Nuclear mRNA export protein SAC3 - Saccharomyces cerevisiae S288C

Nuclear mRNA export protein SAC3 (Saccharomyces cerevisiae S288C)

FASTA TEXT XML

Subcellular Location Biology & Function Interactions Cross-References

General Information

Protein Information
Protein Name	Nuclear mRNA export protein SAC3
Accession Code	P46674
Gene	SAC3
Organism	Saccharomyces cerevisiae S288C (Taxonomy: 559292)
Part of Reference Proteome?	Yes
Sequence (Length: 1301)
MNTSFGSVVPSTNFNFFKGHGNNDNTSANSTVNNSNFFLNSNETKPSKNVFMVHSTSQKKSQQPLQNLSHSPSYTENKPD KKKKYMINDAKTIQLVGPLISSPDNLGFQKRSHKARELPRFLINQEPQLEKRAFVQDPWDKANQEKMISLEESIDDLNEL YETLKKMRNTERSIMEEKGLVDKADSAKDLYDAIVFQGTCLDMCPTFERSRRNVEYTVYSYEKNQPNDKKASRTKALKVF ARPAAAAAPPLPSDVRPPHILVKTLDYIVDNLLTTLPESEGFLWDRMRSIRQDFTYQNYSGPEAVDCNERIVRIHLLILH IMVKSNVEFSLQQELEQLHKSLITLSEIYDDVRSSGGTCPNEAEFRAYALLSKIRDPQYDENIQRLPKHIFQDKLVQMAL CFRRVISNSAYTERGFVKTENCLNFYARFFQLMQSPSLPLLMGFFLQMHLTDIRFYALRALSHTLNKKHKPIPFIYLENM LLFNNRQEIIEFCNYYSIEIINGDAADLKTLQHYSHKLSETQPLKKTYLTCLERRLQKTTYKGLINGGEDNLASSVYVKD PKKDRIPSIADQSFLMENFQNNYNEKLNQNSSVKPQINTSPKRVATRPNHFPFSQESKQLPQISQSHTLSTNPLLTPQVH GDLSEQKQQQIKTVTDGGSPFVFDQSAQNSTVEASKAHMISTTSNGAYDEKLSSEQEEMRKKEEQRIEEEKTQLKKKQEN ADKQVITEQIANDLVKEVVNSSVISIVKREFSEANYRKDFIDTMTRELYDAFLHERLYLIYMDSRAELKRNSTLKKKFFE KWQASYSQAKKNRILEEKKREEIKLVSHQLGVPGFKKSTCLFRTPYKGNVNSSFMLSSSDKNLIFSPVNDEFNKFATHLT KISKLWRPLEMQSIYYDNLTKKFPSNSLTPANLFIYAKDWTSLSNRWILSKFNLQTAQDSKKFSNNIISSRIICIDDEYE PSDFSDLQLLIFNTGVTNPDIFDLEMKLKDDGEELIKLITGISLNTNICFSLLIIYWESAENTLSESTIKHLLKLNRISK NYSSVIERIDLMNLTEESPHKCLEDKLSEISHSYVYKLTERGKYDKTLRQKRSLAGIHSRSTQLQTTKDIDQKMKKMLEK EKNKYQQQIGERNTYAHLESHIDASPRSKKRKLPILLSTSHSSQFKTPLASRLNTSGSSTSPPLPSHLAMKFRKNSRVTS LHTVLPVSTPSHSNNIPAASFSGNNTTDIQSQQLIENQKSTSVYLNNVSERILGNQEICQTPINPVTPVLDGADQGKEDI PDSILELKILIDSVKKKVNND

Structure Viewer (PDB: 3FWB)

Subcellular Location

Description
Nucleus envelope {Experimental EvidencePubMed:12411502, Experimental EvidencePubMed:14562095}. Note=Localizes to the nuclear pores.
Position in the Nuclear Envelope
Location	Location ID	Description
Nuclear Envelope	SL-0178	The nuclear envelope is a membrane system which surrounds the nucleoplasm of eukaryotic cells. It is composed of the nuclear lamina, nuclear pore complexes and two nuclear membranes. The space between the two membranes is called the nuclear intermembrane space.
Membrane Topology
Topology	Source	Annotation Type
Unknown	UniProt	Sequence Analysis
Assigned Ontology terms

Cellular Component	Cytoplasm (GO:0005737) Nuclear Pore Cytoplasmic Filaments (GO:0044614) Nucleus (GO:0005634) Transcription Export Complex 2 (GO:0070390)

Biology & Function

Description
Component of the SAC3-THP1 complex, which functions in transcription-coupled mRNA export from the nucleus to the cytoplasm. SAC3-THP1 functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), by association with components of the nuclear mRNA export machinery (MEX67-MTR2 and SUB2) in the nucleoplasm and the nucleoporin NUP1 at the nuclear basket. {Experimental EvidencePubMed:12411502, Experimental EvidencePubMed:12702719}.
Assigned Ontology terms

Biological Process	Actin Filament-Based Process (GO:0030029) Mitotic Cell Cycle (GO:0000278) MRNA 3'-End Processing (GO:0031124) MRNA Export From Nucleus (GO:0006406) Nuclear MRNA Surveillance (GO:0071028) Positive Regulation Of Transcription By RNA Polymerase II (GO:0045944) Post-Transcriptional Tethering Of RNA Polymerase II Gene DNA At Nuclear Periphery (GO:0000973) Protein Export From Nucleus (GO:0006611) Ribosomal Small Subunit Biogenesis (GO:0042274) Transcription-Coupled Nucleotide-Excision Repair (GO:0006283)
Molecular Function	Molecular Adaptor Activity (GO:0060090)

Protein-Protein Interactions

Interactions with Nuclear Envelope proteins (4 interactors)

Partner (NucEnvDB)		IntAct	Confidence score
Q08231	Nuclear mRNA export protein THP1	EBI-797734	0.88
P06704	Cell division control protein 31	EBI-7945717	0.77
P25491	Mitochondrial protein import protein MAS5	EBI-3766328	0.35
P20676	Nucleoporin NUP1	EBI-2124372	0.40
Interactions with other proteins (23 interactors)

Partner (UniProt)		IntAct	Confidence score
P40073	High osmolarity signaling protein SHO1 (Osmosensor SHO1) (Suppressor of SUA8-1 mutation) (Synthetic high osmolarity-sensitive protein 1)	EBI-601163	0.37
P50101	Ubiquitin carboxyl-terminal hydrolase 15 (EC 3.4.19.12) (Deubiquitinating enzyme 15) (Ubiquitin thioesterase 15) (Ubiquitin-specific-processing protease 15)	EBI-7110957	0.40
P13517	F-actin-capping protein subunit beta	EBI-7228158	0.40
Q04062	26S proteasome regulatory subunit RPN9 (Proteasome non-ATPase subunit 7)	EBI-7650434	0.40
P39010	Palmitoyltransferase AKR1 (EC 2.3.1.225) (Ankyrin repeat-containing protein AKR1)	EBI-7897739	0.44
Q6WNK7	Transcription and mRNA export factor SUS1	EBI-1370393	0.82
Q99257	mRNA export factor MEX67	EBI-2124319	0.50
P34232	mRNA transport regulator MTR2	EBI-2124319	0.40
Q07478	ATP-dependent RNA helicase SUB2 (EC 3.6.4.13) (Suppressor of BRR1 protein 2)	EBI-2124381	0.40
P38811	Transcription-associated protein 1 (p400 kDa component of SAGA)	EBI-2213151	0.40
P35177	Transcriptional activator SPT7	EBI-2213151	0.40
Q06410	Autophagy-related protein 17	EBI-2345986	0.37
Q06677	Auxilin-like clathrin uncoating factor SWA2 (Bud site selection protein 24) (DnaJ-related protein SWA2) (J protein SWA2) (Synthetic lethal with ARF1 protein 2)	EBI-3758909	0.35
P10591	Heat shock protein SSA1 (Heat shock protein YG100)	EBI-3773334	0.35
P11484	Ribosome-associated molecular chaperone SSB1 (EC 3.6.4.10) (Cold-inducible protein YG101) (Heat shock protein SSB1) (Hsp70 chaperone Ssb)	EBI-3783327	0.35
P39987	Heat shock protein SSC3, mitochondrial (Extracellular mutant protein 10)	EBI-3788346	0.35
P38788	Ribosome-associated complex subunit SSZ1 (DnaK-related protein SSZ1) (Heat shock protein 70 homolog SSZ1) (Pleiotropic drug resistance protein 13)	EBI-3791492	0.35
P40150	Ribosome-associated molecular chaperone SSB2 (EC 3.6.4.10) (Heat shock protein SSB2) (Hsp70 chaperone Ssb)	EBI-3801253	0.35
P32589	Heat shock protein homolog SSE1 (Chaperone protein MSI3)	EBI-3807444	0.35
P47079	T-complex protein 1 subunit theta (TCP-1-theta) (CCT-theta)	EBI-3824057	0.35
P38129	Transcription initiation factor TFIID subunit 5 (TAFII-90) (TBP-associated factor 5) (TBP-associated factor 90 kDa)	EBI-4389629	0.35
Q05027	Transcription initiation factor TFIID subunit 9 (TAFII-17) (TAFII20) (TBP-associated factor 17 kDa) (TBP-associated factor 9)	EBI-4391019	0.35
O94742	26S proteasome complex subunit SEM1	EBI-15970376	0.61

Cross-References

Database	Links
UNIPROT	P46674 D6VSD9
PDB	3FWB 3FWC 3T5V 4C31 4MBE 4TRQ 5G5P 5L3T
Pfam	PF12209 PF03399
PROSITE	PS50250

Nuclear mRNA export protein SAC3 (Saccharomyces cerevisiae S288C)

General Information

Protein Information

Structure Viewer (PDB: 3FWB)

Subcellular Location

Description Show

Position in the Nuclear Envelope

Membrane Topology

Assigned Ontology terms

Biology & Function

Description Hide

Assigned Ontology terms

Protein-Protein Interactions

Interactions with Nuclear Envelope proteins (4 interactors)

Interactions with other proteins (23 interactors)

Cross-References

Database

Links

Description

Description