Nucleoporin NSP1 - Saccharomyces cerevisiae S288C

Nucleoporin NSP1 (Saccharomyces cerevisiae S288C)

FASTA TEXT XML

Subcellular Location Biology & Function Interactions Cross-References

General Information

Protein Information
Protein Name	Nucleoporin NSP1
Accession Code	P14907
Gene	NSP1
Organism	Saccharomyces cerevisiae S288C (Taxonomy: 559292)
Part of Reference Proteome?	Yes
Sequence (Length: 823)
MNFNTPQQNKTPFSFGTANNNSNTTNQNSSTGAGAFGTGQSTFGFNNSAPNNTNNANSSITPAFGSNNTGNTAFGNSNPT SNVFGSNNSTTNTFGSNSAGTSLFGSSSAQQTKSNGTAGGNTFGSSSLFNNSTNSNTTKPAFGGLNFGGGNNTTPSSTGN ANTSNNLFGATANANKPAFSFGATTNDDKKTEPDKPAFSFNSSVGNKTDAQAPTTGFSFGSQLGGNKTVNEAAKPSLSFG SGSAGANPAGASQPEPTTNEPAKPALSFGTATSDNKTTNTTPSFSFGAKSDENKAGATSKPAFSFGAKPEEKKDDNSSKP AFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKPDENKASATSKPAFSF GAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKS DEKKDSDSSKPAFSFGTKSNEKKDSGSSKPAFSFGAKPDEKKNDEVSKPAFSFGAKANEKKESDESKSAFSFGSKPTGKE EGDGAKAAISFGAKPEEQKSSDTSKPAFTFGAQKDNEKKTEESSTGKSTADVKSSDSLKLNSKPVELKPVSLDNKTLDDL VTKWTNQLTESASHFEQYTKKINSWDQVLVKGGEQISQLYSDAVMAEHSQNKIDQSLQYIERQQDELENFLDNFETKTEA LLSDVVSTSSGAAANNNDQKRQQAYKTAQTLDENLNSLSSNLSSLIVEINNVSNTFNKTTNIDINNEDENIQLIKILNSH FDALRSLDDNSTSLEKQINSIKK

Structure Viewer (PDB: 7N9F)

Subcellular Location

Description
Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note=Symmetric distribution.
Position in the Nuclear Envelope
Location	Location ID	Description
Nuclear Envelope	SL-0178	The nuclear envelope is a membrane system which surrounds the nucleoplasm of eukaryotic cells. It is composed of the nuclear lamina, nuclear pore complexes and two nuclear membranes. The space between the two membranes is called the nuclear intermembrane space.
Nuclear Membrane	SL-0182	The membrane surrounding the nucleus. This term is used when it is not known if the protein is found in or associated with the inner or outer nuclear membrane.
Nuclear Pore Complex	SL-0185	The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active bidirectional transport of macromolecules such as proteins, RNAs etc across the double-membrane nuclear envelope.The NPC is composed of at least 30 distinct subunits known as Nucleoporins (NUPs).
Membrane Topology
Topology	Source	Annotation Type
Peripheral	UniProt	Sequence Analysis
Assigned Ontology terms

Cellular Component	Nuclear Envelope (GO:0005635) Nuclear Membrane (GO:0031965) Nuclear Periphery (GO:0034399) Nuclear Pore (GO:0005643) Nuclear Pore Central Transport Channel (GO:0044613) Nuclear Pore Nuclear Basket (GO:0044615)

Biology & Function

Description
Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NSP1 plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, pre-ribosome, signal recognition particle (SRP), and protein transport. {Experimental EvidencePubMed:10889207, Experimental EvidencePubMed:10952996, Experimental EvidencePubMed:11352936, Experimental EvidencePubMed:11387327, Experimental EvidencePubMed:11689687, Experimental EvidencePubMed:11739405, Experimental EvidencePubMed:12604785, Experimental EvidencePubMed:15039779, Experimental EvidencePubMed:9017593, Experimental EvidencePubMed:9461539, Experimental EvidencePubMed:9774653, Experimental EvidencePubMed:9843582, Experimental EvidencePubMed:9891088}.
Assigned Ontology terms

Biological Process	Cell Cycle (GO:0007049) NcRNA Export From Nucleus (GO:0097064) NLS-Bearing Protein Import Into Nucleus (GO:0006607) Nucleocytoplasmic Transport (GO:0006913) Poly(A)+ MRNA Export From Nucleus (GO:0016973) Protein Import Into Nucleus (GO:0006606) Ribosomal Large Subunit Export From Nucleus (GO:0000055) Ribosomal Small Subunit Export From Nucleus (GO:0000056) RNA Export From Nucleus (GO:0006405) TRNA Export From Nucleus (GO:0006409)
Molecular Function	Molecular Adaptor Activity (GO:0060090) Phospholipid Binding (GO:0005543) Structural Constituent Of Nuclear Pore (GO:0017056)

Protein-Protein Interactions

Interactions with Nuclear Envelope proteins (15 interactors)

Partner (NucEnvDB)		IntAct	Confidence score
P48837	Nucleoporin NUP57	EBI-390867	0.81
P40368	Nucleoporin NUP82	EBI-790236	0.87
Q02630	Nucleoporin NUP116/NSP116	EBI-795280	0.35
Q02199	Nucleoporin NUP49/NSP49	EBI-801342	0.68
P40477	Nucleoporin NUP159	EBI-805203	0.59
P36161	Nucleoporin NUP133	EBI-7438937	0.44
Q04175	Importin beta SMX1	EBI-1173446	0.40
P32499	Nucleoporin NUP2	EBI-6322490	0.00
Q05166	Nucleoporin ASM4	EBI-11888294	0.37
P61970	Nuclear transport factor 2	EBI-15747989	0.44
Q12315	mRNA export factor GLE1	EBI-849734	0.32
P40066	mRNA export factor GLE2	EBI-797307	0.44
Q14974	Importin subunit beta-1	EBI-1034546	0.62
Q06142	Importin subunit beta-1	EBI-1173482	0.72
P34077	Nucleoporin NIC96	EBI-789044	0.35
Interactions with other proteins (34 interactors)

Partner (UniProt)		IntAct	Confidence score
P02829	ATP-dependent molecular chaperone HSP82 (82 kDa heat shock protein) (Heat shock protein Hsp90 heat-inducible isoform)	EBI-763009	0.37
P38305	Altered inheritance of mitochondria protein 4 (Synthetic with old yellow enzyme protein 1)	EBI-761900	0.40
P51998	54S ribosomal protein YmL6, mitochondrial (Mitochondrial large ribosomal subunit protein uL4m)	EBI-811019	0.35
P10592	Heat shock protein SSA2	EBI-811019	0.53
P22353	54S ribosomal protein L8, mitochondrial (Mitochondrial large ribosomal subunit protein bL17m) (YmL8)	EBI-811019	0.35
Q06678	54S ribosomal protein L35, mitochondrial (Mitochondrial large ribosomal subunit protein mL38) (YmL35)	EBI-811019	0.35
P36516	54S ribosomal protein L3, mitochondrial (Mitochondrial large ribosomal subunit protein mL44) (YmL3)	EBI-811019	0.35
P36525	54S ribosomal protein L24, mitochondrial (Mitochondrial large ribosomal subunit protein bL28m) (YmL14/YmL24)	EBI-811019	0.35
P36523	54S ribosomal protein L15, mitochondrial (Mitochondrial large ribosomal subunit protein mL57) (YmL15)	EBI-811019	0.35
Q04599	54S ribosomal protein L1, mitochondrial (Mitochondrial large ribosomal subunit protein uL1m)	EBI-811019	0.35
P40005	Prefoldin subunit 2 (Genes involved in microtubule biogenesis protein 4) (Gim complex subunit 4) (GimC subunit 4)	EBI-8464369	0.56
P48363	Prefoldin subunit 3 (Genes involved in microtubule biogenesis protein 2) (Gim complex subunit 2) (GimC subunit 2)	EBI-8464653	0.56
P29295	Casein kinase I homolog HRR25 (EC 2.7.11.1)	EBI-2612057	0.35
P23291	Casein kinase I homolog 1 (EC 2.7.11.1)	EBI-2614250	0.35
P24869	G2/mitotic-specific cyclin-2	EBI-2617071	0.35
P26448	Mitotic check point protein BUB2 (Cell cycle arrest protein BUB2)	EBI-2882390	0.00
P22696	Peptidyl-prolyl cis-trans isomerase ESS1 (PPIase ESS1) (EC 5.2.1.8) (Parvulin ESS1) (Processing/termination factor 1)	EBI-2883245	0.00
Q02796	Transcriptional regulatory protein LGE1 (Large cells protein 1)	EBI-2885546	0.00
P39723	Spindle pole component SPC72	EBI-2887969	0.00
Q04477	Kinetochore protein SPC24	EBI-2887856	0.00
P48353	Protein HLJ1	EBI-3658592	0.35
P10591	Heat shock protein SSA1 (Heat shock protein YG100)	EBI-3673674	0.35
P11484	Ribosome-associated molecular chaperone SSB1 (EC 3.6.4.10) (Cold-inducible protein YG101) (Heat shock protein SSB1) (Hsp70 chaperone Ssb)	EBI-3691838	0.35
P52553	Prefoldin subunit 6 (Genes involved in microtubule biogenesis protein 1) (Gim complex subunit 1) (GimC subunit 1)	EBI-3739443	0.35
P53900	Prefoldin subunit 4 (Genes involved in microtubule biogenesis protein 3) (Gim complex subunit 3) (GimC subunit 3)	EBI-3739619	0.35
P33416	Heat shock protein 78, mitochondrial	EBI-3745755	0.35
P15992	Heat shock protein 26 (26 kDa heat shock protein)	EBI-3750055	0.35
P38915	Transcription factor SPT8	EBI-4375569	0.35
P50875	Transcription factor SPT20	EBI-4376349	0.35
Q03330	Histone acetyltransferase GCN5 (EC 2.3.1.48) (Histone crotonyltransferase GCN5) (EC 2.3.1.-)	EBI-4377624	0.35
Q02336	Transcriptional adapter 2	EBI-4378994	0.35
P50102	Ubiquitin carboxyl-terminal hydrolase 8 (EC 3.4.19.12) (Deubiquitinating enzyme 8) (Ubiquitin thioesterase 8) (Ubiquitin-specific-processing protease 8)	EBI-4379349	0.35
P36160	Ribosome biogenesis protein RPF2	EBI-7433031	0.35
Q8IYF3	Testis-expressed protein 11 (Protein ZIP4 homolog) (ZIP4H)	EBI-11523349	0.56

Cross-References

Database	Links
UNIPROT	P14907 D6VWE3
PDB	1O6O 7N85 7N9F 7WOO 7WOT
Pfam	PF05064

Nucleoporin NSP1 (Saccharomyces cerevisiae S288C)

General Information

Protein Information

Structure Viewer (PDB: 7N9F)

Subcellular Location

Description Show

Position in the Nuclear Envelope

Membrane Topology

Assigned Ontology terms

Biology & Function

Description Hide

Assigned Ontology terms

Protein-Protein Interactions

Interactions with Nuclear Envelope proteins (15 interactors)

Interactions with other proteins (34 interactors)

Cross-References

Database

Links

Description

Description