0.35
Q9Y496	Kinesin-like protein KIF3A (Microtubule plus end-directed kinesin motor 3A)	EBI-11150908	0.35
Q14789	Golgin subfamily B member 1 (372 kDa Golgi complex-associated protein) (GCP372) (Giantin) (Macrogolgin)	EBI-11150908	0.35
Q2M2I8	AP2-associated protein kinase 1 (EC 2.7.11.1) (Adaptor-associated kinase 1)	EBI-11150908	0.35
Q8N556	Actin filament-associated protein 1 (110 kDa actin filament-associated protein) (AFAP-110)	EBI-11150908	0.35
O43175	D-3-phosphoglycerate dehydrogenase (3-PGDH) (EC 1.1.1.95) (2-oxoglutarate reductase) (EC 1.1.1.399) (Malate dehydrogenase) (EC 1.1.1.37)	EBI-11150908	0.35
P47756	F-actin-capping protein subunit beta (CapZ beta)	EBI-11150908	0.35
P27482	Calmodulin-like protein 3 (CaM-like protein) (CLP) (Calmodulin-related protein NB-1)	EBI-11150908	0.35
P53992	Protein transport protein Sec24C (SEC24-related protein C)	EBI-11150908	0.35
Q9UHB6	LIM domain and actin-binding protein 1 (Epithelial protein lost in neoplasm)	EBI-11150908	0.35
P49757	Protein numb homolog (h-Numb) (Protein S171)	EBI-11150908	0.35
Q9ULH0	Kinase D-interacting substrate of 220 kDa (Ankyrin repeat-rich membrane-spanning protein)	EBI-11150908	0.35
P42566	Epidermal growth factor receptor substrate 15 (Protein Eps15) (Protein AF-1p)	EBI-11150908	0.35
Q13813	Spectrin alpha chain, non-erythrocytic 1 (Alpha-II spectrin) (Fodrin alpha chain) (Spectrin, non-erythroid alpha subunit)	EBI-11150908	0.35
Q96PK6	RNA-binding protein 14 (Paraspeckle protein 2) (PSP2) (RNA-binding motif protein 14) (RRM-containing coactivator activator/modulator) (Synaptotagmin-interacting protein) (SYT-interacting protein)	EBI-11150908	0.35
E9PK67	Poly [ADP-ribose] polymerase (PARP) (EC 2.4.2.-)	EBI-11150908	0.35
Q96FJ0	AMSH-like protease (AMSH-LP) (EC 3.4.19.-) (STAM-binding protein-like 1)	EBI-11150908	0.53
Q8IVT2	Mitotic interactor and substrate of PLK1 (Mitotic spindle positioning protein)	EBI-11150908	0.35
P53675	Clathrin heavy chain 2 (Clathrin heavy chain on chromosome 22) (CLH-22)	EBI-11150908	0.35
P09496	Clathrin light chain A (Lca)	EBI-11150908	0.35
P08047	Transcription factor Sp1	EBI-11150908	0.35
Q9H0K6	Pseudouridylate synthase PUS7L (EC 5.4.99.-) (Pseudouridylate synthase 7 homolog-like protein)	EBI-11150908	0.35
Q8WXE9	Stonin-2 (Stoned B)	EBI-11150908	0.35
P14384	Carboxypeptidase M (CPM) (EC 3.4.17.12)	EBI-11150908	0.35
P28066	Proteasome subunit alpha type-5 (Macropain zeta chain) (Multicatalytic endopeptidase complex zeta chain) (Proteasome zeta chain)	EBI-11150908	0.35
Q3B7T1	Erythroid differentiation-related factor 1	EBI-11150908	0.35
Q6ZRV2	Protein FAM83H	EBI-11150908	0.35
Q9UBH6	Xenotropic and polytropic retrovirus receptor 1 (Protein SYG1 homolog) (Xenotropic and polytropic murine leukemia virus receptor X3) (X-receptor)	EBI-11150908	0.35
O00159	Unconventional myosin-Ic (Myosin I beta) (MMI-beta) (MMIb)	EBI-11150908	0.35
Q16643	Drebrin (Developmentally-regulated brain protein)	EBI-11150908	0.35
Q5T0W9	Protein FAM83B	EBI-11150908	0.35
O00443	Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3K-C2-alpha) (PtdIns-3-kinase C2 subunit alpha) (EC 2.7.1.137) (EC 2.7.1.153) (EC 2.7.1.154) (Phosphoinositide 3-kinase-C2-alpha)	EBI-11150908	0.35
H0YEF7	Phosphatidylinositol-binding clathrin assembly protein	EBI-11150908	0.35
Q9NSY1	BMP-2-inducible protein kinase (BIKe) (EC 2.7.11.1)	EBI-11150908	0.35
F5GWT4	Non-specific serine/threonine protein kinase (EC 2.7.11.1)	EBI-11150908	0.35
Q8N3V7	Synaptopodin	EBI-11150908	0.35
P62158	Calmodulin-1	EBI-11150908	0.35
Q9UK73	Protein fem-1 homolog B (FEM1b) (FEM1-beta) (Fem-1-like death receptor-binding protein alpha) (Fem-1-like in apoptotic pathway protein alpha) (F1A-alpha)	EBI-11150908	0.35
P63010	AP-2 complex subunit beta (AP105B) (Adaptor protein complex AP-2 subunit beta) (Adaptor-related protein complex 2 subunit beta) (Beta-2-adaptin) (Beta-adaptin) (Clathrin assembly protein complex 2 beta large chain) (Plasma membrane adaptor HA2/AP2 adaptin beta subunit)	EBI-11150908	0.35
O94973	AP-2 complex subunit alpha-2 (100 kDa coated vesicle protein C) (Adaptor protein complex AP-2 subunit alpha-2) (Adaptor-related protein complex 2 subunit alpha-2) (Alpha-adaptin C) (Alpha2-adaptin) (Clathrin assembly protein complex 2 alpha-C large chain) (Huntingtin yeast partner J) (Huntingtin-interacting protein 9) (HIP-9) (Huntingtin-interacting protein J) (Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit)	EBI-11150908	0.35
Q13470	Non-receptor tyrosine-protein kinase TNK1 (EC 2.7.10.2) (CD38 negative kinase 1)	EBI-11150908	0.35
Q8TDG2	Actin-related protein T1 (ARP-T1)	EBI-11150908	0.35
O00291	Huntingtin-interacting protein 1 (HIP-1) (Huntingtin-interacting protein I) (HIP-I)	EBI-11150908	0.35
O95782	AP-2 complex subunit alpha-1 (100 kDa coated vesicle protein A) (Adaptor protein complex AP-2 subunit alpha-1) (Adaptor-related protein complex 2 subunit alpha-1) (Alpha-adaptin A) (Alpha1-adaptin) (Clathrin assembly protein complex 2 alpha-A large chain) (Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit)	EBI-11150908	0.35
Q9BY43	Charged multivesicular body protein 4a (Chromatin-modifying protein 4a) (CHMP4a) (SNF7 homolog associated with Alix-2) (SNF7-1) (hSnf-1) (Vacuolar protein sorting-associated protein 32-1) (Vps32-1) (hVps32-1)	EBI-11150908	0.35
P98082	Disabled homolog 2 (Adaptor molecule disabled-2) (Differentially expressed in ovarian carcinoma 2) (DOC-2) (Differentially-expressed protein 2)	EBI-11150908	0.35
P29703	Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha (PFTase/PGGTase I alpha) (EC 2.5.1.58) (EC 2.5.1.59) (CAAX farnesyltransferase subunit alpha) (FTase-alpha) (Ras proteins prenyltransferase subunit alpha) (Type I protein geranyl-geranyltransferase subunit alpha) (GGTase-I-alpha)	EBI-11525188	0.56
P56945	Breast cancer anti-estrogen resistance protein 1 (CRK-associated substrate) (Cas scaffolding protein family member 1) (p130cas)	EBI-15099463	0.35
Q8IWL3	Iron-sulfur cluster co-chaperone protein HscB (DnaJ homolog subfamily C member 20) [Cleaved into: Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic (C-HSC20); Iron-sulfur cluster co-chaperone protein HscB, mitochondrial]	EBI-13943458	0.35
Q6IQ23	Pleckstrin homology domain-containing family A member 7 (PH domain-containing family A member 7)	EBI-16398399	0.35
Q8TBP5	Membrane protein FAM174A (Hepatitis C virus NS5A-transactivated protein 6) (HCV NS5A-transactivated protein 6) (Transmembrane protein 157)	EBI-21502646	0.35
Q9P296	C5a anaphylatoxin chemotactic receptor 2 (Complement component 5a receptor 2) (G-protein coupled receptor 77)	EBI-21519943	0.35
Q8N3R9	Protein PALS1 (MAGUK p55 subfamily member 5) (Membrane protein, palmitoylated 5) (Protein associated with Lin-7 1)	EBI-21627185	0.35
Q9UJ41	Rab5 GDP/GTP exchange factor (RAP1) (Rabaptin-5-associated exchange factor for Rab5) (Rabex-5)	EBI-21644136	0.35
O95630	STAM-binding protein (EC 3.4.19.-) (Associated molecule with the SH3 domain of STAM) (Endosome-associated ubiquitin isopeptidase)	EBI-21667922	0.35
Q9NZL4	Hsp70-binding protein 1 (HspBP1) (Heat shock protein-binding protein 1) (Hsp70-binding protein 2) (HspBP2) (Hsp70-interacting protein 1) (Hsp70-interacting protein 2)	EBI-21711136	0.35
Q09019	Dystrophia myotonica WD repeat-containing protein (Dystrophia myotonica-containing WD repeat motif protein) (Protein 59) (Protein DMR-N9)	EBI-21714883	0.35
Q9ULV8	E3 ubiquitin-protein ligase CBL-C (EC 2.3.2.27) (RING finger protein 57) (RING-type E3 ubiquitin transferase CBL-C) (SH3-binding protein CBL-3) (SH3-binding protein CBL-C) (Signal transduction protein CBL-C)	EBI-21715242	0.35
Q8N2M8	CLK4-associating serine/arginine rich protein (Splicing factor, arginine/serine-rich 16) (Suppressor of white-apricot homolog 2)	EBI-21714908	0.35
Q96SL4	Glutathione peroxidase 7 (GPx-7) (GSHPx-7) (EC 1.11.1.9) (CL683)	EBI-21714987	0.35
Q9NSE4	Isoleucine--tRNA ligase, mitochondrial (EC 6.1.1.5) (Isoleucyl-tRNA synthetase) (IleRS)	EBI-21715157	0.35
Q01968	Inositol polyphosphate 5-phosphatase OCRL (EC 3.1.3.36) (EC 3.1.3.56) (Inositol polyphosphate 5-phosphatase OCRL-1) (OCRL-1) (Lowe oculocerebrorenal syndrome protein) (Phosphatidylinositol 3,4,5-triphosphate 5-phosphatase) (EC 3.1.3.86)	EBI-16412116	0.35
Q9Y6W8	Inducible T-cell costimulator (Activation-inducible lymphocyte immunomediatory molecule) (CD antigen CD278)	EBI-16721689	0.35
O43493	Trans-Golgi network integral membrane protein 2 (Trans-Golgi network glycoprotein 46) (TGN38 homolog) (hTGN46) (Trans-Golgi network glycoprotein 48) (hTGN48) (Trans-Golgi network glycoprotein 51) (hTGN51) (Trans-Golgi network protein 2)	EBI-16800265	0.27
Q9Y4C4	Malignant fibrous histiocytoma-amplified sequence 1 (Malignant fibrous histiocytoma-amplified sequence with leucine-rich tandem repeats 1)	EBI-20590176	0.44
Q9Y586	Protein mab-21-like 2	EBI-21261050	0.35
P49023	Paxillin	EBI-25376663	0.35
Q9C0B5	Palmitoyltransferase ZDHHC5 (EC 2.3.1.225) (Zinc finger DHHC domain-containing protein 5) (DHHC-5) (Zinc finger protein 375)	EBI-25637382	0.35
P51617	Interleukin-1 receptor-associated kinase 1 (IRAK-1) (EC 2.7.11.1)	EBI-28935882	0.35
Q9BYP7	Serine/threonine-protein kinase WNK3 (EC 2.7.11.1) (Protein kinase lysine-deficient 3) (Protein kinase with no lysine 3)	EBI-28946054	0.35
P31947	14-3-3 protein sigma (Epithelial cell marker protein 1) (Stratifin)	EBI-30814567	0.44
P04637	Cellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53)	EBI-34581131	0.35

Cross-References

Database	Links
UNIPROT	O14976 Q5U4P5 Q9BVY6
PDB	4C57 4C58 4C59 4O38 4Y8D 5Y7Z 5Y80
Pfam	PF00069 PF10409
PROSITE	PS51182 PS50076 PS51181 PS50011 PS00108
OMIM	602052
DisGeNET	2580

Cyclin-G-associated kinase (Homo sapiens)

General Information

Protein Information

Structure Viewer (PDB: 4C59)

Subcellular Location

Description Show

Position in the Nuclear Envelope

Membrane Topology

Assigned Ontology terms

Biology & Function

Description Hide

Assigned Ontology terms

Protein-Protein Interactions

Interactions with Nuclear Envelope proteins (9 interactors)

Interactions with other proteins (96 interactors)

Cross-References

Database

Links

Description

Description